CÜNEYT SUZER, Deniz ÇOBAN, ŞÜKRÜ YILDIRIM, MÜGE ALİYE HEKİMOĞLU, H. Okan KAMACI, Kürşat FIRAT, ŞAHİN SAKA

Stage-Specific Ontogeny of Digestive Enzymes in the Cultured Common Dentex (Dentex dentex) Larvae

Bu çalışmada, sinarit (Dentex dentex) larvalarında açılımdan 45. güne kadar geçen erken dönem boyunca önemli sindirim enzimlerinin dönemsel ontogenileri notokorda bükülmesine göre incelenmiştir. Besin keseli sinarit larvalarında notokorda düz yapıda gözlenmiş ve bükülme öncesi dönem 0 ile 18. günler arasında 2,75 ile 6,27 mm total boy uzunlukları arasında izlenmiştir. Ardından, notokordanın bükülmesi ise 19 ile 30. günler arasında 6,27 ile 10,9 mm total boy uzunlukları arasında tespit edilmiştir. Çalışma boyunca izlenen sindirim enzimleri aktivitelerindeki önemli değişimlerin çoğunlukla metamorfoz ve toz yeme geçiş süreci ile ilişkili olduğu bulunmuştur. Tripsin ve kimotripsin speisifk aktiviteleri larvalarda ağız açılımı ile birlikte ilk olarak 3. günde tespit edilmiş ve 25. güne kadar yavaş bir artış göstermiştir. Pepsin aktivitesi ise mide oluşumu ile eş zamanlı olarak ilk kez 28. günde gözlenmiş ve 30. güne kadar ani bir artış sergilemiştir. Amilaz ve lipaz aktivitesi ise sırasıyla 2. ve 4. günlerde izlenmiş ve sonrasında larval dönemin ilk haftası boyunca hızlı şekilde yükselmiştir. Ayrıca, pepsin dışındaki tüm sindirim enzimleri aktivitesi ilk olarak bükülme öncesi dönemde izlenmiş ve gastrik salgının başlangıcı ve fonksiyonel sindirim sisteminin oluşması sırasıyla bükülme ve bükülme sonrası dönemde tespit edilmiştir.

Kültürü Yapılan Sinarit (Dentex dentex) Larvalarında Sindirim Enzimlerinin Dönemsel Gelişimi

Stage-specific ontogeny of main digestive enzymes in common dentex, Dentex dentex, were assayed during early life development according to notochord flexion studied from hatching to 45 days after hatching (DAH). The notochord was straight in yolk-sac larvae of D. dentex and preflexion stage was observed between 0 and 18 DAH as 2.75 and 6.27 mm at total length (TL). Then, flexion of the notochord was estimated between 19 and 30 DAH as 6.27 and 10.9 mm at TL. Important alterations in specific activities of all digestive enzymes measured during period of this study were mostly related with metamorphosis and co-feeding. Trypsin and chymotrypsin specific activities were firstly detected on day 3 and were related with mouth opening, whereas they slightly increased until 25 DAH respectively. Pepsin was firstly detected on day 28 and related with stomach formation and a sharp increase was observed until 30 DAH. Both amylase and lipase were measured on day 2 and 4, respectively and they also sharply increased during the first week of life. Additionally, except pepsin, most of the digestive enzyme activities were firstly secreted in the preflexion stage and also gastric secretion started and functional development digestive system were occurred in in flexion and postflexion stages, respectively.

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