Suman Bhusan CHAKRABORTY, Tamás MOLNÁR, László ARDÓ, Galina JENEY, Csaba HANCZ

Oral Administration of Basella alba Leaf Methanol Extract and Genistein Enhances the Growth and Non-Specific Immune Responses of Oreochromis niloticus

The effects of the isoflavone genistein and methanol extract of Basella alba leaves were evaluated in Nile tilapia, Oreochromis niloticus on growth and immunostimulation. Adult tilapia (mean weight 39.55 g) was fed diets containing genistein (1 gm/kg) and methanol extract of B. alba (1 g/kg) for 35 days. Basella alba extract treated tilapia showed significantly higher (P<0.05) weight gain, respiratory burst, phagocytic activity, plasma protein content and plasma lysozyme activity compared to fish fed control diet. The B. alba extract treated fish showed the highest final individual mean body weight, final individual length, specific growth rate, hepatosomatic index and total immunoglobulin content. The phagocytic and respiratory burst activities of genistein treated fish were significantly higher (P<0.05) compared to the control group. The weight gain, lysozyme activity and total protein content of genistein treated fish did not differ significantly (P>0.05) from both control and B. alba treated fish. There was no significant difference (P>0.05) in the food conversion ratio as well among the different treatment groups. The study indicates that B. alba methanol extract might positively influence the growth and protect the health status of tilapia.

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Bamidele, O., Akinnuga, A.M., Olorunfemi, J.O., Odetola, O.A., Oparaji, C.K. and Ezeigbo, N. 2010. Effects of aqueous extract of Basella alba leaves on haematological and biochemical parameters in albino rats. African Journal of Biotechnology, 9(41): 6952- 6955. doi: 10.5897/AJB10.957.

Bennetau-Pelissero, C., Breton, B.B., Bennetau, B., Corraze, G., Le Menn, F., Davail-Cuisset, B., Helou, C. and Kaushik, S.J. 2001. Effect of genisteinenriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss. General and Comparative Endocrinology, 121(2): 173-187. doi:10.1006/gcen.2000.7585

Bricknell, I. and Dalmo, R.A. 2005. The use of immunostimulants in fish larval aquaculture. Fish and Shellfish Immunology, 19(5): 457-472. doi: 10.1016/j.fsi.2005.03.008

Chakraborty, S.B. and Hancz, C. 2011. Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in finfish culture. Reviews in Aquaculture, 3: 103-119. doi: 10.1111/j.1753-5131. 2011.01048.x

Chakraborty, S.B., Horn, P. and Hancz, C. 2014. Application of phytochemicals as growth-promoters and endocrine modulators in fish culture. Reviews in Aquaculture, 6: 1-19. doi: 10.1111/raq.12021

Citarasu, T. 2010. Herbal biomedicines: a new opportunity for aquaculture industry. Aquaculture International, 18: 403414. doi: 10.1007/s10499-009-9253-7 Food and Agriculture Organization of the United Nations (FAO). 2012. The State of World Fisheries and Aquaculture, Rome.

Green, C.C. and Kelly, A.M. 2009. Effects of the estrogen mimic genistein as a dietary component on sex differentiation and ethoxyresorufin-O-deethylase (EROD) activity in channel catfish (Ictalurus punctatus). Fish Physiology and Biochemistry, 35: 377384. doi: 10.1007/s10695-008-9260-z

Grubben, G.J.H. and Denton, O.A. 2004. Plant Resources of Tropical Africa 2. Vegetable. PROTA Foundation, Wageningen; Backhuys, Leiden; CTA, Wageningen, 4: 103-111.

Ingham, R.R., Gesualdi, D.A., Toth, C.R. and Clotfelter, E.D. 2004. Effects of genistein on growth and development of aquatic vertebrates. Bulletin of Environmental Contamination and Toxicology, 72: 625-631. doi: 10.1007/s00128-004-0289-0.

Jeney, G., Yin, G., Ardó, L. and Jeney, Z. 2009. The use of immunostimulating herbs in fish. An overview of research. Fish Physiology and Biochemistry, 35: 669 676. doi: 10.1007/s10695-009-9304-z Ji, S-C., Takaoka, O., Lee, S-W., Hwang, J-H., Kim, Y-S., Ishimaru, K., Seoka, M., Jeong, G-S. and Takii, K. 2009. Effect of dietary medicinal herbs on lipid metabolism and stress recovery in red sea bream Pagrus major. Fisheries Science, 75: 665672. doi: 10.1007/s12562-009-0058-4

Kim, D. and Austin, B. 2006. Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish and Shellfish Immunology, 21: 513524. doi: 10.1016/j.fsi.2006.02.007

Kumar, A. and Bhardwaj, A. 2012. Comparative, qualitative and quantitative chemotypic characterization among north Indian Tribulus terrestris. International Research Journal of Pharmacy, 3(6): 212-218.

Magnadóttir, B. 2006. Innate immunity of fish (overview). Fish and Shellfish Immunology, 20: 137151. doi: 10.1016/j.fsi.2004.09.006

Maisuthisakul, P., Pongsawatmanit, R. and Gordon, M.H. 2007. Assessment of phenolic content and free-radical scavenging capacity of some Thai indigenous plants. Food Chemistry, 100(4): 14091418. doi: 10.1016/j.foodchem.2005.11.032

Malpani, M.O., Rajput, P.R., Mane, V.D. and Dhabe, P.S. 2011. Phytochemical screening, anifungal activity and curative impact on Channa punctatus fish of Butea monosperma (Lam): flower, leaves and gum. Der Pharmacia Lettre, 3(5): 271-280.

Masuda, T., Yonemori, S., Oyama, Y., Takeda, Y., Tanaka, T. and Andoh, T. 1999. Evaluation of the antioxidant activity of environmental plants: activity of the leaf extracts from seashore plants. Journal of Agricultural and Food Chemistry, 47(4): 17491754. doi: 10.1021/jf980864s

Moundipa, P.F., Beboy, N.S.E., Zelefack, F., Ngoula, S., Tsamo, E., Schill, W-B. and Monsees, T.K. 2005. Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells. Asian Journal of Andrology, 7(4): 411-417. doi: 10.1111/j.1745-7262.2005.00056.x

Nantia, E.A., Travert, C., Manfo, F-P.T., Carreau, S., Monsees, T.K. and Moundipa, P.F. 2011. Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells. International Journal of Molecular Sciences, 12(1): 376-384. doi:10.3390/ijms12010376

Pechsiri, J. and Yakupitiyage, A. 2005. A comparative study of growth and feed utilization efficiency of sexreversed diploid and triploid Nile tilapia, Oreochromis niloticus L. Aquaculture Research, 36(1): 45-51. doi: 10.1111/j.1365-2109.2004.01182.x.

Ray, S., Chatterjee, S. and Chakrabarti, C.S. 2013. Antiprolifertive activity of allelochemicals present in aqueous extract of Synedrella nodiflora (L.) Gaertn. in apical meristems and Wistar rat bone marrow cells. IOSR Journal of Pharmacy, 3(2): 1-10. doi: 10.9790/3013-3220110

Sadekarpawar, S. and Parikh, P. 2013. Gonadosomatic and hepatosomatic indices of freshwater fish Oreochromis mossambicus in response to a plant nutrient. World Journal of Zoology, 8(1): 110-118. doi: 10.5829/idosi.wjz.2013.8.1.7268

Sankaran, K. and Gurnani, S. 1972. On the variation in the catalytic activity of lysozyme in fishes. Indian Journal of Biochemistry & Biophysics, 9(2): 62165.

Secombes, C.J. 1990. Isolation of salmonid macrophages and analysis of their killing activity. In: J.S. Stolen, D.P. Anderson, B.S. Robertson, W.B. van Miuswinkell (Eds.), Techniques in Fish Immunology, SOS Publications, Fair Haven, New Jersey, USA: 137154.

Seeley, K.R., Gillespie, P.D. and Weeks, B.A. 1990. A simple technique for the rapid spectrophotometric determination of phagocytosis by fish macrophages. Marine Environmental Research, 30(1): 37-41. doi: 10.1016/0141-1136(90)90009-D

Siriwatanametanon, N., Fiebich, B.L., Efferth, T., Prietoa, J.M. and Heinrich, M. 2010. Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities. Journal of Ethnopharmacology, 130(2): 196207. doi: 10.1016/j.jep.2010.04.036

Sivasankar, V., Moorthi, A., Sarathi Kannan, D. and Suganya devi, P. 2011. Anthocyanin, and its antioxidant properties in Basella alba. Journal of Pharmacy Research; 4(3): 800-806.

Turan, F. and Akyurt, I. 2005. Effects of androstenedione, a phytoandrogen, on growth and body composition in the African catfish Clarias gariepinus. The Israeli Journal of Aquaculture Bamidgeh, 57(1): 62-66.

Zhang, L., Khan, I.A., Willett, K.L. and Foran, C.M. 2003. In vivo effects of black cohosh and genistein on estrogenic activity and lipid peroxidation in Japanese Medaka (Oryzias latipes). Journal of Herbal Pharmacotherapy, 3(3): 33-50. doi: 10.1080/J157v03n03_04