Fulya BENZER, MEHTAP ÖZÇELİK, NURAN CIKCIKOĞLU YILDIRIM

The Effects of Dietary Antioxidants on the Arginase Activity and Nitric Oxide Level of Narrow-Clawed Turkish Crayfish (Astacus leptodactylus, Esch. 1823) in Moulting Period

Diyet antioksidanları (vitamin E, vitamin C, astaxantin, ? karoten)'nın tatlı su kereviti (Actacus leptodactylus) kas ve hepatopankreası arjinaz aktivitesi ile nitrik oksit seviyeleri üzerine etkileri kabuk değişimi döneminde araştırılmıştır. Deneysel diyetlere 150 mg kg-1 vitamin E, 200 mg kg-1 vitamin C, 200 mg kg-1 Astaxhantin and 200 mg kg-1 ? karoten eklendi. A. leptodactylus 75 gün boyunca bu diyetler ile toplam ağırlığının 2% oranında beslendi. Kontrol ile kıyaslandığında Arjinaz aktivitesi kerevit kasında VC, VE, astaxanthin ve ? karoten ile artmıştır ancak hepatopankreasda arjinaz aktivitesi ? karotene ile artmıştır. Kas NO seviyeleri kontrolle kıyaslandığında azalmıştır ama bu azalış istatistiksel olarak önemli değildir. VC ve astaxanthin ile beslenen kerevit hepatopankreasından NO düzeyleri kontrol gruplarına göre daha yüksek bulunmuştur. Sonuçlarımız diyetsel antioksidanların kerevitlerin kabuk değiştirme döneminde arjinaz aktiviteleri ve NO düzeylerini değiştirdiğini kanıtlamıştır.

The effects of dietary antioxidants (vitamine E, vitamine C, Astaxhantin, ? carotene) on arginase activity and nitric oxide levels of hepatopancreas and muscle of freshwater crayfish (Actacus leptodactylus) were investigated during moulting.150 mg kg-1 vitamine E (VE), 200 mg kg-1vitamine C (VC), 200 mg kg-1 Astaxhantin and 200 mg kg-1 ? carotene were added to experimental diets. A. leptodactylus with these diets were fed with 2% of their total weight daily, in a day during 75 days. Arginase activity was increased in muscle of crayfish fed the diets VC, VE, astaxanthin and ? karoten but in hepatopancreas arginase activity was increased with ? carotene when compared to control. NO levels in muscle were decreased when compared to control but this decrease was not statically significant. NO levels in hepatopancreas of crayfish fed the diets VC and astaxanthin were higher than control groups. Our results demonstrated that dietary antioxidants affect the arginase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus, Esch. 1823) during moulting.

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