Girish KUMAR, Swaraj Priyaranjan KUNAL, Maria Rosalia MENEZES

Low genetic variation suggest single stock of kawakawa Euthynnus affinis (Cantor, 1849) along the Indian coast

Kawakawa Euthynnus affinis is an epipelagic migratory tuna species, widely distributed in the tropical and subtropical waters of the Indo-Pacific region. It constitutes the largest tuna fishery in Indian waters. In present study, restriction fragment length polymorphism (RFLP) analysis of the mitochondrial D-loop region was employed to examine the levels of genetic diversity among kawakawa samples collected from eight main fishing zones (Veraval (VE), Ratnagiri (RA), Kochi (KO), Kavaratti (KA), Port-Blair (PB), Tuticorin (TU), Pondicherry (PO) and Vizag (VI)) along the Indian coast. A 500 bp segment of mitochondrial D-loop region was screened in 400 samples using six restriction enzymes (Rsa I, Alu I, Hinf I, Hha I, Msp I and Hae III), resulting in 13 composite morphs. Analysis of molecular variance (AMOVA) of mtDNA data revealed no significant genetic differentiation among sites (FST = -0.00446, P = 0.84946). Results of the genetic analyses of present study suggest the single stock of kawakawa along the Indian coast.

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