NÜKET SİVRİ, Cemal SANDALLI, OSMAN BİROL ÖZGÜMÜŞ, Feyza COLAKOGLU, Dilek DOĞAN

Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (Istanbul Turkey)

Bu çalışmanın amacı, Küçükçekmece Lagünündeki (İstanbul) fekal bakteri yoğunluğunu bulmak ve izole edilen Gram negatif basillerin antibiyotiklere direncini analiz etmektir. Örnekler, Haziran 2006'dan Haziran 2008 tarihleri arasında aylık olarak alınmış ve toplam 232 Gram negatif basil izole edilmiştir. Antimikrobial hassasiyet testleri için kloramfenikol, tetrasiklin, nalidiksik asit, ampisilin, imipenem, seftazidim, amikasin, streptomisin ve amoksisilin+klavulanik asit kullanılmıştır. Trimetoprim+sulfametoksazola yatkınlık da sadece integron taşıyan organizmalar için incelenmiştir. Antibiyotik direnç sonuçlarına göre bakterilerin en dirençli olduğu antibiyotik ampisilin (%76,29) ve en hassas olduğu antibiyotik ise amikasin (%6,44) olarak bulunmuştur. 232 izolatın 20 si (%8,6) sınıf 1 ve / veya sınıf 2 integron barındırmıştır. DNA sekanslama sonucunda, integronlara ait değişken bölgelerin barındırdığı gen kasetlerinin, DfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, blaOXA-30 ve sat2 olduğu tespit edilmiştir. Bakterilere ait bu integronlar 12 ve D3 nolu istasyonlar dışındaki tüm örnekleme bölgelerinde bulunmuştur. Bu çalışmada, Küçükçekmece Lagününde Gram negatif bakteri türlerinin identifikasyonu ve antibiyotik direnç profili ile ilgili çalışma ilk kez yapılmıştır. Muhtemelen yoğun antropojenik girdilerin sonucu olarak Lagünde ağır bir fekal kirlilik olduğu görülmüştür. Atık su arıtma tesisleri gibi etkili önlemler alınmazsa, insanların normal mikrobiotasında yer alan bakteri izolatlarına, antibiyotik direnç genlerinin aktarımı ile halk sağlığı riski oluşması olasıdır.

Küçükçekmece Lagünü nden (İstanbul- Türkiye) izole edilen enterik bakterilerin antibiyotik direnç profilleri

The aim of this study is to find out the density of the fecal bacteria and to analyze resistance to antimicrobials of Gram negative bacilli isolated from the Kucukcekmece Lagoon, Istanbul. Samples were taken monthly from June 2006 to June 2008 and a total of 232 Gram negative bacilli were isolated. Chloramphenicol, tetracycline, nalidixic acid, ampicillin, imipenem, ceftazidime, amikacin, streptomycin and amoxicillin + clavulanic acid were used in antimicrobial susceptibility tests. Susceptibility to trimethoprim + sulfamethoxazole was also examined in only integron-bearing organisms. The antibiotic resistance tests resulted in bacteria being the most resistant against ampicillin (76.29%) and the most sensitive against amikacin (93.56%). Of 232 isolates, 20 (8.6%) coliforms harbored class 1 and/or class 2 integrons. DNA sequencing showed that variable regions of the integrons harbored various gene cassettes; dfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, blaOXA-30 and sat2. Integrons were found in bacteria from all sampling areas except 12 and D3. In this study, the determination of bacterial identification of the species of Gram negative bacilli and their Antibiotic Resistance Profiles in the Kucukcekmece Lagoon for the first time was investigated. A finding indicates that there is a heavy fecal pollution in this lagoon environment, which might probably be resulted from the intensive anthropogenic facilities. The risk to public health could be the transfer of antibiotic resistance determinants from the bacterial isolates to normal microbiota bacteria of humans unless the effective precautions such as water treatment plants are taken.

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