Impact of Fed Containing Different Levels of Diets Supplementation Spirulina platensis on Growth, Haematological, Body Composition and Biochemical Parameters, of Gurami (Osphronemus gouramy)

Spirulina platensis (S. platensis) is a blue-green algae, shaped like a spiral, contains high nutritional value includingproteins, vitamins, minerals and essential amino acids. The objective of this study is to evaluate the effects of supplementationS. platensis in diets and to get the level of supplementation S. platensis in diets gurami fish, Osphronemus gouramy bestpromote growth, hematological, body composition and biochemical blood parameters. The fish are used to measure theaverage weight 2.52 ± 0.17 g. The treatments tested was 5 groups, replicated 3 times for each group. The dietary levels S.platensis of five treatment group were: C (control group), P1, P2, P3, P4, respectively supplementation S. platensis 0, 2, 3, 4,and 6 g/kg of diet, the trial period is 56 days.The results showed that the level difference S. platensis supplementation canincrease growth, hematological and biochemical blood parameters gurami. Best growth, body composition, hematological andbiochemical blood parameters of gurami is obtained at the level of supplementation S. platensis 6 g/kg of diet. Findings fromthis study indicate that supplementation S. platensis 6 g/kg of diet in aquaculture gurami increasing growth, bodycomposition and immunity body fish.

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Abd El Baky, H.H., El Baroty, G.S., & Ibrahem, E.A. (2015). Functional characters evaluation of biscuits sublimated with pure phycocyanin isolated from Spirulina and Spirulina biomass. Nutricion Hospitalaria, 32(1), 231-241. http://dx.doi.org/10.3305/nh.2015.32.1.8804

Abdelkhalek, N.K.M., Ghazy, E.W., & Abdel-Daim, M.M. (2015). Pharmacodynamic interaction of Spirulina platensis and deltamethrin in freshwater fish Nile tilapia, Oreochromis niloticus: impact on lipid peroxidation and oxidative stress. Environmental Science Pollution Research, 22, 3023-3031. http://dx.doi.org/10.1007/s11356-014-3578-0

Abdel-Tawab, M., & Ahmed, H. (2009). Live Spirulina (Arthrospira platensis) as a growth and immunity promoter for Nile tilapia, Oreochromis niloticus (L.), challenged with pathogenic Aeromonas hydrophila. Aquaculture Research, 40(9), 1037-1046. http://dx.doi. org/10.1111/j.1365-2109.2009.02195.x

Abdulrahman, N.M., & Ameen, H.J.H. (2014). Replacement of fishmeal with microalgae Spirulina on common carp weight gain, meat and sensitive composition and survival. Pakistan Journal of Nutrition, 13, 93-98. http://dx.doi.org/10.3923/pjn.2014.93.98

Abu-Elala, N.M., Galal, M.K., Abd-Elsalam, R.M., MoheyElsaeed, O., & Ragaa, N.M. (2016). Effects of dietary supplementation of Spirulina platensis and Garlic on the growth performance and expression levels of immune-related genes in Nile tilapia (Oreochromis niloticus). Journal of Aquaculture Research and Development, 7(7), 433-442. http://dx.doi.org/10.4172/2155-9546.1000433

Acharya, G., & Mohanty, P.K. (2012). Haematological and serum biochemical parameters in different sexes of walking cat fish, Clarias batrachus (Linnaeus, 1758). International Journal of Science and Research, 3(8), 1914-1917.

Ahmadzade-Nia, Y., Naser Adl, K., Hezave, S.G., Hejazi, M.A., Hassanpour, S., Chaichisemsari, M., & Riyazi, S.R. (2011). Effect of replacing different levels of Soybean meal with Spirulina on performance in Rainbow Trout. Annals of Biological Research, 2(3), 374-379.

Ali, S.K., & Saleh, A.M. (2012). Spirulina – an overview. International Journal of Pharmacy and Pharmaceutical Sciences, 4(3), 9-15.

Baker, M.E. (2002). Albumin, steroid hormones and the origin of vertebrates. Journal of Endocrinology, 175, 121-127.

Belal, E.B., Khalafalla, M.M.E., & El-Hais, A.M.A. (2012). Use of spirulina (Arthrospira fusiformis) for promoting growth of Nile Tilapia fingerlings. African Journal of Microbiology Research, 6(35), 6423-6431. http://dx.doi.org/10.5897/AMJR12.288

Belay, A. (2002). The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. The Journal of the American Nutraceutical Association, 5(2), 27-31.

Bermejo, P., Pinero, E., & Villar, A. (2008). Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chemistry, 110, 436-445. http://dx.doi. org/10.1016/j.foodchem.2008.02.021.

Beresto, V., 2001. Our experience in spirulina feeding to minks in the reproduction period. Scientifur 25, 11-15.

Blavan, P.S., Devi, V.G., Shanti, R., Radhakrishnan, S., & Poongodi, R. (2010). Basic biochemical constituent and profiles of amino acids in the post larvae of Macrobrachium rosenbergii fed with Spirulina and yeast enriched Artemia. Journal of Scientific Research, 2(3), 539-549. http://dx.doi. org/10.3329/jsr.v2i3.3663

Chairlan & Lestari, E. (2011). Manual of Basic Techniques for a Health Laboratory. 2 nd Eds. Publisher of medical books, EGC, 373 pp. (in Indonesian).

Chen, Y.E., Jin, S., & Wang, G.L. (2005). Study on blood physiological and biochemical indices of Vibrio alginilyticus disease of Lateolabrax japonicas. Journal Oceanography in Taiwan Strait, 24, 104-108.

Cnaani, A., Tinman, S., Avidar, Y., Ron, M., & Hulata, G. (2004). Comparative study of biochemical parameters in response to stress in Oreochromis aureus, O. mossambicus and two strains of O. niloticus. Aquaculture Research, 35, 1434-1440. http://dx.doi.org/10.1111/j.1365-21092004.01167.x

Demir, B.S., & Tukel, S.S. (2010). Purification and characterization of lipase from Spirulina platensis. Journal of Molecular Catalysis B: Enzymatic, 64, 123-128. http://dx.doi.org/10.1016/j.molcatb.2009.09.011

Dernekbasi, S., Una, H., Karayucel, I., & Aral, O. (2010). Effect of dietary supplementation of different rates of Spirulina (Spirulina platensis) on growth, and feed conversion in guppy (Poecilia reticulate peters, 1860). Journal of Animal Veterinary Advances, 9(9), 1395-1399.

De Pedro, N., Guijarro, A.E., Lopez-Patino, M.A., MarinesAlvares, R., & Delgado, M. (2005). Daily and seasonal variation in haematological and blood biochemical parameters in tench Tinca tinca. Aquaculture Research, 36, 85-96. http://dx.doi. org/10.1111/j.1365-21092005.01338.x

Djajasewaka, H. (2004). Effect of different protein levels in feed gurami parent (Osphronemus gouramy) on egg production and quality. Biosfera, 21(3), 84-88.

El-Sheekh, M., El-Shourbagy, I., Salaby, S., & Hosny, S. (2014). Effect of feeding Arthrospira platensis (Spirulina) on growth and carcas composition of Hybrid Red Tilapia (Oreochromis niloticus x Oreochromis mossambicus). Turkish Journal of Fisheries and Aquatic Sciences, 14, 471-478. http://dx.doi.org/10.4194/1303-2712-v14_2_18

Gutierrez-Salmean, G., Fabila-Castillo, L., & ChamorroCevallos, G. (2015). Nutritional and toxicological aspects of Spirulina (Arthrospira). Nutricion Hospitalaria, 32(1), 34-40. http://dx.doi.org/10.3305/nh.2015.32.1.9001

Ibrahem, M.D., Mohamed, M.F., & Ibrahim, M.A. (2013). The role of Spirulina platensis (Arthrospira platensis) in growth and immunity of Nile Tilapia (Oreochromis niloticus) and its resistance to bacterial infection. Journal of Agricultural Science, 5(6), 109–117. http://dx.doi.org/10.5539/jas.v5n6p109

Jaime-Ceballos, B.J., Hernandes-Llamas, A., GarciaGalano, T., & Villarreal, H. (2006). Substitution of Chaetoceros muelleri by Spirulina platensis meal in diets for Litopenaeus schmitti larvae. Aquaculture, 260, 215-220. http://dx.doi.org/10.1016/j.aquaculture.2006.06.002

Jana, A., Saroch, J.D., & Borana, K. (2014). Effect of Spirulina as a feed supplement on survival and growth of Pangasius sutchi. International Journal of Fisheries and Aquatic Studies, 1(5), 77-79.

Kamble, S.P., Gaikar, R.B., Padalia, R.B., & Shinde, K.D. (2013). Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity. Journal of Applied Pharmaceutical Science, 3(08), 149-153. http://dx.doi.org/10.7324/JAPS.2013.3826

Karakatsouli, N. (2012). An overview of the use of fatty acids in the fish farming research during the last decade, with particular emphasis on fish quality. Journal of World Aquaculture Society, 43, 291-320. http://dx.doi.org/10.1111/j.1749-7345.2012.00565.x

Kim, S.S., Rahimnejad, S., Kim K.W., & Lee, K.J. (2013). Partial replacement of fishmeal with Spirulina pacifica in diets for parrot fish (Oplegnathus fasciatus). Turkish Journal of Fisheries and Aquatic Sciences, 13, 197-204. http://dx.doi.org/10.4194/1303-2712-v13_2_01

Kim, S.S., Rahimnejad, S., Kim, K.W., Lee, B.J., & Lee, K.J. (2013). Effect of dietary supplementation of Spirulina and Quercetin on growth, innate immune responses, disease resistance against Edwardsiella tarda, and dietary antioxidant capacity in the juvenile olive flounder Paralichthys olivaceus. Fisheries and Aquatic Sciences, 16(1), 7-14. http://dx.doi.org/10.5657/FAS.2013.0007

Liu, Q., Huang, Y., Zhang, R., Cai, T. and Cai, Y. (2016). Medical Application of Spirulina platensis Derived CPhycocyanin. Hindawi Publishing Corporation, Evidence-Based Complementary and Alternative Medicine, 2016, 14 p. http://dx.doi.org/10.1155/2016/7803846

Moreira, L.M., Behling, B., Rodrigues, R.S., Costa, J.A.V., & Souza-Soares, L.A. (2013). Spirulina as a protein source in the nutritional recovery of wistar Rats. Brazilian Archives of Biology Technology, 56(3), 447- 456. http://dx.doi.org/10.1590/S1516- 89132013000300013

Moreira, L.M., Ribeiro, A.C., Duarte, F.A., de Morais, M.G., & Souza-Soares, L.A. (2013). Spirulina platensis biomass cultivated in Southern Brazil as a source of essential minerals and other nutrients. African Journal of Food Science, 7(12), 451-455. http://dx.doi.org/10.5897/AJFS2013.1082

Mutlu, E., Aydin, S., & Kutlu, B. (2015). Alterations of growth performance and blood chemistry in Nile tilapia (Oreochromis nuoticus) affected b copper sulfate in long-term exposure. Turkish Journal of Fisheries and Aquatic Sciences, 15, 481-488. http://dx.doi.org/10.4194/1303-2712-v15_2_35

Patocka, G., Cervenkova, R., Narine, S., & Jelen, P. (2006). Rheological behavior of dairy products as affected by soluble whey protein isolate. Internatonal Dairy Journal, 16, 399-405. http://dx.doi.org/10.1016/j.idairyj.2005.05.010

Phang, S.M., Miah, M.S., Yeoh, B.G., & Hashim, M.A. (2000). Spirulina cultivation in digested sago starch factory wastewater. Journal Applied Phycology, 12(3), 395-400. http://dx.doi.org/ 10.1023/A:1008157731731

Pogaku, R., Seng, C.E., Boonbeng, L., & Kallu, U.R. (2007). Whey protein isolate-starch system - A critical review. International Journal of Food Engineering, 3(6), 1-28. http://dx.doi.org/ 10.2202/1556- 3758.1164

Prasanna, R., Sood, A., Suresh, A., Nayak, S., & Kaushik, B. (2007). Potentials and applications of algal pigments in biology and industry. Acta Botanica Hungaria, 9 (1-2), 131-156. http://dx.doi.org/ 10.1556/ABot.49.2007.1-2.14

Preeti, M., & Seema, G. (2014). Modulatory effect of eclipta alba on biochemical parameters of catfish, Clarias batrachus. World Journal of Pharmaceutical Research, 3(4), 1223-1233.

Promya, J., & Chitmanat, C. (2011). The effects of Spirulina platensis and Cladophora algae on the growth performance, meat quality and immunity stimulating capacity of the African sharptooth catfish (Clarias gariepinus). International Journal of Agriculture and Biology, 13, 77-82.

Rehulka, J., Minarik, B., & Rehulkova, E. (2004). Red blood cell indices of rainbow trout Oncorhynchus mykiss (Walbaum) in aquaculture. Aquaculture Research, 35, 529-546. http://dx.doi.org/10.1111/j.1365-2109.2004.01035x

Saranraj, P., & Sivasakthi, S. (2014). Spirulina platensis – Food for future: A Review. Asian Journal of Pharmaceutical Science and Technology, 4(1), 26-33. Simanjuntak, S.B.I., Dana, D., Wirawidjaja, A.M., &

Supriyadi, H. (2002). The effectiveness of Spirulina as immunostimulants on patin jambal fish (Pangasius djambal Bleeker). Journal Biologi Indonesia, III(3), 209-218 (in Indonesian).

Simanjuntak, S.B.I., Dana, D., Wirawidjaja, A.M., & Supriyadi, H. (2003). Histopathologic spleen and kidney organ of patin jambal fish (Pangasius djambal Bleeker) giving due Spirulina in a discontinuous manner feed. Biosfera, 20(2), 62-66 (in Indonesian).

Simanjuntak, S.B.I., Yuwono, E., & Rachmawati, F.N. (2006). Effect of supplementation Spirulina in feed against hematological nilem fish (Osteochilus hasselti C.V.). Journal of Rural Development, 6(2), 84-88 (in Indonesian).

Simanjuntak, S.B.I., Moeljopawiro, S., Artama, W.T., & Wahyuono, S. (2011a). The effect of Spirulina platensis extract on humoral immune of mice after tachyzoite test. Biota, 16(1), 70-79 (in Indonesian).

Simanjuntak, S.B.I., Moeljopawiro, S., Artama, W.T., & Wahyuono, S. (2011b). The responses of immunoglobulin-G and immunoglobulin-M on mice administered with methanol extracts of blue green algae and challenged with tachyzoit. Journal Veteriner, 12(4), 281-287 (in Indonesian)

Simanjuntak, S.B.I., Soedibya, P.H.T., & Wibowo, E.S. (2014). Growth performance of gurami (Osphronemus gouramy Lac.) the given phytoplankton Spirulina platensis and Chlorella vulgaris, (pp. 157-165). Proceeding of the National Seminar on "Accelerating Rural Community Self-Reliance Through empowerment and Technological Innovation.” 20-21 November 2014, 2309 pp. ISBN 978-602-1643-13-6. (in Indonesian).

Simanjuntak, S.B.I., Wibowo, E.S. & Indarmawan. (2015). Variations long giving Spirulina platensis supplementation on growth gurami (Osphronemus gouramy Lac.), (pp. 546-554). Proceeding of the National Seminar on "Development of Rural Resources and Local Wisdom V.” 19-20 November 2015, 2439 pp. ISBN 978-602-1643-14-3. (in Indonesian).

Simpore, J., Pignatelli, S., & Musumeei S. (2007). The effect of Spiruline on the immune functions of HIVinfected undernourished children. The Journal of Infection in Developing Countries, 1(2), 112-117.

Sirakov, I., Velichkova, K., & Nikolov, G. (2012). The effect of algae meal (Spirulina) on the growth performance and carcass parameters of rainbow trout (Oncorhynchus mykiss). Journal Biological Science Biotechnology, 151-156.

Sitanggang, M. & Sarwono, B. (2001). Cultivation of gurami. Penebar Swadaya, Jakarta, 71 pp. (in Indonesian).

Svobodova, Z., Kroupova, H., Modra, H., Flajshans, M., Randak, T., Savina, L.V., & Gela, D. (2008). Haematological profile of common carp spawners of various breeds. Journal of Applied Ichthyology, 24(1), 55-59. http://dx.doi.org/10.1111/j.1439-0426.2007.01019.x

Teimouri. M., Amirkolaie, A.K., & Yeganeh, S. (2013). Effect of Spirulina platensis meal as a feed supplement on growth performance and pigmentation of rainbow trout (Oncorhynchus mykiss). World Journal of Fish and Marine Sciences, 5(2), 194-202. http://dx.doi.org/10.5829/idosi.wjfms.2013.05.02.722 3

Ungsethaphand, T., Peerapornpisal, Y., Whangchai, N., & Sardsud, U. (2010). Effect of feeding Spirulina platensis on growth and carcass composition of hybrid red tilapia (Oreochromis mossambicus x Oreochromis niloticus). Maejo International Journal of Science and Technology, 4(2), 331-336.

Vonshak, A., & Tomaselli, L. (2002). Arthrospira (Spirulina): systematics and ecophysiology. In: B.A. Whitton & M. Potts (Eds), The ecology of cyanobacteria. Their diversity in time and space. Netherlands (pp. 505-522). Kluwer Academic Publishers, 669 pp.

Wang, L., Pan, B., Sheng, J., Xu, J., & Hu, Q. (2007). Antioxidant activity of Spirulina platensis extracts by supercritical carbon dioxide extraction. Food Chemistry, 105, 36-41. http://dx.doi.org/10.1016/j.foodchem.2007.03.054

Zeinab, A.K., Aly, M.S., Faiza, A.K., & Fatma, E.M. (2015). Effect of Spirulina platensis and Lactobacillus rhamnosa on growth and biochemical performance of Nile Tilapia (Orechromis niloticus) fingerlings. International Journal of Current Microbiology and Applied Sciences, 4(4), 747-763.