Nihan ARIĞ, CÜNEYT SUZER, Alize GÖKVARDAR, Fatih BAŞARAN, DENİZ ÇOBAN, ŞÜKRÜ YILDIRIM, H. Okan KAMACI, MUAMMER KÜRŞAT FIRAT, ŞAHİN SAKA

Effects of probiotic (Bacillus sp.) supplementation during larval development of Gilthead Sea Bream (Sparus aurata, L.)

Bu çalışmada, çipura larva yetiştiriciliğinde 40. güne kadar ticari bir ürün olan probiotik bakteri (Bacillus sp.) eklenmesinin larvalarda büyüme parametreleri ve sindirim enzimleri (alkalin ve asit proteaz) üzerine olan etkileri incelenmiştir. Bütün denemeler 3 tekrarlı yürütülmüş ve probiotik eklenmesi ağız açılımı ile birlikte eş zamanlı olarak yumurtadan çıktıktan sonraki 3. günden itibaren uygulanmıştır. Deneme grubunda, probiotik bakteriler 20. güne kadar rotifer kültürüne ve tank ortamına eklenmiştir. Bunun yanında, kontrol grubuna herhangi bir uygulama yapılmamıştır. Deneme grubunda, alkalin proteaz spesifik aktivitesi kontrol grubuna göre önemli derecede yüksek bulunmuştur (P0,05). Ek olarak, rotifer kültürüne ve tank ortamına eklenen çipura larvalarının yaşama oranı (%8,5 daha yüksek) ve spesifik büyüme oranı (%0.7 daha yüksek) değerleri kontrol grubuna göre önemsiz farklılıklar göstermiştir (P>0,05). Sonuç olarak, probiotiklerin canlı yem kültürü ve tank ortamına eklenmesi alkalin ve asit proteaz aktivitesini önemli oranda artırmış, bu yüzden probiotiklerin bu yöntemle uygulanmasının büyüme parametreleri ve besinsel durumları açısından daha etkili olduğu sonucuna varılmıştır.

Probiotik (Bacillus sp.) eklenmesinin çipura (sparus aurata, L.) larvalarında gelişime olan etkileri

In this study, the influence of commercial probiotic, Bacillus spp., supplementation was investigated on growth parameters and digestive protease (alkaline and acid proteases) activities in gilthead sea bream, Sparus aurata, during larval development until on day 40. All experiments were triplicated and designed in administration of probiotic from 3 days after hatching concurrently with starting of exogenous feeding. In the experimental group, probiotic was added to rotifer and tank water until 20 days after hatching. Also, control group did not receive probiotic. In experimental group, the specific activities of alkaline protease was significantly higher (P<0.05) in larvae compared to control group. Acid protease activity was not effected by probiotic supplementation however, it was relatively higher in experimental group than the control (P>0.05). Additionally, S. aurata larvae that had probiotic administered by rotifer with water demonstrated insignificant (P>0.05) increases in both survival (8.5% higher) and specific growth rate (0.7% higher) as compared to controls. As a result, supplementation of probiotic to live food with water could significantly increase the specific activities of alkaline and acid protease and therefore, administration of probiotics by this method would be effective in terms of husbandry parameters and nutritional condition in larvae of S. aurata.

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Alarcón, F.J., Díaz, M., Moyano, F.J. and Abellán, E. 1998. Characterization and funtional properties of digestive proteases in two sparids; gilthead seabream (Sparus aurata) and common dentex (Dentex dentex). Fish Physiol. Biochem., 19: 257267. doi: 10.1023/A:1007717708491
Anson, M.L. 1938. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. Journal of General Physiology, 22: 7989.
Avella, M.A., Gioacchini, G., Decamp, O., Makridis, P., Bracciatelli, C. and Carnevali, O. 2010. Application of multispecies of Bacillus in sea bream larviculture. Aquaculture, 305: 1219. doi: 10.1016/j.aquaculture.2010.03.029,
Bairagi, A., Ghosh, K. S., Sen, S.K. and Ray, A.K. 2002. Enzyme producing bacterial flora isolated from fish digestive tracts. Aquaculture International, 10: 109 121. doi: 10.1023/A:1021355406412
Balcázar, J.L., Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D. and Múzquiz, J.L. 2006. The role of probiotics in aquaculture. Veterinary Microbiology, 114: 173186. doi: 10.1016/j.vetmic.2006.01.009
Bradford, M.M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem., 72: 248254.
DíazRosales, P., Salinas, I., Rodríguez, A., Cuesta, A., Chabrillón, M., Balebona, M.C., Morinigo, M.Á., Esteban, M.Á. and Meseguer, J. 2006. Gilthead seabream (Sparus aurata, L.) innate immune response after dietary administration of heatinactivated potential probiotics. Fish and Shellfish Immunology, 20: 482492. doi: 10.1016/j.fsi.2005.06.007
Dimitroglou, A., Merrifield, D.L., Carnevali, O., Picchietti, S., Avella, M., Daniels, C., Guroy, D. and Davies, S.J., 2011. Microbial manipulations to improve fish health and production A Mediterranean perspective. Fish and Shellfish Immunology, 30: 116. doi: 10.1016/j.fsi.2010.08.009
Eddy, S.D. and Jones, S.H. 2002. Microbiology of summer flounder Paralichthys dentatus fingerling production at a marine fish hatchery. Aquaculture, 211: 928. doi: 10.1016/S0044-8486(01)00882-1
ElHaroun, E.R., Goda, AS, A.M. and Chowdury, M.A.K. ® 2006. Effect of dietary probiotic Biogensupplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.). Aquaculture Research, 37: 14731480. doi: 10.1111/j.1365- 2109.2006.01584.x
Gatesoupe, F.J. 1999. The use of probiotics in aquaculture. Aquaculture, 180: 147165. doi: 10.1016/S0044- 8486(99)00187-8
Gatesoupe, F.J. 2002. Probiotic and formaldehyde treatments of Artemia nauplii as food for larval pollack, Pollachius pollachius. Aquaculture, 212: 347360. doi: 10.1016/S0044-8486(02)00138-2
Gatesoupe, F.J. 2007. Live yeasts in the gut: Natural occurrence, dietary introduction, and their effects on fish health and development. Aquaculture, 267: 20 30. doi: 10.1016/j.aquaculture.2007.01.005
Ghosh, K., Sen, S.K. and Ray, A.K. 2003. Supplementation of an isolated fish gut bacterium, Bacillus circulans, in formulated diets for rohu, Labeo rohita, fingerlings. Israeli J. Aquacult.Bamidgeh, 55: 1321.
Hidalgo, M.C., Skalli, A., Abellán, E., Arizcun, M. and Cardenete, G. 2006. Dietary intake of probiotics and maslinic acid in juvenile dentex (Dentex dentex L.): effects on growth performance, survival and liver proteolytic activities. Aquaculture Nutrition, 12: 256 266. doi: 10.1111/j.1365-2095.2006.00408.x
Irene, S., Alberto, C., Ángeles, M.E. and José, M. 2005. Dietary administration of Lactobacillus delbrüeckii and Bacillus subtilis, used alone or combined, on cellular innate immune responses. Fish and Shellfish Immunology, 19: 6777. doi: 10.1016/j.fsi.2004.11.007
Iribarren, D., Dagá, P., Moreira, M.T. and Feijoo, G., 2012. Potential environmental effects of probiotics used in aquaculture. Aquaculture International, 20: 779789. doi: 10.1007/s10499-012-9502-z
Kolkovski, S. 2001. Digestive enzymes in fish larvae and juvenilesimplications and application to formulated diets. Aquaculture, 200: 181201. doi: 10.1016/S0044-8486(01)00700-1
Li, P., Burr, G.S., Goff, J., Whiteman, K.W., Davis, K.B., Vega, R.R., Neill, W.H. and Gatlin III, D.M. 2005. A preliminary study on the effects of dietary supplementation of brewers yeast and nucleotides, singularly or in combination, on juvenile red drum (Sciaenops ocellatus). Aquaculture Research, 36: 11201127. doi: 10.1111/j.1365-2109.2005.01333.x
Mohapatra, S., Chakraborty, T., Prusty, A. K., Das, P., Paniprasad, K. and Mohanta, K. N. 2012. Use of different microbial probiotics in the diet of rohu, Labeo rohita fingerlings: effects on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal microflora. Aquaculture Nutrition, 18: 111. doi: 10.1111/j.1365-2095.2011.00866.x
Panigrahi, A., Kiron, V., Puangkaew, J., Kobayashi, T., Satoh, S. and Sugita, H. 2005. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Onchorhynchus mykiss. Aquaculture, 243: 241254. doi: 10.1016/j.aquaculture.2004.09.032
Planas, M., Pérez-Lorenzo, M., Hjelm, M., Gram, L., Fiksdal, U., Bergh, Ø. and Pintado, J. 2006. Probiotic effect in vivo of Rosebacter strain 27-4 against Vibrio (Listonella) anguillarum infections in turbot (Scophthalmus maximus L.) larvae. Aquaculture, 255: 323333. doi: 10.1016/j.aquaculture.2005.11.039
Robertson, P.A.W., ODowd, C., Burrels, C., Williams, P. and Austin, B. 2000. Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum). Aquaculture, 185 : 235243. doi: 10.1016/S0044- 8486(99)00349-X
Salinas, I., Cuesta, A., Ángeles Esteban, M. and Meseguer, J. 2005. Dietary administration of Lactobacillus delbrüeckii and Bacillus subtilis, single or combined, on gilthead seabream cellular innate immune responses. Fish and Shellfish Immunology, 19: 6777. doi: 10.1016/j.fsi.2004.11.007
Salinas, I., Díaz-Rosales, P., Cuesta, A., Meseguer, J., Chabrillón, M., Morinigo, M.Á. and Ángeles Esteban, M. 2006. Effect of heat-inactivated fish and non-fish derived probiotics on the innate immune parameters of a teleost fish (Sparus aurata L.). Veterinary Immunology and Immunopathology 111, 279286. doi: 10.1016/j.vetimm.2006.01.020
Suzer, C., Çoban, D., Kamacı, H.O., Saka, S., Fırat, K., Otgucuoğlu, Ö. and Küçüksarı, H. 2008. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: effects on growth performance and digestive enzyme activities. Aquaculture, 280: 140145. doi: 10.1016/j.aquaculture.2008.04.020
Taoka, Y., Maeda, H., Jo, J.Y., Jeon, M.J., Bai, S.C., Lee, W.J., Yuge, K. and Koshio, S. 2006. Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system. Fisheries Science, 72: 310321. doi: 10.1111/j.1444-2906.2006.01152.x
Thompson, F.L., Abreu, P.C. and Cavalli, R. 1999. The use of microorganisms as food source for Penaeus paulensis larvae. Aquaculture, 174: 139153. doi: 10.1016/S0044-8486(98)00511-0
Verschuere, L., Rombaut, G., Sorgeloos, P. and Verstraete, W. 2000. Probiotics bacteria as biological control agents in aquaculture. Microbiol. Mol. Biol. Rev., 64: 655671.
Villamil, L., Figueras, A., Planas, M. and Novoa, B. 2003. Control of Vibrio alginolyticus in Artemia cultura by treatment with bacterial probiotics. Aquaculture, 219: 4356. doi: 10.1016/S0044-8486(02)00515-X
Wang, Y.B. and Xu, Z.R. 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Anim. Feed Sci. Technol., 127: 283292. doi: 10.1016/j.anifeedsci.2005.09.003
Wang, Y.B. 2007. Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture, 269: 259 264. doi: 10.1016/j.aquaculture.2007.05.035
Wang, Y.B, Li, J.R. and Lin, J. 2008. Probiotics in aquaculture: Challenges and outlook. Aquaculture, 281: 14. doi: 10.1016/j.aquaculture.2008.06.002
Wu, Z.X., Feng, X., Xie, L.L., Peng, X.Y., Yuan, J. and Chen, X.X. 2012. Effect of Bacillus subtilis Ch9 for grass carp, Ctenopharyngodon idella (Valenciennes, 1844), on growth performance, digestive enzyme activities and intestinal microflora. J. Appl. Ichthyol. 28: 721727. doi: 10.1111/j.1439-0426.2012.01968.x
Ziaei-Nejad, S., Rezaei, M.H., Takami, G.A., Lovett, D.L., Mirvaghefi, A.R. and Shakouri, M. 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252: 516524. doi: 10.1016/j.aquaculture.2005.07.021
Zhou, X., Wang, Y. and Li, W. 2009. Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities. Aquaculture, 287: 349353. doi: 10.1016/j.aquaculture.2008.10.046