Effects of environmental contaminants and natural substances on vitellogenesis in Tilapia primary hepatocytes

Bazı kimyasalların ve bitki materyallerinin estrojenik etkinliği in vitro sistemi içinde vitellojenin (VTG) sentezi uyarımı dişi ve estradiol uygulanmış erkek Mozambik tilapyalardan, Oreochromis mossambicus, elde edilmiş birincil hepatosit kültüründe değerlendirilmiştir. Sırası ile endokrin bozucu kimyasallar (EDCs) olarak; nonylphenol (NP), bisphenol-A (BP), octylphenol (OP), hexachlorocyclohexane (HCH) ve hepsinin kombinasyonu (ALL) ile ticari fitoestrojen olarak; Solgen 40®, SG40 ve doğal materyaller olarak bitki tohumlarının özütleri (çemen, FG; anason, AS; aspir, SF; keten, FS; rezene, FN; meyan kökü, LR; nar, PG; soya, SB) kullanılmıştır. İzole edilmiş hepatositler bu bileşimlere maruz bırakılmış ve onların estrojenik aktivitesi kontrol (etanol uygulanmış hepatosit) ile karşılaştırılmıştır. Hepatik VTG seviyeleri birincil hepatosit kültüründeki medyum içinde ELISA ile tespit edilmiştir. Test edilen bütün bileşimler dişi ve erkek tilapyaların hepatositlerinde VTG salgılanmasına neden olmuştur. Hepatik tepkinin indüklenmesi dişilerde EDCs’lerden HCH’ de ve bitki özütlerinden FG, AS, FN, LQ ve PG’ de ve erkeklerde de bütün EDCs’lerde ve bitkilerde de SG40 ve SB dışında kontrolden farklı bulunmamıştır. Bu denemede kullanılan EDCs’ler ve bitki materyallerinin estrojenik etkisi estradiole göre oldukça azdır. Çevresel estrojenin varlığını tespit etmede VTG ölçümü için kullanılan tilapya hepatosit tahlili değerli bir araç özelliğini taşımaktadır.

Anahtar Kelimeler:

Oreochromis mossambicus, rezene, çemen otu, bitki bileşimi, aspir, soya fasulyesi, Foeniculum vulgare, Trigonella foenum-graecum, çevresel etki, keten, karaciğer hücreleri, östrojen, bitki özütü, bitkisel östrojenler, yumurta sarısı oluşumu, Carthamus tinctorius, glisin, Linum usitatissimum

Çevresel kirleticilerin ve doğal maddelerin Tilapya birincil hepatositlerinde vitellojenesize etkileri

The efficacy of estrogenic chemicals and plant materials were assessed to stimulate the vitellogenin (VTG) synthesis in an in vitro system based on primary cultures of hepatocytes from female and E2-primed male Mozambique tilapia, Oreochromis mossambicus. Isolated hepatoyctes treated with nonylphenol (NP), bisphenol-A (BP), octylphenol (OP), hexachlorocyclohexane (HCH) and the combination (ALL) as endocrine-disrupting chemicals (EDCs), Solgen 40®, SG40, as a commercial phytoestrogens and plant extracts (fenugreek, FG; aniseed, AS; safflower, SF; flaxseed, FS; fennel, FN; liqurice, LR; pomegranate, PG; soybean, SB) as natural substances, respectively, and their estrogenic activity were compared to control (ethanol treated hepatoyctes). Hepatic VTG levels in medium were determined by ELISA in the primary hepatocytes culture. All tested compounds induced secretion of VTG in hepatocytes of female as well as male tilapia. The induction of the hepatic response was not statistically different when each compounds was compared to control with HCH from EDCs and FG, AS, FN, LQ and PG from plant extracts in female and all cases of EDCs and plant extracts (except SG40 and SB) in male hepatoyctes. The EDCs and plant materials tested were considerably less potent than estradiol. Sensitive tilapia hepatoycte assay for measuring VTG are valuable tools for identifying the presence of environmental estrogens.

Keywords:

Oreochromis mossambicus, fennel, fenugreek, plant composition, safflower, soyabeans, Foeniculum vulgare, Trigonella foenum-graecum, environmental impact, flax, liver cells, oestrogens, plant extracts, plant oestrogens, vitellogenesis, Carthamus tinctorius, glycine, Linum usitatissimum,

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