A Survey of viral hemorrhagic septicemia (VHS) in Turkey

Türkiye'de viral hemorajik septisemi virüsünün (VHSV) varlığı son yıllarda rapor edilmiştir. Bu çalışmanın amacı 2007- 2009 yılları arasında, Karadeniz bölgesinde, tatlı su ve deniz balıklarında VHSV'nin varlığının araştırılmasıdır. Tarama bölgesinden 2009 yılının sonuna kadar 13 farklı deniz ve tatlı su balığı türünden toplam 5967 adet balık örneklenmiş, balık örneklerinden beş tanesi hem dokudan hem de CPE gösteren hücre kültürü süpernatantından, VHSV'nin glikoprotein geninin 587 bç'lik korunmuş bir bölgesine spesifik primerler kullanılarak yapılan PCR reaksiyonuyla pozitif bulunmuştur. Pozitif bulunan tüm örnekler yalnızca kalkan balıklarından alınmıştır. Bu beş izolatın nükleotit dizilimleri arasında %96 ila %98 homoloji olduğu görülmektedir. Yapılan filogenetik analizde tüm izolatların genogrup Ie içerisinde yer aldıkları tespit edilmiştir.

Anahtar Kelimeler:

Akdeniz, polimeraz zincir reaksiyonu, filogeni, nükleotit dizileri, karışık enfeksiyon, genler, epidemiyoloji, hastalık yaygınlığı, Türkiye, Karadeniz, hastalık tetkiki, hastalık bulaşması, epidemiyolojik araştırmalar, tatlısu balıkları, glikoprotein, deniz balıkları, viral proteinler

Türkiye'de viral hemorajik septisemi (VHS) Taraması

Existence of viral hemorrhagic septicemia virus (VHSV) has been recently reported in Turkey. A total of 5967 marine and freshwater fish were sampled to detect VHSV between 2007 and 2009 in the Black Sea region of Turkey. Of the 5967 fish, five fish tested positive by PCR reactions of tissue filtrates and supernatants of cell cultures showing CPE indicated by a specific 587 bp fragments from the glycoprotein (G) gene of VHSV. All the VHSV positive fish were turbots and none of the other fish species was VHSV positive. The nucleotide sequences among five isolates had shown 96% to 99% homology. In the phylogenetic study, all the new isolates were genogroup 1e. In this study, it was also discovered that an IPNV-VHSV mixed infected wild turbot.

Keywords:

Mediterranean Sea, polymerase chain reaction, phylogeny, nucleotide sequences, mixed infections, genes, epidemiology, disease prevalence, Turkey, Black Sea, disease surveys, disease transmission, epidemiological surveys, freshwater fishes, glycoproteins, marine fishes, viral proteins,

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