Mustafa TÜRE, Halil İbrahim HALİLOĞLU, Cemil ALTUNTAŞ, Halis BORAN, İlyas KUTLU

Comparison of experimental susceptibility of rainbow trout ( oncorhynchus mykiss), turbot ( psetta maxima), black sea trout ( salmo trutta labrax) and sea bass( dicentrarchus labrax) to lactococcus garvieae

Lactoco ccus garvieae birçok balık türünde hemorajik septisemiye neden olan önemli bir balık patojenidir. Bu çalışma, bazı önemli balık türlerinin Lactococcus garvieae ye karşı duyarlılıklarının belirlenmesi ve karşılaştırılması amacıyla yapılmıştır. Gökkuşağı alabalığı, Karadeniz alabalığı, kalkan balığı ve deniz levreği 30 gün boyunca farklı sıcaklık aralıklarında (12 -23°C) tutularak, alabalıklardan izole edilen L. garvieae (Lgper) ve ATCC 49156 suşları ile periton içi (i.p.) ve daldırma (imm.) metotları ile dene ysel enfeksiyona tabi tutulmuştur. Deneylerde, 1,7x10 5 - 2x10 6 cfu/balık aralığında bakteri kullanılmıştır. Deneylerin sonucunda, Lgper suşunun gökkuşağı alabalıklarında %98 ölüm oranı ile yüksek virülansa sahip olduğu görülmüştür. Düşük sıcaklıkta, L. garvieae ye karşı test edilen gökkuşağı ve Karadeniz alabalıklarında ise ölümler düşük seviyede görülmüştür (max. mortalite %16). Bu iki balık türünün L. garvieae ye karşı duyarlılıkları benzer düzeyde bulunmuştur (P > 0,05). Ayrıca, L. garvieae ile enfekte e dilen kalkan balığı ve deniz levreklerinde ölüme ya da herhangi bir klinik bulguya rastlanmamıştır. Bu çalışmanın sonuçları, su ürünleri yetiştiriciliği alanında muhtemel laktokokosiz salgınlarının önlenmesi için önemli bir kaynak oluşturma potansiyeline s ahiptir.

Gökkuşağı alabalığı (oncorhynchus mykiss), kalkan balığı (psetta maxima),karadeniz alabalığı ( salmo trutta labrax) ve deniz levreğinin ( dicentrarchus labrax) lactococcus garvieae bakterisine karşı deneysel duyarlılıklarının karşılaştırılması

Lactoco ccus garvieae is a major fish pathogen leads to haemorragic septicemia in many fish species. Present study was designed to determine and compare differences in susceptibilities of some important fish species to L. garvieae . Rainbow trout (Onco r hynchus mykiss ), Black Sea trout (Salmo trutta labrax; synonym, Salmo coruhensis ), turbot (Psetta maxima ), and sea bass (Dicentrarchus labrax) were kept at different temperature ranges (12 -23°C) for 30 days and challenged by intraperitoneal (i.p.) injection and immersion (imm.) with L. garvieae (Lgper), isolated from rainbow trout and ATCC49156 strain. Bacteria were ranged between 1.7x10 5 - 2x10 6 colonies forming unit (cfu) fish -1 in experiments. As a result of the experiments, Lgper strain had high virulence for rainb ow trout, with 98% mortality when challenged by i.p. injection. Rainbow trout and Black Sea trout tested at the lower temperature (1 2ºC) were less susceptible (max. mortality 16%) to L. garvieae . These two fish species had similar susceptibilities to L. ga rvieae (P>0.05). There was no mortality or any clinical signs when turbot and sea bass were challenged with L. garvieae . The results of the study generate important source in controlling of possible lactococcosis outbreaks in aquaculture industry.

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