Hayati ZENGİN, ÖKKEŞ YILMAZ, ERSİN DEMİR, ZEHRA GÖKÇE

Antioxidant Enzymatic Defences during Embryogenesis of Rainbow Trout Oncorhynchus mykiss (Walbaum 1792)

Bu çalışmada süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHpx), glutatyon redüktaz (GR) ve Glutatyon S-transferaz (GST) gibi antioksidan enzimlerin aktiviteleri gökkuşağı alabalığı Oncorhynchus mykiss (Walbaum 1792)'in döllenmemiş ve döllenmiş yumurtaları, 12, 22 ve 32 günlük embriyolarında araştırılmıştır. E vitamini, lipid peroksidasyon ürünleri ve yağ asidi düzeyleri de belirlenmiştir. O. mykiss'in döllenmiş yumurtalarında E vitamini düzeyinde önemli bir azalma bulunmuştur. Aksine malondialdehid (MDA) düzeyi döllenmiş yumurtalar ve 12 günlük embriyolarda çok yüksek bulunmuştur. Döllenmiş yumurtalar ve 12 günlük embriyoların PUFA içerikleri diğer embriyonik safhalardan düşük bulunmuştur. SOD, CAT, GSHpx, GR ve GST enzim aktivitelerinin O. mykiss'in yumurta ve embriyolarında ölçülebilir miktarda olduğu belirlenmiştir. O. mykiss'in döllenmiş yumurtalarında SOD, GR and GST aktivitelerinde çok önemli bir azalma belirlenirken, aynı safhada CAT aktivitesinde oldukça önemli bir artış tespit edilmiştir. GSHpx aktivitesi döllenmiş yumurtalarda ve yumurtaların açılmasından önce 32 günlük embriyolarda önemli bir artış göstermiştir. GST aktivitesi gözlenme safhasında bulunan 22 günlük embriyolarda önemli bir artış göstermiştir

Gökkuşağı Alabalığı Oncorhynchus mykiss(Walbaum 1792)'in Embriyonik Gelişimi Esnasında Enzimatik Antioksidan Savunma

In this study antioxidant enzymes activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHpx), glutathione reductase (GR) and glutathione S-transferase (GST) were investigated in unfertilized and fertilized eggs, 12, 22 and 32 day embryos of rainbow trout Oncorhynchus mykiss (Walbaum 1792). Vitamin E, lipid peroxidation products and fatty acids levels were also determined. A significant decrease in vitamin E level was found in fertilized eggs of O. mykiss. In contrast, the level of malondialdehyde (MDA) was very high in fertilised eggs and 12 day embryo. The PUFA contents in fertilized eggs and 12 day embryo were found to be lower than in the other embryonic stages. Measurable amounts of all the SOD, CAT, GSHpx, GR and GST enzymes activities were present in O. mykiss eggs and embryos. The most intense decrease was detected in the activities of SOD, GR and GST in fertilized eggs, whereas the most intense increase in the CAT activity was detected in the same stage of O. mykiss. GSHpx activity showed a significant increase in fertilized eggs and in 32 day embryos before hatching. GST activity showed the most significant increase in 22 day embryos of eyed eggs stage

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