Bilal AKBULUT, Eyüp ÇAKMAK, Osman Tolga ÖZEL, Nafiye DÜLGER

Effect of anaesthesia with clove oil and benzocaine on feed intake in Siberian sturgeon (Acipenser baerii Brandt, 1869)

Bu araştırmanın amacı, Sibirya mersin balıklarının (Acipenser baerii Brandt, 1869) bayıltılması için en uygun karanfil yağı ve benzokainin dozlarını belirlemek, anesteziye maruz kalma süresinin ayılma süresine etkisini incelemek ve bu bayıltıcıların anesteziden sonra yem alımına etkilerini karşılaştırmaktır. Ayrıca, anestezi işleminin bir parçası olarak bu iki bayıltıcının en uygun konsantrasyonlarında 96 dakika içinde yaşama suresi ve anesteziye maruz kalma suresi ile ayılma suresi arasındaki ilişki incelenmiştir. Çalışmada ortalama boyları 10,0±0,93 (7,2-12,0) cm ve ağırlıkları 4,1±0,95 (2,1-6,4) g olan yavru balıklar kullanılmıştır. Bu balıkların karanfil yağı ve benzokain ile 3 dakika içinde bayılmaları için etkili konsantrasyonlar (EC50) %95 güven sınırları ile sırasıyla 356 (330-381) mg/L ve 37 (33-40) mg/L olarak tahmin edilmiştir. Karanfil yağı ile bayıltılan balıkların ayılma suresi 6, 12, 24 ve 48 dakikalarda benzokainden daha uzun olmuştur (P

Sibirya mersini (Acipenser baerii Brandt, 1869) nde benzokain ve karanfil yağı kullanımının yem alımına etkisi

The aim of this study wasto determine the optimal dose and the time required to reach a stable level of anaesthesia using clove oil and benzocaine on Siberian sturgeon fry (Acipenser baerii Brandt, 1869) with a mean length of 10.0±0.93 (7.2-12.0) cm and weight of 4.1±0.95 (2.1-6.4) g, and to compare the effects of them on feed intake after anaesthesia. In addition, the survival time and relationship between exposure duration and recovery time in 96 min with the optimal concentrations of these two anaesthetics were examined as part of the anaesthesia procedure. Effective concentrations (EC50) were estimated to be 356 (330-381) mg/L of clove oil and 37 (33-40) mg/L of benzocaine for 3 minutes with 95% confidence limits. The recovery time with clove oil was longer than with benzocaine at 6, 12, 24 and 48 minutes (P<0.05). The mean lethal times (MLT50) were estimated as 37.2 (5.1-69.3) min for clove oil and 63.0 (6.1-120.0) min for benzocaine with 95% confidence limits. Feed intake in fish treated with clove oil and benzocaine occurred within ten minutes, but the effect of both anaesthetics on feed intake continued for 4 h. However, it was determined that the effect of clove oil on feed intake was less than that of benzocaine.

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