Farklı desensitizerlerin dentin tübüllerine etkisinin incelenmesi: in vitro taramalı elektron mikroskop analizi

Amaç: Bu in vitro çalışmanın amacı Siyanoakrilat (Periacryl) ve tetra-kalsiyum fosfat ve dikalsiyum fosfat içerikli desensitizerin (Teethmate) kombine ve tekli uygulamalarının kalıcılığının ve dentin tübüllerine etkisinin değerlendirilmesidir. Gereç ve Yöntemler: Gömülü 20 yaş dişlerinden 140 dentin preparatı elde edilmiştir. Preparatlar 7 gruba ayrılmıştır (n=20). Hiçbir uygulama yapılmayan preparatlar “K” kontrol, %6 sitrik asitte 1 dk. bekletilenler PS ( Periacryl ) TS (Teethmate ) PTS(kombine uygulama) ; 24 saat yapay tükürükte bekletilenler TY (Teethmate ) PY (Periacryl ) PTY (kombine uygulama ) olarak gruplanmıştır. Uygulama tamamlandığında preparatlar SEM cihazı kullanılarak morfolojik yapıları, açık dentin tübül alanları ve açık dentin tübül çapları açısından değerlendirilmiştir. Bulgular: Açık dentin tübüllerinin alanı kontrol grubuna kıyasla PS, PY, PTS ve PTY gruplarında azalmıştır (p>0,05). Numunelerdeki açık dentin tübül çap ortalamaları kontrol grubuna göre PS, PY, PTY, PTS ve TY gruplarında anlamlı derecede azalmıştır (p>0,05) . Sitrik asit uygulanan gruplarda istatistiksel olarak anlamlı bir farklılık yokken yapay tükürük uygulanan gruplarda açık dentin tübül çap ortalaması, PTY grubunda TY grubundan istatistiksel olarak anlamlı derecede küçüktür (p>0,05) . Sonuç: Periacryl dentin hassasiyet tedavisinde kullanılabilecek bir preparat olmakla birlikte kombine kullanımda Teethmate ’in kalıcılığını arttırmaktadır.

Anahtar Kelimeler:

SEM, Periacryl, dentin desensitizasyon

Evaluation of the effect of different desensitizers on dentin tubules: An in vitro scanning electron microscopy analysis

Aim: The aim of this in vitro study is to evaluate the persistence of the combined and single applications of cyanoacrylate (Periacryl) and tetra-calcium phosphate + dicalcium phosphate containing desensitizer (Teethmate) and their effect on dentin tubules. Material and Methods: 140 dentin samples were obtained from impacted third molars. The samples were divided into 7 groups (n = 20). Samples without any application named as K (control), samples were subjected to 6% citric acid solution for 1 min; PS (Periacryl) TS (Teethmate) PTS (combined application). Samples were immersed in artificial saliva for 24 hours named as; TY (Teethmate) PY (Periacryl) PTY (combined application). When the application was completed, the preparations were evaluated by scanning electron microscopy in terms of morphological structures, open dentin tubule areas and open dentin tubule diameters.Results: The area of open dentin tubules decreased in PS, PY, PTS and PTY groups compared to the control group (p> 0.05). The mean diameter of the open dentin tubule in the samples was significantly decreased in the PS, PY, PTY, PTS and TY groups compared to the control group (p> 0.05). While there was no statistically significant difference in citric acid treated groups, the mean dentin tubule diameter was significantly lower in the group with artificial saliva than the TY group in the PTY group (p> 0.05). Conclusion: Periacryl is a preparation that can be used in the treatment of dentin sensitivity, however, it increases the retention of Teethmate in combined use.

Keywords:

SEM, Periacryl, Dentin Desensitization,

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