Synthesis, spectroscopic characterization, and computational studies of 2-cyano-6-hydroxybenzothiazole: a key synthetic intermediate of firefly luciferin
Synthesis, spectroscopic characterization, and computational studies of 2-cyano-6-hydroxybenzothiazole: a key synthetic intermediate of firefly luciferin
Firefly luciferin is widely applied as a biotechnological tool for visualizing various biological processes invitro and in vivo. Chemically, 2-cyano-6-hydroxybenzothiazole, as a key synthetic intermediate of firefly luciferin, isobtained from 2-cyano-6-methoxybenzothiazole by changing the methoxy with a hydroxy group. However, this approachis costly and not suited for large-scale synthesis. Here we report cost-effective and efficient syntheses of 2-cyano-6-hydroxybenzothiazole through the catalytic Sandmeyer-type cyanation reaction. Our approach employs diazoniumtetrafluoroborate salt of 2-amino-6-hydroxybenzothiazole as a cyanation substrate. The cyanation reaction proceedsefficiently under mild conditions by using Cu(I)/Cu(II)/N,N,N',N'-tetramethylethylenediamine as a catalyst. In addition,computational studies of the 2-cyano-6-hydroxybenzothiazole structure were performed based on the density functionaltheory method. The theoretical parameters of the optimized geometry were derived from the B3LYP/6-311++G(d,p) method. Time-dependent density functional theory was applied to assign the electronic absorption bands observedexperimentally and the 1 H NMR chemical shifts were computed using the GIAO method. There was a significantrelationship between computational studies and experimental results.
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- 1. Prescher, J. A.; Contag, C. H. Curr. Opin. Chem. Biol. 2010, 14, 80-89.
- 2. Li, J.; Chen, L.; Du, L.; Li, M. Chem. Soc. Rev. 2013, 42, 662-676.
- 3. Contag, C. H.; Jenkins, D.; Contag, P. R.; Negrin, R. S. Neoplasia. 2000, 2, 41-52.
- 4. Smirnova, D. V.; Samsonova, J. V.; Ugarova, N. N. J. Photochem. Photobiol. 2016, 92, 158-165.
- 5. Kurihara, M.; Ohmuro-Matsuyama, Y.; Ayabe, K.; Yamashita, T.; Yamaji, H.; Ueda, H. Biotechnol. J. 2016, 11, 91-99.
- 6. Green, A. A.; McElroy, W. D. ffBiochim. Biophys. Acta 1956, 20, 170-176.
- 7. Bitler, B.; McElroy, W. D. Arch. Biochem. Biophys. 1957, 72, 358-368.
- 8. White, E. H.; McCapra, F.; Field, G. F. J. Am. Chem. Soc. 1963, 85, 337-343.
- 9. White, E. H.; Rapaport, E.; Seliger, H. H.; Hopkins, T. A. Bioorg. Chem. 1971, 1, 92-122.
- 10. Seto, S.; Ogura, K.; Nishiyama, Y. Bull. Chem. Soc. Jpn. 1963, 36, 331-333.
- 11. Toya, Y.; Takagi, M.; Nakata, H.; Suzuki, N.; Isobe, M.; Goto, T. Bull. Chem. Soc. Jpn. 1992, 65, 392-395.
- 12. Seliger, H. H.; McElroy, W. D. Biochem. Biophys. Res. Commun. 1952, 1, 21-24.
- 13. Seliger, H. H.; McElroy, W. D. Arch. Biochem. Biophys. 1960, 88, 136-141.
- 14. White, E. H.; McCapra. F.; Field, G. F. J. Am. Chem. Soc. 1963, 85, 337-343.
- 15. Hodgson, H. H. Chem. Rev. 1947, 40, 251-77.
- 16. Rao, C. N. R.; Vanitha, P. V.; Cheetham, A. K. Chem. Eur. J. 2003, 9, 828-836.
- 17. Xu, W.; Xu, Q.; Li, J. Org. Chem. Front. 2015, 2, 231-235.
- 18. Cygler, M.; Przybylska, M.; Elofson, R. M. Can. J. Chem. 1982, 60, 2852-2855.
- 19. Colleville, A. P.; Horan, R. A.; Tomkinson, N. C. Org. Process. Res. Dev. 2014, 18, 1128-1136.
- 20. Sigeev, A. S.; Beletskaya, I. P.; Petrovskii. P. V.; Peregudov, A, S. Russ. J. Org. Chem. 2012, 48, 1055-1058.
- 21. Frisch, M. J. E. A.; Trucks, G. W.; Schlegel, H. B.; Scuseria, G. E.; Robb, M. A.; Cheeseman, J. R.; Scalmani, G.; Barone, V.; Mennucci, B.; Petersson, G. et al. Gaussian 09, Revision A 02; Gaussian Inc.: Wallingford, CT, USA, 2009.
- 22. Becke, A. D.ff Phys. Rev. 1988, 38, 3098.
- 23. Roglans, A.; Pla-Quintana, A.; Moreno-Manas, M. Chem. Rev. 2006, 106, 4622-4643
- 24. Bonin, H.; Fouquet, E.; Felpin, F. X. Adv. Synth. Catal. 2011, 353, 3063-3084.
- 25. Doyle, M. P.; Bryker, W. J. J. Org. Chem. 1979, 44, 1572-1574
- 26. Shopsowitz, K.; Francesco, Lelj, F.; MacLachlan, M. J. J. Org. Chem. 2011, 76, 1285-1294.
- 27. Kosynkin, D. V.; Tour, J. M. Org. Lett. 2001, 3, 993-995.
- 28. Doyle, M. P.; Bryker, W. J. J. Org. Chem. 1979, 44, 1572-1574.
- 29. Colas, C.; Goeldner, M. Eur. J. Org. Chem. 1999, 6, 1357-1366.
- 30. Flood, D. T. Org. Synth. 1933, 13, 46-46.
- 31. Balz, G.; Schiemann, G. Eur. J. Inorg. Chem. 1927, 60, 1186-1190.
- 32. Roe, A. Org. React. 1949, 5, 193-228.
- 33. Utsugi, M.; Ozawa, H.; Toyofuku, E.; Hatsuda, M. Org. Process Res. Dev. 2014, 18, 693-698.
- 34. Schareina, T.; Zapf, A.; Beller, M. Tetrahedron Lett. 2005, 46, 2585-2588.
- 35. Hanson, P.; Rowell, S. C.; Taylor, A. B.; Walton, P. H.; Timms, A. W. J. Chem. Soc. Perkin. Trans. 2002, 2, 1126-1134.
- 36. Schareina, T.; Zapf, A.; Beller, M. Tetrahedron Lett. 2005, 46, 2585-2588.
- 37. Beletskaya, I. P.; Sigeev, A. S.; Peregudov, A. S.; Petrovskii, P. V. J. Organomet. Chem. 2004, 689, 3810
- 38. Schareina, T.; Zapf, A.; Mägerlein, W.; Müller, N.; Beller, M. Synlett 2007, 4, 555-558.
- 39. Cristau, H. J.; Ouali, A.; Spindler, J. F.; Taillefer, M. Chem. Eur. J. 2005, 11, 2483-2492.
- 40. Zanon, J.; Klapars, A.; Buchwald, S. L. J. Am. Chem. Soc. 2003, 125, 2890-2891.
- 41. Starks, C. M. J. Am. Chem. Soc. 1971, 93, 195-199.
- 42. Sandmeyer, T. Eur. J. Inorg. Chem. 1884, 17, 1633-1635.
- 43. Harbour, J. R.; Issler, S. L. J. Am. Chem. Soc. 1984, 104, 903-905. 44. Gillespie, R. J. J. Chem. Educ. 1963, 40, 295.
- 45. Gillespie, R. J. J. Chem. Educ. 1970, 47, 18
- 46. Lau, P. T.; Compf, T. E. J. Org. Chem. 1970, 35, 4103-4108.