Mahfuz ELMASTAŞ, İsa TELCİ, Hüseyin AKSİT, Ramazan ERENLER, Fatih GÜL, Ömer KAYIR, Ali Rıza TÜFEKÇİ, İbrahim DEMİRTAŞ

Quantification of flavonoids isolated from Mentha spicata in selected clones of Turkish mint landraces

Mint has been used extensively in traditional medicines, spices, and herbal teas throughout the world.Isolation of flavonoids from Mentha spicata and quantification of these compounds in selected clones of Turkish mintslandraces were achieved. M. spicata was dried in shade and then boiled in water. After filtration, the aqueous extractwas partitioned with ethyl acetate. The solvent was removed under reduced pressure to yield the extract, subjected tosilica gel column chromatography to isolate 5-demethyl sinensetin (1), hesperidin (2), didymin (3), and linarin (4), thestructures of which were elucidated by 1D NMR, 2D NMR, and LC-TOF/MS. The isolated compounds were employedas standard flavonoids to determine the quantitative variations in cultivated Turkish mint landraces belonging to Mentha× piperita L., M. spicata L., and M. × villosonervata Opiz by HPLC. The quantities of hesperidin (2), didymin (3), andlinarin (4) in M. spicata were found as 11.83, 3.85, and 42.21 mg/g dried plant weight (DW), respectively, while that of5-demethyl sinensetin (1) in M. villosonervata was 2.9 mg/g DW. Consisting of pharmaceutically valuable compounds,M. spicata and cultivated Turkish mint landraces could be a source of 5-demethyl sinensetin (1), hesperidin (2), didymin(3), and linarin (4).

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