Quasielastic light scattering studies of polypeptides: evidence for chain extension in solution
Dynamic light scattering and viscosimetric methods were used to study polypeptides including polyleucine, poly (g-benzyl-L-glutamate), poly (a-L-glutamic acid), and polyproline II. Chain extension showing rod-like and flexible conformations occurred in poly(g -benzyl-L-glutamate) and polyproline II solutions as these solutions were diluted in glacial acetic acid or in a mixed solvent of acetic acid and dichloromethane. The elongation of chains in poly(g -benzyl-L-glutamate) and polyproline II solutions was studied by dynamic light scattering and viscosimetric methods. This nonenzymatic growth of peptide chains provides data on primordial synthesis of protein molecules in the absence of cell machinery and for this reason these reactions may yield valuable data for further studies.
Quasielastic light scattering studies of polypeptides: evidence for chain extension in solution
Dynamic light scattering and viscosimetric methods were used to study polypeptides including polyleucine, poly (g-benzyl-L-glutamate), poly (a-L-glutamic acid), and polyproline II. Chain extension showing rod-like and flexible conformations occurred in poly(g -benzyl-L-glutamate) and polyproline II solutions as these solutions were diluted in glacial acetic acid or in a mixed solvent of acetic acid and dichloromethane. The elongation of chains in poly(g -benzyl-L-glutamate) and polyproline II solutions was studied by dynamic light scattering and viscosimetric methods. This nonenzymatic growth of peptide chains provides data on primordial synthesis of protein molecules in the absence of cell machinery and for this reason these reactions may yield valuable data for further studies.
___
- Baysal, B. M.; Karasz, F. E. Macromol. Theor. Simul. 2003, 12, 627–646.
- Higuchi, M.; Inoue, T.; Miyoshi, H.; Kawaguchi, M. Langmuir 2003, 21, 11462–11474.
- Baars, S.; Drauz, K. H.; Krimmer, H. P.; Roberts, H. P. S.; Sander, J.; Skidmore, J.; Zanardi, G. Org. Process Res. Dev. 2003, 7, 509–514.
- Carrea, G.; Colonna, S.; Kelly, D. R.; Lazcano, A.; Ottolina, G.; Roberts, S. Trends Biotechnol. 2005, 23, 507–513. MacCallum, J. L.; Moghaddam, M. S.; Chan, H. S.; Tieleman, D. P. Proc. Natl. Acad. Sci. 2007, 104, 6206–6220. Blout, E. R.; Karlson, R. H .J. Am. Chem. Soc. 1956, 78, 941–946.
- Doty, P.; Bradbury, P. J. H.; Holtzer, A. M. J. Am. Chem. Soc. 1956, 78, 947–951.
- Doty, P.; Yang, J. T. J. Am. Chem. Soc. 1956, 78, 497–511.
- Zimm, B. H.; Doty, P.; Iso, P. K. Proc. Natl. Acad. Sci. 1959, 45, 1601–1614.
- Fujita, H.; Teramoto, A.; Okita, K.; Yamashita, T; Ikeda, S. Biopolymers 1966, 4, 769–784.
- Fujita, H.; Teramoto, A; Okita, K; Yamashita, T; Ikeda, S. Biopolymers 1966, 4, 781–795.
- Blout, E. R. Proc. Natl. Acad. Sci. 1961, 13, 123–136.
- Doty, P.; Lundberg, R. D. Proc. Natl. Acad. Sci. 1957, 43, 213–225.
- Elliott, A.; Handby, W. E.; Malcolm, B. R. Nature 1956, 178, 1170–1183.
- Teramoto, A.; Nakagawa, K., Fujita, H. J. Chem. Phys. 1967, 46, 4197–4202.
- Iwata, K. Biopolymers 1980, 19, 125–136.
- Matsumoto, T; Teramoto, A. Biopolymers 1974, 13, 1347–1359.
- Steinberg, I. Z.; Harrington, W. F.; Berger, A.; Sela, M.; Katchalski, E. J. Am. Chem. Soc. 1960, 82, 5263–5274. Horng, J. C; Raines, R. T. Protein Science 2006, 15, 74–86.
- Gornick, F.; Mandelkern, L., Diorio, A. F.; Roberts, D. E. J. Am. Chem. Soc. 1964, 86, 2549–2562.
- Mattice, W. L.; Mandelkern, L. Biochemistry 1970, 9, 1049–1063.
- Mattice, W. L; Mandelkern, L. J. Am. Chem. Soc. 1971, 93, 1769–1782.
- Johnston, N.; Krimm, S. Biochemistry 1971, 10, 2597–2611.
- Schleich, T.; Yeh, Y. Biopolymers 1973, 12, 993–1010.
- Lerner, D. A.; Schleich, T. Biopolymers 1973, 12, 1011–1021.
- Clark, D. S.; Dechter, J. J.; Mandelkern, L. Macromolecules 1973, 12, 626–640.
- Darsey, J. A.; Mattice, W. L. Macromolecules 1982, 15, 1626–1639.
- Zhuang, X; Rief, M. Curr. Opin. Struct. Biol. 2003, 13, 88–97.
- Kim HD. Bull. Korean Chem. Soc. 1997, 18, 922–927.
- Zagrovic, B.; Lipfert, J.; Sorin, E. J.; Millet, I. S.; van Gunsteren, W. F.; Doniach, S. V.; Pande, V. S. Proc. Natl. Acad. Sci. 2005, 102, 11698–11724.
- Zhong, H.; Carlson, H. A. J. Chem. Theory Comput. 2006, 2, 342–354.
- Stapley, B. J.; Creamer, T. P. Protein Science 1999, 8, 587–601.
- Rucker, A. L.; Creamer, T. P. Protein Science 2002, 11, 980–994.
- Andries, J. C.; Walton, A. G. Biopolymers 1969, 8, 523–529.
- Brown, B. L.; Jennings, B. R. Biopolymers 1970, 9, 1119–1132.
- Marchal, E.; Dufour, C. Biopolymers 1972, 11, 1021–1035.
- Kubota, K.; Tominaga, Y.; Fujime, S. Macromolecules 1986, 19, 1604–1618.
- Teraoka, I. Polymer Solutions: an Introduction to Physical Properties. John Wiley & Sons Inc., NY, USA, 2002, 187–200.
- Wissenburg, P.; Odijk, T.; Kuil, M.; Mandel, M. Polymer Comm. 1992, 33, 5328–5340.
- Wissenburg, P.; Odijk, T.; Cirkel, P.; Mandel, M. Macromolecules 1995, 28, 2315–2327.
- Cohen, J; Priel, Z. J. Chem. Phys. 1990, 93, 9062–9068.
- Antonietti, M.; Briel, A.; F¨ oster, S. J. Chem. Phys. 1996, 105, 7795–7806.
- Eisenberg, H. Acta Polym. 1998, 49, 534–547.
- Wolf, B. A. Macromol. Rapid Commun. 2007, 28, 164–172.