Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery

The aim of this study was to prepare and evaluate stable cationic solid lipid nanoparticles (SLNs) as colloidal carriers for gene therapy. SLNs were mainly composed of three different biocompatible and biodegradable matrix lipids called tripalmitin, glyceryl dibehenate, and triglyceride, all containing the cationic lipid $N$-(1-(2,3-dioleoyloxy)propyl)-$N,N,N$-trimethylammonium (DOTAP$^®$). Each of these SLNs were divided into three parts and characterized by applying different processes: Part I was autoclaved (121 $^{\circ}$C, 15 min), Part II was lyophilized (5 mbar, --50 $^{\circ}$C), and Part III was kept in its intact form. These parts were stored at 4 $^{\circ}$C, 25 $^{\circ}$C (room temp.), and 40 $^{\circ}$C for short-term stability tests. The formulations were tested physically regarding the particle size and zeta potential. pUC18 plasmid DNA was used as the genetic material. Zeta potentials of all SLNs and pDNA-SLN complexes were determined to be highly positive (between +28.90 and +59.39). Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP$^®$. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.

Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery

The aim of this study was to prepare and evaluate stable cationic solid lipid nanoparticles (SLNs) as colloidal carriers for gene therapy. SLNs were mainly composed of three different biocompatible and biodegradable matrix lipids called tripalmitin, glyceryl dibehenate, and triglyceride, all containing the cationic lipid $N$-(1-(2,3-dioleoyloxy)propyl)-$N,N,N$-trimethylammonium (DOTAP$^®$). Each of these SLNs were divided into three parts and characterized by applying different processes: Part I was autoclaved (121 $^{\circ}$C, 15 min), Part II was lyophilized (5 mbar, --50 $^{\circ}$C), and Part III was kept in its intact form. These parts were stored at 4 $^{\circ}$C, 25 $^{\circ}$C (room temp.), and 40 $^{\circ}$C for short-term stability tests. The formulations were tested physically regarding the particle size and zeta potential. pUC18 plasmid DNA was used as the genetic material. Zeta potentials of all SLNs and pDNA-SLN complexes were determined to be highly positive (between +28.90 and +59.39). Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP$^®$. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.

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Bibtex @ { tbtkchem143436, journal = {Turkish Journal of Chemistry}, issn = {1300-0527}, eissn = {1303-6130}, address = {}, publisher = {TÜBİTAK}, year = {2015}, volume = {39}, pages = {1012 - 1024}, doi = {}, title = {Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP\$\^®\$ for DNA delivery}, key = {cite}, author = {Büyükköroğlu, GÜLAY and Yazan, EMİNE YASEMİN and Öner, AYŞE FİLİZ} }
APA Büyükköroğlu, G , Yazan, E , Öner, A . (2015). Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery . Turkish Journal of Chemistry , 39 (5) , 1012-1024 .
MLA Büyükköroğlu, G , Yazan, E , Öner, A . "Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery" . Turkish Journal of Chemistry 39 (2015 ): 1012-1024 <
Chicago Büyükköroğlu, G , Yazan, E , Öner, A . "Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery". Turkish Journal of Chemistry 39 (2015 ): 1012-1024
RIS TY - JOUR T1 - Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery AU - GÜLAY Büyükköroğlu , EMİNE YASEMİN Yazan , AYŞE FİLİZ Öner Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Chemistry JF - Journal JO - JOR SP - 1012 EP - 1024 VL - 39 IS - 5 SN - 1300-0527-1303-6130 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Chemistry Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery %A GÜLAY Büyükköroğlu , EMİNE YASEMİN Yazan , AYŞE FİLİZ Öner %T Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery %D 2015 %J Turkish Journal of Chemistry %P 1300-0527-1303-6130 %V 39 %N 5 %R %U
ISNAD Büyükköroğlu, GÜLAY , Yazan, EMİNE YASEMİN , Öner, AYŞE FİLİZ . "Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery". Turkish Journal of Chemistry 39 / 5 (Aralık 2015): 1012-1024 .
AMA Büyükköroğlu G , Yazan E , Öner A . Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery. Turk J Chem. 2015; 39(5): 1012-1024.
Vancouver Büyükköroğlu G , Yazan E , Öner A . Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery. Turkish Journal of Chemistry. 2015; 39(5): 1012-1024.
IEEE G. Büyükköroğlu , E. Yazan ve A. Öner , "Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP$^®$ for DNA delivery", Turkish Journal of Chemistry, c. 39, sayı. 5, ss. 1012-1024, Ara. 2015
Turkish Journal of Chemistry
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

37.5b15.9b

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