A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025--1.0 mg/mL and 1.0-100 $\mu $g/mL concentrations for 24 h, the IC$_{50}$ values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.

A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025--1.0 mg/mL and 1.0-100 $\mu $g/mL concentrations for 24 h, the IC$_{50}$ values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.

___

  • min at 37 ◦
  • C, protected from light. Cells were washed with PBS and DAPI fluoroshield was dropped on them. Finally images were taken using a fluorescence microscope, Olympus IX50, equipped with an Olympus SC30 camera.
  • Cell proliferation
  • Statistical analysis All experiments were repeated 4 times. Statistical analysis was carried out with the GraphPad Prism 6 statistical software. One-way analysis of variance (ANOVA) was performed with Tukey’s test for multiple comparisons in statistical evaluation. The difference between two groups was considered to be significant when the P value was less than 0.05.
Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Enhanced electrocatalytic efficiency of C/MWNTs for methanol oxidation using Ni deposited on MWNTs

Murat FARSAK, Gülfeza KARDAŞ

Synthesis and biological evaluation of a new series of 4-alkoxy-2-arylquinoline derivatives as potential antituberculosis agents

Gonca TOSUN, Tayfun ARSLAN, Zeynep İSKEFİYELİ, Murat KÜÇÜK, Şengül Alpay KARAOĞLU, Nurettin YAYLI

Palladium nanoparticles supported on modified polystyrene resin as a polymeric catalyst for Sonogashira-Hagihara coupling reactions

BAHMAN TAMAMI, FATEMEH NOWROOZI DODEJI, SOHEILA GHASEMI

Optimization of the adsorption of a textile dye onto nanoclay using a central composite design

Aydin HASSANI, Murat KIRANŞAN, Reza Darvishi Cheshmeh SOLTANI, Alireza KHATAEE, Semra KARACA

Three-component synthesis of cyclic β -aminoesters using CeO2 nanoparticles as an efficient and reusable catalyst

Javad GHOMI SAFAEI, Sima KALHOR, Hossein ALAVI SHAHBAZI, Mehrnoosh KHEIRABADI ASGARI

Purification, refolding, and characterization of recombinant human paraoxonase-1

Yeliz DEMİR, Şükrü BEYDEMİR

Conversion of racemic allylic hydroperoxides into corresponding chiral 1/2,3-triols by using catalytic OsO$_{4}$ and chiral cinchona ligands in the absence of co-oxidant

Haydar GÖKSU, Mehmet Serdar GÜLTEKİN

Synthesis and characterization of novel urea and thiourea substitute cyclotriphosphazene compounds as naked-eye sensors for F − and CN− anions

Özgür ÖZAY, Mehmet YILDIRIM, Hava OZAY

Microwave-assisted synthesis of novel 8-chloro-[1,2,4]triazolo[4,3-$a$]pyridine derivatives

LINJIONG ZHANG, MINGYAN YANG, Beizhen HU, ZHAOHUI SUN, XINGHAI LIU, JIANQUAN WENG, CHENGXIA TAN

A comparative study on fabrication of Cu$_{2}$ZnSnS$_{4}$ (CZTS) nanofibers using acetate and chloride metal precursors

BURAK ZAFER BÜYÜKBEKAR, FARUK ÖZEL, HÜSEYİN ŞAKALAK, HALİT ÇAVUŞOĞLU, MUSTAFA ERSÖZ, MAHMUT KUŞ, MUSTAFA SELMAN YAVUZ