The role of BADH gene in oxidative, salt, and drought stress tolerances of white clover

The aim of this study was to confirm the presence of BADH gene and control whether it plays an important role to protectwhite clover (Trifolium repens L.) against oxidative, salt, and drought stresses. Three genotypes possessing BADH and 1 genotype lackingBADH were selected, considering the expression levels of BADH in response to methyl viologen mediated oxidative stress. The genotypespossessing BADH displayed significantly less ion leakage under oxidative stress treatment, compared to that lacking BADH. In orderto test salinity tolerance, the plants were treated with 300 mM NaCl for 2 weeks, and to mimic drought conditions, plants were grownwithout irrigating for 5 days. Under the salt stress, the plants possessing BADH contained lower malondialdehyde levels and higherchlorophyll contents than that of plants lacking BADH. Similarly, under the drought conditions, the plants possessing BADH were foundto have increased levels of glycinebetaine, proline, and high relative water contents than the plant lacking BADH. These findings indicatethat the plants having BADH exhibited enhanced tolerance to salt and drought stresses. These enhanced tolerances of plants possessingBADH gene is attributed to its ability to induce glycinebetaine synthesis. Moreover, the plants possessing BADH were found to exhibithigher relative feed value under both stress and normal conditions. These plants could be used as forage crops on marginal areas havingsalinity or drought problems.

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