An integrated approach to the study of Hypericum occurring in Sicily

An integrated approach to the study of Hypericum occurring in Sicily

An integrated approach to the study of taxa of the genus Hypericum occurring in Sicily is proposed. The results ofmorphological, biochemical, and molecular analyses are combined to better assess the relationships between the species investigated andtest the suitability of DNA barcoding technique in the discrimination of these taxa. For the name Hypericum aegypticum subsp. webbii(Spach) N. Robson a lectotype is designated. For Hypericum triquetrifolium Turra a lectotype and a supporting epitype are designated.The presence of Hypericum perforatum L. subsp. perforatum is excluded from Sicily and the previous reports have to be referred to H.perforatum subsp. veronense (Schrank) Ces. Hypericum perfoliatum L. and H. pubescens Boiss. are close morphologically and chemically,as well as based on the results from rcbL marker, although belonging to different sections. Biochemical analyses confirmed the relevantamounts in bioactive metabolites of the studied taxa. Hypericum perfoliatum L. is proposed as a valid alternative to H. perforatum L. forcultivation with phytotherapic purposes.

___

  • Bartolucci F, Peruzzi L, Galasso G, Albano A, Alessandrini A et al. (2018). An updated checklist of the vascular flora native to Italy. Plant Biosystems 152: 179-303. doi: 10.1080/11263504.2017.1419996
  • Bazan G, Marino P, Guarino R, Domina G, Schicchi R (2015). Bioclimatology and vegetation series in Sicily: A geostatistical approach. Annales Botanici Fennici 52: 1-18.
  • Boccone P (1697). Museo di piante rare della Sicilia, Malta, Corsica, Italia, Piemonte, e Germania. Venetia: Baptistam Zuccato (in Italian).
  • Bombardelli E, Morazzoni P (1995). Hypericum perforatum. Fitoterapia 66: 43-68.
  • Burdet HML, Charpin A, Jacquemoud F (1984). Types nomenclaturaux de taxa iberiques decrits par Boissier ou Reuter. VI. Euphorbiacees a Guttiferes. Candollea 39: 771-789 (in French).
  • Burtt BL, Davis PH (1949). On the flora of the Nearer East: XXIII. Miscellaneous new species and records. Kew Bulletin 4: 97- 115.
  • Castellano G, Spadaro V (2010). Hypericum calycinum (Clusiaceae) in Sicilia: aspetti farmacognostici e corologici. Quaderni di Botanica Ambientale e Applicata 21 (2010): 29-32 (in Italian).
  • Chase MW, Salamin N, Wilkinson M, Dunwell JM, Kesanakurthi RP et al. (2005). Land plants and DNA barcodes: short-term and long-term goals. Philosophical Transactions of the Royal Society B: Biological Sciences 360: 1889-1895. doi: 10.1098/ rstb.2005.1720
  • Ciccarelli D, Garbari F (2004). Le unità italiane di Hypericum (Clusiaceae), serie Hypericum. Informatore Botanico Italiano 36: 413-424 (in Italian).
  • Crockett SL, Robson NKB (2011). Taxonomy and chemotaxonomy of the genus Hypericum. Medicinal and Aromatic Plant Science and Biotechnology 5 (1): 1-13.
  • Domina G (2018). Host-driven morphological variability in Orobanche crenata (Orobanchaceae). Turkish Journal of Botany 42: 502-509 doi:10.3906/bot-1712-25.
  • Domina G, Scibetta S, Scafidi F, Giovino A (2017). Contribution to the identification of Dianthus rupicola (Caryophyllaceae) subspecies using morphological and molecular approaches. Phytotaxa 291: 17-32. doi:10.11646/phytotaxa.291.1.2.
  • Domina G, Venturella G, Gargano ML (2018). Synthetic cartography for mapping biodiversity in the Mediterranean region: Sicily as case study. PhytoKeys 109: 77-92. doi:10.3897/ phytokeys.109.28297
  • Doyle JJ, Doyle JL (1987). A rapid isolation procedure for small amounts of leaf tissue. Phytochemical Bulletin 19: 11-15.
  • Dunning LT, Savolainen V (2010). Broad-scale amplification of matK for DNA barcoding plants, a technical note. Botanical Journal of the Linnean Society 164: 1-9. doi: 10.1111/j.1095- 8339.2010.01071.x
  • Fascella G, Airò M, Mammano MM, Giardina G, Carrubba A et al. (2017). Rooting and acclimatization of micropropagated Hypericum perforatum L. native to Sicily. Acta Horticulturae 1155: 543-548. doi: 10.17660/ActaHortic.2017.1155.80
  • Fazekas AJ, Kuzmina ML, Newmaster SG, Hollingsworth PM (2012). DNA Barcoding Methods for Land Plants. Methods in Molecular Biology 858: 223-252. doi:10.1007/978-1-61779-591-6_11
  • Galasso G, Conti F, Peruzzi L, Ardenghi NMG, Banfi E et al. (2018). An updated checklist of the vascular flora alien to Italy. Plant Biosystems 152: 556-592. doi: 10.1080/11263504.2018.1441197
  • Giovino A, Domina G, Bazan G, Campisi P, Scibetta S (2015). Taxonomy and conservation of Pancratium maritimum (Amaryllidaceae) and relatives in the Central Mediterranean. Acta Botanica Gallica: Botany Letters 162: 289-299. doi:10.1080 /12538078.2015.1089416
  • Giovino A, Marino P, Domina G, Scialabba A, Schicchi R et al. (2016). Evaluation of the DNA barcoding approach to develop a reference data-set for the threatened flora of Sicily. Plant Biosystems 150: 631-640. doi: 10.1080/11263504.2014.989285
  • Hammer Ø (2019). PAST 3.26b.
  • Hammer Ø, Harper DAT, Ryan PD (2001). PAST: paleontological Statistics software package for education and data analysis. Palaeontologia Electronica 4: 1-9.
  • Hollingsworth PM, CBOL Plant Working Group (2009). A DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America 106: 12794-12797. doi: 10.1073/pnas.0905845106
  • Hosni K, Msaada K, Ben Taârit M, Marzouk B (2011). Phenological variations of secondary metabolites from Hypericum triquetrifolium Turra. Biochemical Systematics and Ecology 39: 43-50. doi: 10.1016/j.bse.2011.01.001
  • İpek M, İpek A, Simon PW (2014). Testing the utility of matK and ITS DNA regions for discrimination of Allium species. Turkish Journal of Botany 38: 203-212. doi: 10.3906/bot-1308-46
  • Kimura M (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16 (2): 111-120.
  • Lazzara S, Napoli E, Carrubba A (2015). Hypericum spp.: A Resource from Wild Mediterranean Flora for the Treatment of Mild Depression. In: V.K. Gupta (editor). Bioactive Phytochemicals: Perspectives for Modern Medicine. New Delhi, India: Daya Publishing House, pp. 337-353.
  • Lazzara S, Carrubba A, Napoli E (2020). Variability of hypericins and hyperforin in Hypericum species from the Sicilian flora. Chemistry & Biodiversity 17: e1900596. doi: 10.1002/ cbdv.201900596
  • Napoli E, Siracusa L, Ruberto G, Carrubba A, Lazzara S et al. (2018). Phytochemical profiles, phototoxic and antioxidant properties of eleven Hypericum species. A comparative study. Phytochemistry 152: 162-173. doi: http://dx.doi.org/10.1016/j. phytochem.2018.05.003
  • Peruzzi L, Galasso G, Domina G, Bartolucci F, Santangelo A et al. (2019). An inventory of the names of native, non-endemic vascular plants described from Italy, their loci classici and types. Phytotaxa 410 (1): 1-215. doi: 10.11646/phytotaxa.410.1.1
  • Ratnasingham S, Hebert PDN (2007). BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes 7 (3): 355-364. doi: 10.1111/j.1471-8286.2007.01678.x
  • Robson NKB, Adams P (1968). Chromosome numbers in Hypericum and related genera. Brittonia 20 (2): 95.
  • Robson NKB (1985). Studies in the genus Hypericum L. (Guttiferae) 3. Sections 1. Campylosporus to 6a. Umbraculoides. Bulletin of the British Museum of Natural History (Botany) 12: 163-325.
  • Robson NKB (1993). Studies in Hypericum: validation of new names. Bulletin of the Natural History Museum London (Botany Series) 23 (2): 67-70.
  • Robson NKB (2002). Studies in the genus Hypericum L. (Guttiferae) 4 (2). Section 9. Hypericum sensu lato (part 2): subsection 1. Hypericum series 1. Hypericum. Bulletin of the Natural History Museum London (Botany Series) 32 (2): 61-123. doi: 10.1017/ S096804460200004X
  • Robson NKB (2010). Studies in the genus Hypericum L. (Guttiferae) 6. Sections 20. Myriandra to 28. Elodes. Bulletin of the Natural History Museum London (Botany Series) 26 (2): 75-217.
  • Robson NKB, Carine M, Pattison D, Nürk N, Crockett S et al. (2019). Hypericum online.
  • Saitou N, Nei M (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
  • Stafleu FA, Cowan RS (1986). Taxonomic literature, 2nd ed. Utrecht, The Hague, Boston: Scheltema & Holkema, Bohn, Junk Publ., pp. 551-552.
  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729. doi: 10.1093/molbev/mst197
Turkish Journal of Botany-Cover
  • ISSN: 1300-008X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

An attempt to solve the taxonomic confusion of Thlaspiceras F.K.Mey. complex (Noccaea Moench-Brassicaceae) with ISSRs

Burcu TARIKAHYA HACIOĞLU, Kurtuluş ÖZGİŞİ, Atila OCAK

An integrated approach to the study of Hypericum occurring in Sicily

Alessandra CARRUBBA, Antonio GIOVINO, Silvia LAZZARA, Edoardo NAPOLI, Gianniantonio DOMINA

Kurtuluş ÖZGİŞİ, Atila OCAK, Burcu Tarıkahya HACIOĞLU

Esra MARTİN, Ahmet KAHRAMAN, Tuncay DİRMENCİ, Havva BOZKURT, Halil Erhan EROĞLU

Karyotypic phylogeny and polyploidy variations of Paronychia (Caryophyllaceae) taxa in Turkey

Derya ALTAY, Halil Erhan EROĞLU, Ümit BUDAK, Esra MARTİN

Effects of environmental heterogeneity and temporal variation on ecological relationships of vegetation in a seasonally dry tropical forest in Brazil

Felipe de Carvalho ARAÚJO, Natália de AGUIAR-CAMPOS, Vinicius Andrade MAIA, Diego Teixeira GIRARDELLI, Rubens Manoel dos SANTOS, Gabriela Gomes PIRES, Nathalle Cristine Alencar FAGUNDES, Wilder Bento da SILVA, Alisson Borges Miranda SANTOS, Polyanne Aparecida COELHO, Jean Daniel MOREL, Cléber Rodrig

The role of BADH gene in oxidative, salt, and drought stress tolerances of white clover

Gürkan DEMİRKOL

Ahmet AKSOY, Taner ÖZCAN, Hüseyin GİRİŞKEN, Jale ÇELİK, Tuncay DİRMENCİ

Karyotype evolution and new chromosomal data in Erodium: chromosome alteration, polyploidy, dysploidy, and symmetrical karyotypes

Tuncay DİRMENCİ, Halil Erhan EROĞLU, AHMET KAHRAMAN, Havva BOZKURT, Esra MARTİN

Angana BORAH, Deepak KUMAR, Manabı PAW, Twahıra BEGUM, Mohan LAL