Iwona JEDRZEJCZYK, Maria MOROZOWSKA, Renata NOWIŃSKA, Andrzej M. JAGODZIŃSKI

Primula veris plants derived from in vitro cultures and from seeds: genetic stability, morphology, and seed characteristics

Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during themicropropagation process. The mean 2C DNA content was 0.97 pg/2C and P. veris can be classified in the group of plants of verysmall genomes. Comparative morphological analysis of cowslip plants regenerated in vitro and derived from seeds was carried out inthree subsequent years under common garden conditions, which limited the effects of environmental factors. The origin of specimenssignificantly influenced most of the morphological traits examined. Plants that originated from seeds were characterized by moreintensive development rate and higher total seed production compared to micropropagated plants. Significant differences betweenthe two groups of specimens were observed for the number of flower stalks, flowers, fruits, and seeds per plant. No interseasonalvariation within the reproductive traits such as number of seeds per flower stalk and per plant was found for plants derived from invitro cultures. That allows prediction of seed production from in vitro regenerated plants after their successful acclimatization to fieldconditions. Importantly, plants derived from in vitro cultures set significantly bigger seeds. Our results are promising for broad uses ofthe regenerated in vitro cowslip plants in ornamental horticulture and the pharmaceutical industry.

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