Habitat effects and differences in the reproductive success of Orchis punctulata and Orchis purpurea (Orchidaceae)

The pollination success of two nonrewarding orchids in two coarse habitats was examined. The comparative results of Orchis punctulata and O. purpurea showed a significant differentiation. The fruit set of O. purpurea was significantly greater compared to O. punctulata, whereas both species also had significantly higher fruit set in the grassland compared to the forest. Although the pollination success of O. purpurea was not significantly correlated with plant height, inflorescence size, or nearest neighbor distance, these factors were found to be significantly correlated to the pollination success of O. punctulata. Among these factors, the nearest neighbor distance had the highest impact on the pollination success of O. punctulata, whereas the respective effects to the individuals of O. purpurea were found to be nonsignificant. The results demonstrate that the distribution of O. punctulata at the edges of its range is not delimited by factors related to its pollination effectiveness and that other factors, mostly related to the microsite conditions, might play a more significant role in the colonization of more western areas. Moreover, suitable management treatments that would increase the light levels within the forest sites studied may also increase the pollination success and the fruit production of the studied orchids.

Keywords:

Orchids, deception, inflorescence size, nearest neighbor distance female reproductive success, conservation,

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Baumann H, Künkele S, Lorenz R (2006). Die Orchideen Europas mit angrenzenden Gebieten. Stuttgart, Germany: Eugen Ulmer KG.
Cameron DD, Johnson I, Read DJ, Leake JR (2008). Giving and receiving: measuring the carbon cost of mycorrhizas in the green orchid Goodyera repens . New Phytol 180: 176-184.
Carrió E, Jiménez JF, Sánchez-Gómez P, Guemes J (2009). Reproductive biology and conservation implications of three endangered snapdragon species ( Antirrhinum , Plantaginaceae). Biol Conserv 142: 1854-1863.
Claessens J, Kleynen J (2011). The Flower of the European Orchid. Form and Function. Schrijen-Lippertz, the Netherlands: Jean Claessens and Jacques Kleynen.
Cozzolino S, Aceto S, Caputo P, Widmer A, Dafni A (2001). Speciation processes in Eastern Mediterranean Orchis s.l. species: molecular evidence and the role of pollination biology. Israel J Plant Sci 49: 91-103.
Dafis S (1973). Classification of the forest vegetation of Greece. Sci Ann Dep Forest Nat Env 115: 75-91 (in Greek).
Dressler RL (1981). The Orchids: Natural History and Classification. Cambridge, MA, USA: Harvard University Press.
Ferdy JB, Austerlitz F, Moret J, Gouyon PH, Godelle B (1999). Pollinator-induced density dependence in deceptive species. Oikos 87: 549-560.
Fritz AL (1990). Deceit pollination of Orchis spitzelii (Orchidaceae) on the island of Gotland in the Baltic: a suboptimal system. Nord J Bot 9: 577-587.
Govaerts R (2016). World Checklist of Orchidaceae. Facilitated by the Royal Botanic Gardens, Kew. Website http://apps.kew.org/ wcsp/ [accessed 4 November 2016].
Gumbert A, Kunze J (2001). Colour similarity to rewarding model plants affects pollination in a food deceptive orchid, Orchis boryi . Biol J Linn Soc 72: 419-433.
Hansen I, Olesen JM (1999). Comparison of reproductive success in two orchids: the nectarless Dactylorhiza majalis s.s. and the nectar-producing Gymnadenia conopsea s.l. Nord J Bot 19: 665-671.
Hobbhahn N, Johnson SD, Harder LD (2017). The mating consequences of rewarding vs. deceptive pollination systems: is there a quantity-quality trade-off ? Ecol Monogr 87: 91-104.
Jacquemyn H, Brys R (2010). Temporal and spatial variation in flower and fruit production in a food-deceptive orchid: a five- year study. Plant Biol 12: 145-153.
Jacquemyn H, Brys R, Hermy M (2002). Flower and fruit production in small populations of Orchis purpurea and implications for management. In: Kindlmann P, Willems JH, Whigham DF, editors. Trends and Fluctuations and Underlying Mechanisms in Terrestrial Orchid Populations. Leiden, the Netherlands: Backhuys Publishers, pp. 67-84.
Jacquemyn H, Brys R, Hermy M, Willems JH (2005). Does nectar reward affect rarity and extinction probabilities of orchid species? An assessment using historical records from Belgium and the Netherlands. Biol Conserv 121: 257-263.
Jacquemyn H, Brys R, Honnay O, Hutchings MJ (2009). Biological flora of the British Isles: Orchis mascula (L.) L. J Ecol 97: 360- 377.
Jacquemyn H, Brys R, Vandepitte K, Honnay O, Roldán-Ruiz I, Wiegand T (2007). A spatially-explicit analysis of seedling recruitment in the terrestrial orchid Orchis purpurea . New Phytol 176: 448-459.
Jacquemyn H, Duffy KJ, Sellose MA (2017). Biogeography of orchid mycorrhizas. In: Tedersoo L, editor. Biogeography of Mycorrhizal Symbiosis, Ecological Studies 230. Springer International Publishing AG, pp. 159-177.
Jermakowicz E, Ostrowiecka B, Tałałaj I, Pliszko A, Kostro- Ambroziak A (2015). Male and female reproductive success in natural and anthropogenic populations of Malaxis monophyllos (L.) Sw. (Orchidaceae). Biodiv Res Conserv 39: 37-44.
Jersáková J, Johnson SD, Kindlmann P (2006). Mechanisms and evolution of deceptive pollination in orchids. Biol Rev 81: 219- 235.
Jersáková J, Jürgens A, Šmilauer P, Johnson SD (2012). The evolution of floral mimicry: identifying traits that visually attract pollinators. Funct Ecol 26: 1381-1389.
Jersáková J, Spaethe J, Streinzer JN, Paulus H, Dötterl S, Johnson SD (2016). Does Traunsteinera globosa (the globe orchid) dupe its pollinators through generalized food deception or mimicry? Bot J Linn Soc 180: 269-294.
Johnson SD, Bond WJ (1997). Evidence for widespread pollen limitation of fruiting success in Cape wildflowers. Oecologia 109: 530-534.
Johnson SD, Peter CI, Nilsson LA, Ågren J (2003). Pollination success in a deceptive orchid is enhanced by co-occurring rewarding magnet plants. Ecology 84: 2919-2927.
Juillet N, Gonzalez MA, Page PA, Gigord LDB (2007). Pollination of the European food-deceptive Traunsteinera globosa (Orchidaceae): the importance of nectar-producing neighboring plants. Plant Syst Evol 265: 123-129.
Kindlmann P, Jersáková J (2006). Effects of floral display on reproductive success in terrestrial orchids. Folia Geobot 41: 47-60.
Kretzschmar H, Eccarius W, Dietrich H (2007). The Orchid Genera Anacamptis , Orchis and Neotinea . Phylogeny, Taxonomy, Morphology, Biology, Distribution, Ecology and Hybridisation. Bürgel, Germany: EchinoMedia Verlag.
Kreutz CAJ (2004). The Orchids of Cyprus. Landgraaf, the Netherlands: C.A.J. Kreutz. Lang D (2004). Britain’s Orchids. Old Basing, UK: Wild Guides.
Levin DA, Anderson WW (1970). Competition for pollinators between simultaneously flowering species. Amer Nat 104: 455- 467.
Machaka-Houri N, Al-Zein M.S, Westbury DB, Talhouk SN (2012). Reproductive success of the rare endemic Orchis galilaea (Orchidaceae) in Lebanon. Turk J Bot 36: 677-682.
Münzbergová Z (2005). Determinants of species rarity: population growth rates of species sharing the same habitat. Am J Bot 92: 1987-1994.
Neiland MRM, Wilcock CC (1998). Fruit set, nectar reward, and rarity in the Orchidaceae. Am J Bot 85: 1657-1671.
Nilsson LA (1992). Orchid pollination biology. Trends Ecol Evol 7: 255-259.
Petanidou T, Lamborn E (2005). A land for flowers and bees: studying pollination ecology in Mediterranean communities. Plant Biosyst 139: 279-294.
Peter CI, Johnson SD (2008). Mimics and magnets: the importance of color and ecological facilitation in floral deception. Ecology 89: 1583-1595.
Pfeifer M, Passalacqua NG, Bartram S, Schatz B, Croce A, Carey PD, Kraudelt H, Jeltsch F (2010). Conservation priorities differ at opposing species borders of a European orchid. Biological Conservation 143: 2207-2220.
R Core Team (2013). R: A language and environment for statistical computing. Website http://www.R-project.org [accessed 03 February 2016].
Rasmussen H (1995). Terrestrial orchids from seed to mycotrophic plant. Cambridge, UK: Cambridge University Press.
Roberts DL (2003). Pollination biology: the role of sexual reproduction in orchid conservation. In: Dixon KW, Kell SP, Barrett RL, Cribb PJ, editors. Orchid Conservation. Kota Kinabalu, Sabah, Borneo: Natural History Publications, pp. 113-136.
Rymer PD, Whelan RJ, Ayre DJ, Weston PH, Russell KG (2005). Reproductive success and pollinator effectiveness differ in common and rare Persoonia species (Proteaceae). Biol Conserv 123: 521-532.
Schatz B, Delle-Vedone R, Dormont L (2013). Presence, distribution and effect of white, pink and purple morphs on pollination in the orchid Orchis mascula . Eur J Env Sci 3: 119-128.
Sletvold N, Trunschke J, Smit M, Verbeek J, Ågren J (2016). Strong pollinator-mediated selection for increased flower brightness and contrast in a deceptive orchid. Evolution 70: 716-724.
Steffan-Dewenter I, Westphal C (2008). The interplay of pollinator diversity, pollination services and landscape change. J Appl Ecol 45: 737-741.
Tremblay RL, Ackerman JD, Zimmerman JK, Calvo RN (2005). Variation in sexual reproduction in orchids and its evolutionary consequences: a spasmodic journey to diversification. Biol J Linn Soc 84: 1-54.
Tsiftsis S, Antonopoulos Z (2017). Atlas of the Greek Orchids, Vol I. Rethymno, Greece: Mediterraneo Editions.
Tsiftsis S, Tsiripidis I (2016). Threat categories of the Greek orchids (Orchidaceae). Bot Chron 21: 43-74.
Vakhrameeva MG, Tatarenko IV, Varlygina TI, Torosyan GK, Zagulskii MN (2008). Orchids of Russia and adjacent countries (within the borders of the Former USSR). Ruggell/ Liechtenstein: A.R.G. Gantner Verlag.
Van der Cingel NA (1995). An Atlas of Orchid Pollination. European Orchids. Rotterdam, the Netherlands: A.A. Balkema.