Phylogenetic relationships of some Turkish Crocus (Iridaceae) taxa based on morphological and anatomical characters

In this study, relationships among the 15 taxa of the genus Crocus L. distributed in Turkey were analysed using 29 morphological and 4 anatomical characters. Analysis of the data set utilising maximum parsimony criterion with Branch-and-Bound search algorithm yielded 32 most parsimonious trees. Bootstrap analysis with the majority rule consensus algorithm generated a consensus tree supporting some branches. Our data mostly did not agree with the previous sectional and serial treatments. The most interesting result was the condition of Crocus pallasii, which was previously included in the section Crocus. C. pallasii showed a sister group relationship with C. cancellatus from the section Nudiscapus series Reticulati in this work. Previously described sections Flavi and Reticulati did not show monophyly for their taxa based on our analysis (e.g., C. gargaricus and C. cancellatus of the section Reticulati were not monophyletic based on our data set). Interestingly, C. gargaricus of the section Reticulati was sister to the section Nudiscapus series Biflori members, specifically with C. leichtlinii. Sectional and serial treatment of C. fleischeri was consistent with our results. Our data suggest that more morphological data along with molecular data are needed for reliable conclusions on the studied taxa.

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Key words: Anatomy, Crocus, morphology, parsimony, phylogenetic

Phylogenetic relationships of some Turkish Crocus (Iridaceae) taxa based on morphological and anatomical characters

Keywords:

Key words: Anatomy, Crocus, morphology, parsimony, phylogenetic,

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Akan H & Eker I (2004). Some Morphological and Anatomical Investigations on Autumn Species of Crocus L. Occurring in Şanlıurfa. Turk J Bot 28: 185-191.
Akan H, Satıl F & Eker I (2007). The Morphological And Anatomical Properties of Endemic Crocus leichtlinii (D.Dewar) Bowles (Iridaceae) in Turkey. Pak J Bot 36: 711-718.
Alavi-Kia SS, Mohammadi SA, Aharizad S & Moghaddam M (2008). Analysis of Genetic Diversity and Phylogenetic Relationships in Crocus Genus of Iran Using Inter-Retrotransposon Amplified Polymorphism. Biotechnol & Biotechnol Eq 22: 795-800.
Ali SI & Mathew B (2000). Iridaceae. In: Ali SI & Qaiser M (eds.), Flora of Pakistan, 202: 1-35, St. Louis: Press of University of Karachi and Missouri Botanical Garden.
Almeida VR, Costa AF, Mantovani A, Mantovani A, Gonçalves-Esteves V, Aruuda RCO & Forzza RC (2009). Morphological Phylogenetics of Quesnelia (Bromeliaceae, Bromelioideae) Sys Bot 34: 660-672.
Baytop A (1972). Anatomical structure of plant drugs. Pharmacy Faculty, Publication 829, Istanbul.
Candan F, Kesercioğlu T & Şık L (2009). Micromorphological Investigations on Pollen Samples of Four Yellow Flowered Taxa of Crocus L. (Iridaceae) from Turkey. JABS 3(2): 56-59.
Erol O & Küçüker, O (2005). The crocus of İstanbul. The Plantsman 4(3): 168-169.
Erol O & Küçüker, O (2007). Leaf Anatomy of Some Endemic Crocus L. (Iridaceae) Taxa from the West Anatolia. Int J Bot 3 (3): 290-295.
Felsenstein J (1985). Confidence limits on phylogenies: An approach using bootstrap. Evolution 39: 783-791.
Frello S & Harrison JSH (2000). Chromosomal Variation in Crocus vernus Hill (Iridaceae) Investigated by in situ Hybridization of rDNA and a Tandemly Repeated Sequence. Ann Bot 86: 317-322.
Işık S & Dönmez EO (2006). Pollen Morphology of Some Turkish Crocus L. (Iridaceae) Species. Acta Biol Cracov Bot 48: 85-91.
Kerndorff H & Pasche E (1994). Crocus mathewii. A new Autumn- Flowering Crocus from Turkey. New Plantsman 1: 102-106.
Kerndorff H & Pasche E (1996a). Crocuses from Turkey to Jordan. Quart Bull Alp Gard Soc 64: 296-312.
Kerndorff H & Pasche E (1996b). Crocuses from Turkey to Jordan (Part 2). Quart Bull Alp Gard Soc 64: 459-467.
Kerndorff H & Pasche E (1997). Two remarkable taxa of the Crocus biflorus complex (Iridaceae) from northeastern Turkey. Linzer Biol Beitr 29: 591-600.
Kerndorff H & Pasche E (2004). Two New Taxa of the Crocus biflorus Aggregate (Liliiflorae, Iridaceae) from Turkey Linzer Biol Beitr 36(1): 5-10.
Martin E, Dinç M & Duran A (2009). Karyomorphological Study of Eight Centaurea L. Taxa (Asteraceae) from Turkey. Turk J Bot 33: 97-104.
Mathew BF (1982). The Crocus, A Revision of The Genus Crocus (Iridaceae), B.T. Batsford Ltd. London.
Mathew BF (1984). Crocus L. In: Davis PH (ed.), Flora of Turkey and the East Aegean Islands, Vol. 8, pp. 413-438, Edinburgh: Edinburgh University Press.
Mathew BF (1988). Crocus L. In: Davis PH, Mill RR & Tan K (eds.) Flora of Turkey and the East Aegean Islands (Suppl. 1), Vol. p. 10, 228, Edinburgh: Edinburgh University Press.
Mathew BF (2000). Crocus L. In: Güner A, Özhatay, N, Ekim T & Baser, KHC, (eds.), Flora of Turkey and the East Aegean Islands (Suppl. 2), Vol. 11, pp. 271–274, Edinburgh: Edinburgh University Press.
Özdemir C, Akyol Y & Alçıtepe E (2004). Morphological and anatomical studies on two endemic Crocus species of Turkey area. Pak J Bot 36: 103–113.
Özdemir C, Baran P & Akyol Y (2005). The Morphology and Anatomy of Crocus flavus Weston subsp. flavus (Iridaceae). Turk J Bot 30: 175-180.
Özhatay N, Kültür Ş & Aslan S (2009). Check-List of Additional Taxa To The Supplement Flora of Turkey IV. Turk J Bot 33: 191-226.
Pasche E (1994a). A new Crocus (Iridaceae) from Turkey. Herbertia 49: 67-75.
Pasche E (1994b). Uber einige Crocus and Iris Arten Anatoliens. Stapfia 34: 89-102.
Petersen G, Seberg O, Thorsİe S, Jİrgensen T & Mathew B (2008). A phylogeny of the genus Crocus (Iridaceae) based on sequence data from five plastid regions. Taxon 57 (2): 487-499.
Pınar NM, Ekici M, Aytac Z, Akan H, Çeter T & Alan Ş (2009a). Pollen morphology of Astragalus L. sect. Onobrychoidei DC. (Fabaceae) in Turkey. Turk J Bot 33: 291-303.
Pınar NM, Duran A, Çeter T & Tuğ GN (2009b) Pollen and Seed Morphology of the Genus Hesperis L. (Brassicaceae) in Turkey. Turk J Bot 33: 83-96.
Pulido L, Gattuso S & Gattuso M (2004). Comparative Morphoanatomical Study of Three Species Pertaining to the Crocus Genus: C. nevadensis, C. nudiflorus and C. sativus Differentiating Characteristics. Proceedings of the I. International Symposium on Saffron Biology and Biotechnology-22-25 October 2003, Albacete-Spain. Acta Hort 650: 59-65.
Rodriguez A & Catedral LO (2003). Colima (Tigridieae:Iridaceae), A new genus from western Mexico and a new species: Colima tuitensis from Jalisco. Acta Bot Mexicana 65: 51-60.
Rudall P (1993). Anatomy and systematics of Iridaceae. Bot J Linn Soc 114: 1-21.
Rudall P & Mathew B (1990). Leaf anatomy in Crocus (Iridaceae). Kew Bull 45(3): 535-544.
Satıl F & Selvi S (2007). An anatomical and ecological study of some Crocus L. taxa (Iridaceae) from the west part of Turkey. Acta Bot Croat 66: 25-33.
Seberg O & Petersen G (2009). How many loci does it take to DNA barcode a Crocus? PLoS ONE 4(2): e4598. 1-6.
Shorina NI (1975). Leaf structure in some saffrons in association with the evolution of the genus Crocus L. Byull. Mosk. Obsch. Ispyt. Prir., Otdel Bio 80: 117-125.
Sık L, Candan F, Soya S, Karamenderes C, Kesercioglu T & Tanyolac B (2008). Genetic Variation Among Crocus L. Species from Western Turkey as Revealed by RAPD and ISSR Markers. JABS 2 (2): 73- 78.
Swofford D (2001). PAUP. Phylogenetic Analysis Using Parsimony and Other Methods, 4.0b10 32-bit for Windows Preliminary –beta- test version. Smithsonian Institution, Sinauer Associates, Inc., 23 Plumtree Rd. Sunderland, MA 01375-0407 U.S.A.