Bengi Baba ERDAĞ, Yelda Çalmaz EMEK, Serap Kurt AYDOĞAN

Clonal propagation of Dorystoechas hastata via axillary shoot proliferation

A protocol for the in vitro seed germination, seedling development, and axillary shoot propagation of Dorystoechas hastata Boiss. & Heldr. ex Bentham is described. By employing sterilised seeds that were transferred onto in vitro media, the effects of various in vitro media, photoperiods, and temperatures on germination were investigated. At the end of these experiments, a final protocol was determined for the in vitro germination of D. hastata seeds. With this protocol, the highest germination rate was obtained with distilled water supplemented with vitamins and 1 mg L-1 of gibberellic acid (GA3), with a 16:8 photoperiod at 24 ± 2 °C. Germinated seeds were transferred onto various in vitro media for further seedling development, and the appropiate medium for seedling development was found to be half-strength Murashige and Skoog medium (MS). In axillary shoot propagation experiments, the highest shoot number per explant was obtained with half-strength MS medium supplemented with 0.5 mg L-1 of N6-Benzyladenine (BA). Solitary shoots, removed from stock cultures, were transferred onto half-strength MS media supplemented with various concentrations of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) for rooting. The maximum rooting rate was obtained with half-strength MS medium supplemented with 1 mg L-1 of IBA. Rooted plantlets were gradually acclimatised to the external environment.

Anahtar Kelimeler:

Key words: Dorystoechas hastata, endemic species, in vitro, germination, propagation

Clonal propagation of Dorystoechas hastata via axillary shoot proliferation

Keywords:

Key words: Dorystoechas hastata, endemic species, in vitro, germination, propagation,

PDF

___

Agrawal PK, Karihaloo JL, Ahmed SMM & Gupta PC (1973). Predicting germinability in maize, wheat and paddy on the basis of tetrazolium test. Seed Res 1: 83- 85.
Arıkat NA, Jawad FM, Karam NS & Shibli RA (2004). Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill.). Sci Hortic 100: 193-202.
Başer KHC & Öztürk N (1992). Composition of the essential oil of Dorystoechas hastata, a monotypic endemic from Turkey. J. Essent. Oil Res 4: 369-374.
Baytop T (1999). Türkiye’de Bitkiler ile Tedavi (Geçmişte ve Bugün), p. 194. 2nd ed. İstanbul: Nobel Tıp Kitabevi.
Biddington NL (1992). The influence of ethylene in plant tissue culture. Plant Growth Regul 11: 173-187.
Elangomathavan R, Prakash S, Kathiravan K, Seshadri S & Ignacimuthu S (2003). High frequency in vitro propagation of Kidney Tea Plant. Plant Cell Tiss Org 72: 83-86.
Etkin NL (1998). Indigenous patterns of conserving biodiversity: pharmacologic implications. J Ethnopharmacol 63: 233-245.
Fay MF (1994). In what situation is in vitro culture appropriate to plant conservation? Biodivers Conserv 3: 176-183.
Fracaro F & Echeverrigaray S (2001). Micropropagation of Cunila galioides, a popular medicinal plant of South Brazil. Plant Cell Tiss Org 64: 1-4.
Gamborg OL, Miller RA & Ojima K (1968). Nutrition requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-159.
Karagözler AA, Erdağ B, Emek YÇ & Uygun DA (2008). Antioxidant activity and proline content of leaf extracts from Dorystoechas hastata. Food Chem 111: 400-407.
Kauffman MR (1969). Effects of water potential of germination of lettuce, sunflower and citrus seeds. Can J Bot 49: 410-515.
Kermode AR (1990). Regulatory mechanisms involved in the transition from seed development to germination. Critical Rev Plant Sci 9:155-195.
Kermode AR (1995). Regulatory mechanisms in the transition from seed development to germination: Interaction between the embryo and the seed environment. In: Kigel J, Galili G (eds.) Seed Development and Germination, pp. 273- 332. New York: Marcel Dekker Inc.
Khan AA (1977). The physiology and biochemistry of seed dormancy and germination. North-Holland: Amsterdam.
Khan MR, Rashid H & Quaraishi A (2002). In vitro shoot development from juvenile cuttings of field-grown olive (Olea europaea L.) cv. Leccino. Journal of Biological Sciences 2: 438- 440.
Lai-Keng C & Leng W (2004). Plant regeneration from stem nodal segments of Orthosiphon stamineus Benth., a medicinal plant with diuretic activity. In vitro Cell Dev Biol Plant 40: 115-118.
Maliro MFA & Kwapata MB (2000). Apomictic embryo development and survival in Uapaca kirkiana under in vitro and in vivo seed germination. Sci Hortic 83: 139-147.
Muntz K (1982). Seed development. In: Parthier B, Boulter D (eds.) Nucleic Acids and Proteins in Plants, pp. 505-558. Berlin: Springer-Verlag.
Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473- 497.
Murch SJ, KrishnaRaj S & Saxena PK (2000). Phytomaceuticals: Mass production, standardization and conservation. Sci Rev Alternative Med 4: 39-43.
Padilla IMG & Encina CL (2003). In vitro germination of cherimoya (Annona cherimola Mill.) seeds. Sci Hortic 97: 219-227.
Pattnaik SK & Chand PK (1996). In vitro propagation of the medicinal herbs. Ocimum americanum L. syn, O. canum Sims. (hoary basil) and O. sanctum L. (holy basil). Plant Cell Rep 15: 846-850.
Pierik RLM (1987). In vitro culture of higher plants. Dordrecht, Netherlands: Martinus Nijhoff Publishers.
Sunandakumari C, Martin KP, Chithra M, Sini S & Madhusoodanan PV (2004). Rapid axillary bud proliferation and ex vitro rooting of herbal spice, Mentha piperita L. Indian J Biotechnol 3: 108- 112.
Tavares AC, Pimenta MC & Goncalves MT (1996). Micropropagation of Melissa officinalis L. through proliferation of axillary shoots. Plant Cell Rep 15: 441-444.
Thanos CA & Doussi MA (1995). Ecophysiology of seed germination in endemic labiates of Crete. Israel J Plant Sci 43: 227-237.
Ulubelen A, Meriçli AH & Meriçli F (2004). Diterpenes and norditerpenes from the roots of Dorystoechas hastata. Pharmazie 59: 301-303.
Ünal O, Gökçeoğlu M & Topçuoğlu F (2004). Antalya endemiği Origanum türlerinin tohum çimlenmesi ve çelikle çoğaltımı üzerinde araştırmalar. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 17: 135-147.
Valant-Vetschera KM, Roitman JN & Wollenweber E (2003). Chemodiversity of exudate flavonoids in some members of the Lamiaceae. Biochem Syst Ecol 31: 1279-1289.
Vieira RF & Skorupa LA (1993). Brazilian medicinal plants gene bank. Acta Hort 330: 51-58.
White P (1963). The Cultivation of Animal and Plant Cells. New York: Ronald Press.
Zielinski H, Frias J, Piskula MK & Kozlowska H (2006). The effect of germination process on the superoxide dismutase-like activity and thiamine, riboflavin and mineral contents of rape seeds. Food Chem 99: 516-520.