Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties
To identify and isolate genes related to abiotic stress (salinity and drought) tolerance in grapevine, a candidate gene approach led to the isolation from Cabernet Sauvignon cultivar of a full-length cDNA of dehydrin gene. The latter, named VvDhn, which is highly and mainly induced in late embryogenesis in seeds, encodes for a protein of 124 amino acids with a predicted molecular mass of 13.3 kDa. Details of the physicochemical parameters and structural properties (molecular mass, secondary structure, conserved domains and motives, and putative posttranslational modification sites) of the encoded protein have also been elucidated. The expression study of VvDhn was carried out within plant organs and tissues as well as under drought and salt stresses. VvDhn was not detected in vegetative tissue, whereas it was only expressed during seed development (during late embryogenesis) at extremely high levels and was induced by salt and drought stresses as well as ABA application. Moreover, salt stress induced VvDhn expression in the tolerant variety (Razegui) but not the sensitive variety (Syrah), which did not display expression variation during stress; VvDhn expression level and salt-stress response depend on regulatory mechanisms that are efficient only in the tolerant variety. On the other hand, under drought stress VvDhn was induced in both tolerant and sensitive varieties, with higher levels in the tolerant variety. In addition, stress signal molecules such as ABA (applied alone or in combination with saccharose) induced VvDhn expression, even at low levels. Minimal knowledge about the role and functionality of this gene is necessary and constitutes a prerequisite for including VvDhn in grapevine abiotic stress tolerance improvement programs.
Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties
To identify and isolate genes related to abiotic stress (salinity and drought) tolerance in grapevine, a candidate gene approach led to the isolation from Cabernet Sauvignon cultivar of a full-length cDNA of dehydrin gene. The latter, named VvDhn, which is highly and mainly induced in late embryogenesis in seeds, encodes for a protein of 124 amino acids with a predicted molecular mass of 13.3 kDa. Details of the physicochemical parameters and structural properties (molecular mass, secondary structure, conserved domains and motives, and putative posttranslational modification sites) of the encoded protein have also been elucidated. The expression study of VvDhn was carried out within plant organs and tissues as well as under drought and salt stresses. VvDhn was not detected in vegetative tissue, whereas it was only expressed during seed development (during late embryogenesis) at extremely high levels and was induced by salt and drought stresses as well as ABA application. Moreover, salt stress induced VvDhn expression in the tolerant variety (Razegui) but not the sensitive variety (Syrah), which did not display expression variation during stress; VvDhn expression level and salt-stress response depend on regulatory mechanisms that are efficient only in the tolerant variety. On the other hand, under drought stress VvDhn was induced in both tolerant and sensitive varieties, with higher levels in the tolerant variety. In addition, stress signal molecules such as ABA (applied alone or in combination with saccharose) induced VvDhn expression, even at low levels. Minimal knowledge about the role and functionality of this gene is necessary and constitutes a prerequisite for including VvDhn in grapevine abiotic stress tolerance improvement programs.
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- Allagulova ChR, Gilamov FR, Shakirova FM, Vakhitov VA (2003). The plant dehydrins: structure and functions. Biochemistry (Moscow) 68: 945–951.
- Alsheikh MK, Heyen BJ, Randall SK (2003). Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation. J Biol Chem 278: 40882–40889.
- Asif MH, Dhawan P, Nath P (2000). A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Mol Biol Rep 18: 109–115.
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403–410.
- Bailey TL, Elkan C (1994). Fitting a mixture model by expectation maximization to discover motifs in biopolymers, Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28–36, AAAI Press, Menlo Park, California, USA.
- Blaskovic S, Blanc M, van der Goot FG (2013). What does S-palmitoylation do to the membrane proteins? FEBS J 280: 2766–2774.
- Campbell SA, Close TJ (1997). Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytol 137: 61–74. Chang S, Puryear J, Cairney J (1993). A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11: 113– 116.
- Chitarra W, Balestrini R, Vitali M, Pagliarani C, Perrone I, Schubert A, Lovisolo C (2014). Gene expression in vessel-associated cells upon xylem embolism repair in Vitis vinifera L. petioles. Planta 239: 887–899.
- Choi Y-J, Hur YY, Jung S-M, Kim S-H, Noh J-H, Park S-J, Park K-S, Yun H-K (2013). Transcriptional analysis of Dehydrin1 genes responsive to dehydrating stress in grapevines. Hortic Environ Biote 54: 272–279.
- Church GM, Gilbert W (1984). Genomic sequencing. P Natl Acad Sci USA 81: 1991–1995.
- Close TJ (1996). Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plantarum 97: 795–803.
- Close TJ (1997). Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiol Plantarum 100: 291–296.
- Cramer G (2010). Abiotic stress and plant responses from the whole vine to the genes. Aust J Grape Wine R 16: 86–93.
- Cramer GR, Van Sluyter SC, Hopper DW, Pascovici D, Keighley T, Haynes PA (2013). Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera L.) in response to water deficit. BMC Plant Biol 13: 49.
- Daldoul S, Chenenanoui S, Mliki A, Höfer M (2009). Improvement of an RNA purification method for grapevine (Vitis vinifera L.) suitable for cDNA library construction. Acta Physiol Plant 31: 871–875.
- Allagulova ChR, Gilamov FR, Shakirova FM, Vakhitov VA (2003). The plant dehydrins: structure and functions. Biochemistry (Moscow) 68: 945–951.
- Alsheikh MK, Heyen BJ, Randall SK (2003). Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation. J Biol Chem 278: 40882–40889.
- Asif MH, Dhawan P, Nath P (2000). A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Mol Biol Rep 18: 109–115.
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403–410.
- Bailey TL, Elkan C (1994). Fitting a mixture model by expectation maximization to discover motifs in biopolymers, Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28–36, AAAI Press, Menlo Park, California, USA.
- Blaskovic S, Blanc M, van der Goot FG (2013). What does S-palmitoylation do to the membrane proteins? FEBS J 280: 2766–2774.
- Campbell SA, Close TJ (1997). Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytol 137: 61–74.
- Chang S, Puryear J, Cairney J (1993). A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11: 113– 116.
- Chitarra W, Balestrini R, Vitali M, Pagliarani C, Perrone I, Schubert A, Lovisolo C (2014). Gene expression in vessel-associated cells upon xylem embolism repair in Vitis vinifera L. petioles. Planta 239: 887–899.
- Choi Y-J, Hur YY, Jung S-M, Kim S-H, Noh J-H, Park S-J, Park K-S, Yun H-K (2013). Transcriptional analysis of Dehydrin1 genes responsive to dehydrating stress in grapevines. Hortic Environ Biote 54: 272–279.
- Church GM, Gilbert W (1984). Genomic sequencing. P Natl Acad Sci USA 81: 1991–1995.
- Close TJ (1996). Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plantarum 97: 795–803.
- Close TJ (1997). Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiol Plantarum 100: 291–296.
- Cramer G (2010). Abiotic stress and plant responses from the whole vine to the genes. Aust J Grape Wine R 16: 86–93.
- Cramer GR, Van Sluyter SC, Hopper DW, Pascovici D, Keighley T, Haynes PA (2013). Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera L.) in response to water deficit. BMC Plant Biol 13: 49.
- Daldoul S, Chenenanoui S, Mliki A, Höfer M (2009). Improvement of an RNA purification method for grapevine (Vitis vinifera L.) suitable for cDNA library construction. Acta Physiol Plant 31: 871–875.
- Sadder MT, Al-Doss AA (2014). Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus). Turk J Biol 38: 469–477.
- Schwarz F, Aebi M (2011). Mechanisms and principles of N-linked protein glycosylation. Curr Opin Struc Biol 21: 576–582. Seymour GB, Granell A (2014). Fruit development and ripening. J Exp Bot 65: 4489–4490.
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar K (2005). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739.
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997). The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.
- Vaseva II, Anders I, Feller U (2014a). Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens. J Plant Physiol 171: 213–224.
- Vaseva II, Anders I, Yuperlieva-Mateeva B, Nenkova R, Kostadinova A, Feller U (2014b). Dehydrin expression as a potential diagnostic tool for cold stress in white clover. Plant Physiol Bioch 78: 43–48.
- Wang Y, Dasso M (2009). SUMOylation and deSUMOylation at a glance. J Cell Sci 122: 4249–4252.
- Wang Y, Xu H, Zhu H, Tao Y, Zhang G, Zhang L, Zhang C, Zhang Z, Ma Z (2014). Classification and expression diversification of wheat dehydrin genes. Plant Sci 214: 113–120.
- Xiao H, Nassuth A (2006). Stress- and development-induced expression of spliced and unspliced transcripts from two highly similar dehydrin 1 genes in V. riparia and V. vinifera. Plant Cell Rep 25: 968–977.
- Yang Y, He M, Zhu Z, Li S, Xu Y, Zhang C, Singer SD, Wang Y (2012). Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. BMC Plant Biol 12: 140.
- Zhang HM, Zhang LS, Liu L, Zhu WN, Yang WB (2013). Changes of dehydrin profiles induced by drought in winter wheat at different developmental stages. Biol Plantarum 57: 797–800.