Comparative morphological, epidermal, and anatomical studies of Pinus roxburghii needles at different altitudes in the North-West Indian Himalayas

The aim of the present study was to understand the ecological adaptation of Pinus roxburghii Sarg. in the North-West Himalayan region. P. roxburghii needles showed morphological, epidermal, and anatomical variation at different altitudes. Needle length was negatively correlated with altitude. Stomatal characters like stomatal density, stomatal index, and guard cell lengths were found to be affected by environmental factors and showed a direct correlation with altitude. The results showed that potential conductance index was dependent on the climatic conditions of the habitat. The anatomical properties of needles exhibited variation from lower to higher elevation, especially in the number and position of resin ducts.

Comparative morphological, epidermal, and anatomical studies of Pinus roxburghii needles at different altitudes in the North-West Indian Himalayas

The aim of the present study was to understand the ecological adaptation of Pinus roxburghii Sarg. in the North-West Himalayan region. P. roxburghii needles showed morphological, epidermal, and anatomical variation at different altitudes. Needle length was negatively correlated with altitude. Stomatal characters like stomatal density, stomatal index, and guard cell lengths were found to be affected by environmental factors and showed a direct correlation with altitude. The results showed that potential conductance index was dependent on the climatic conditions of the habitat. The anatomical properties of needles exhibited variation from lower to higher elevation, especially in the number and position of resin ducts.

___

  • Armstrong JK, Williams K, Huenneke LF & Mooney HA (1988). Topographic position effects on growth depression of California (USA) Sierra Nevada pines during the 1982–1983 El Nino. Arctic and Alpine Research 20: 352–357.
  • Beerling DJ, Birks HH & Woodward FI (1995). Rapid late-glacial atmospheric CO 2 changes reconstructed from the stomatal density record of fossil leaves. Journal of Quaternary Sciences 10: 379 – 3
  • Beerling DJ & Kelly CK (1996). Evolutionary comparative analysis of the relationship between leaf structure and function. New Phytologist 134: 35 –
  • Biswas C & Johri BM (1997). The Gymnosperms. New York: SpringerVerlag.
  • Coupe SA, Palmer BG, Lake JA, Overy SA, Oxborough K, Woodward FI, Gray JE & Quick WP (2006). Systemic signalling of environmental cues in Arabidopsis leaves. Journal of Experimental Botany 57: 329–341.
  • Eo JK (2012). A simple technique for cross-sectioning Gymnosperm needle leaves using microtome. Turkish Journal of Botany 36: 213–216.
  • Fabbro T & Körner Ch (2004). Altitudinal differences in flower traits and reproductive allocation. Flora 199: 70–81.
  • Fahn A & Benayoun J (1976). Ultrastructure of resin ducts in Pinus halepensis development, possible sites of resin synthesis, and mode of its elimination from the protoplast. Annuals of Botany 40: 857–863.
  • Friend AD & Woodward FI (1990). Evolutionary and ecophysiological responses of mountain plants to the growing season environment. Advances in Ecological Research 20: 59– 1
  • Gray JE, Holroyd GH, van der Lee F, Sijmons PC, Woodward FI, Schuch W & Hetherington AM (2000). HIC: a gene involved in controlling stomatal development in responses to changes in atmospheric CO 2 concentration. Nature 408: 713–715.
  • Helmers AE (1943). Ecological anatomy of ponderosa pine needles. The American Midland Naturalist 29: 55–71.
  • Hetherington AM & Woodward FI (2003). The role of stomata in sensing and driving environmental change. Nature 424: 901– 90
  • Hoch G & Körner Ch (2003). The carbon charging of pines at the climatic treeline: a global comparison. Oecologia 135: 10–21.
  • Holland N & Richardson AD (2009). Stomatal length correlates with elevation of growth in four temperate species. Journal of Sustainable Forestry 28: 63–73.
  • He X-Q, Lin Y-H & Lin J-X (1998). Research on correlation between stomatal density and variation of atmospheric carbon dioxide during a century. Chinese Science Bulletin 43: 860–862 (in Chinese).
  • Hultine KR & Marshall JD (2001). A comparison of three methods for determining the stomatal density of pine needles. Journal of Experimental Botany 52: 369–373.
  • James JC, Grace J & Hoad SP (1994). Growth and photosynthesis of Pinus sylvestris at its altitudinal limit in Scotland. Journal of Ecology 82: 297–306.
  • Johansen DA (1940). Plant Microtechnique. New York: McGraw-Hill Book Co.
  • Kajimoto T (1993). Shoot dynamics of Pinus pumila in relation to altitudinal and wind exposure gradients on the Kiso mountain range, central Japan. Tree Physiology 13: 41–53.
  • Klimes L (2003). Life-forms and clonality of vascular plants along an altitudinal gradient in East Ladakh (NW Himalayas). Basic and Applied Ecology 4: 317–328.
  • Körner C (1988). Does global increase of CO 2 alter stomatal density? Flora 181: 253–257.
  • Körner C (1999). Alpine Plant Life. Berlin: Springer-Verlag.
  • Körner C (2007). The use of ‘altitude’ in ecological research. Trends in Ecology and Evolution 22: 11.
  • Körner C & Cochrane O (1985). Stomatal responses and water relations of Eucalyptus pauciflora in summer along an elevational gradient. Oecologia 74: 443–445.
  • Kouwenberg LLR, McElwain JC, Kürschner WM, Wagner F, Beerling DJ, Mayle FE & Visscher H (2003). Stomatal frequency adjustment of four conifer species to historical changes in atmospheric CO 2
  • . American Journal of Botany 90: 610–619 Lin J, Jach ME & Ceulemans R (2001). Stomatal density and needle anatomy of Scots pine (Pinus sylvestris) are affected by elevated CO 2
  • . New Phytologist 150: 665–674 Lockheart MJ, Poole I, Van Bergen PF & Evershed RP (1998). Leaf carbon isotope composition and stomatal characters: important consideration for palaeoclimate reconstructions. Organic Geochemistry 29: 1003–1008.
  • Luo TX, Pan Y, Ouyang H, Shi P, Luo J, Yu Z & Lu Q (2004). Leaf area index and net primary productivity along subtropical to alpine gradients in the Tibetan Plateau. Global Ecology and Biogeography 13: 345–358.
  • McElwain JC (2004). Climate-independent paleoaltimetry using stomatal density in fossil leaves as a proxy for CO 2 partial pressure. Geology 32: 1017–1020.
  • Matziris DI (1984). Genetic variation in morphological and anatomical needle characteristic in the Black pine of Peloponnesos. Silvae Genetica 33: 4–5.
  • Mehra PN (1988). Indian Conifer, Gnetophytes and Phylogeny of Gymnosperms. New Delhi: Rajbandhu.
  • Nagy L, Grabherr G & Körner C (2003). Alpine biodiversity in space and time: a synthesis. Alpine Biodiversity in Europe 167: 453– 4
  • Napp-Zinn K (1966). Anatomie des Blattes. I. Blattanatomie der Gymnospermen. Berlin-Nikolassee: Gebrüdder Borntraeger.
  • Overhulsen D & Cara RI (1981). Occluded resin canals associated with egg cavities made by shoot infecting Pissodes. Forest Science 27: 297–298.
  • Qiang W, Wang X, Chen T, Feng H, An L, He Y & Wang G (2003). Variations of stomatal density and carbon isotope values of Picea crassifolia at different altitudes in the Qilian Mountains. Trees 17: 258–262.
  • Reisch C, Anke A & Rohl M (2005). Molecular variation within and between ten populations of Primula farinosa (Primulaceae) along an altitudinal gradient in the northern Alps. Basic of Applied Ecology 6: 35–45.
  • Royer DL, Wing SL, Beerling DJ, Jolley DW, Koch PL, Hickey LJ & Berner RA (2001). Paleobotanical evidence for near-presentday levels of atmospheric CO 2 during part of the Tertiary. Science 292: 2310–2313.
  • Schoettle AW (1990). The interaction between leaf longevity and shoot growth and foliar biomass per shoot in Pinus contorta at two elevations. Tree Physiology 7: 209–214.
  • Schoettle AW & Rochelle SG (2000). Morphological variation of Pinus flexilis (Pinaceae), a bird-dispersed pine, across a range of elevations. American Journal of Botany 87: 1797–1806.
  • Sheue CR, Yang YP & Huang LLK (2003). Altitudinal variation of resin ducts in Pinus taiwanensis Hayata (Pinaceae) needles. Botanical Bulletin Academia Sinica 44: 305–313.
  • Van de Water PK, Leavitt SW & Betancourt JL (1994). Trends in stomatal density and 13C/12C ratio of Pinus flexilis needles during last glacial-interglacial cycle. Science 264: 239–243.
  • Woodward FI (1987). Stomatal numbers are sensitive to increases in CO 2 from pre-industrial levels. Nature 327: 617–618.
  • Woodward FI & Bazzaz FA (1988). The response of stomatal density to CO 2 partial pressure. Journal of Experimental Botany 39: 1771–1781.