Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding
Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium (DKW) with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the `OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in `OV 17? and `OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA.
Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding
Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium (DKW) with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the `OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in `OV 17? and `OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA.
___
- Bagi F, Bese G, Budakov D, Ognjanov V, Ljubojević M, Váczi A, Stojšin V (2012). Testing of rootstocks for sweet cherry for prune dwarf virus. Plant doctor 40: 498–502.
- Borkowska B (1986). Dormancy of sour cherry micropropagated plantlets. Tree Physiol 1: 303–307.
- Bošnjaković D, Ognjanov V, Ljubojević M, Barać G, Predojević M, Mladenović E, Čukanović J (2012). Biodiversity of wild fruit species of Serbia. Genetika–Serbia 44: 81–90.
- Bošnjaković D, Ognjanov V, Barać G, Ljubojević M, Pranjić A, Dugalić K (2013). Micropropagation of low-vigorous rootstock selections for sweet and sour cherry. Journal of Pomology 47: 121–128 (article in Serbian with an abstract in English).
- Cerović R, Ružić İ (1987). Micropropagation of sour cherry (Prunus cerasus L.) cv. Šumadinka. Plant Cell Tiss Org 9: 151–157.
- Dorić D, Ognjanov V, Ljubojević M, Barać G, Dulić J, Pranjić A, Dugalić K (2014). Rapid propagation of sweet and sour cherry rootstocks. Not Bot Horti Agrobo 42: 488–494.
- Dradi G, Vito G, Standardi A (1996). In vitro mass propagation of eleven Prunus mahaleb ecotypes. Acta Hort 410: 477–484.
- Driver JA, Kuniyuki AH (1984). In vitro propagation of Paradox walnut rootstock. Hortscience 19: 507–509.
- Druart P (2013). Micropropagation of Prunus species relevant to cherry fruit production. In: Lambardi M, Ozudogru EA, Jain SM, editors. Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. New York, NY, USA: Humana Press, pp. 119–136.
- Hossini AD, Moghadam EG, Anahid S (2010). Effects of medium cultures and plant grow regulators in micropropagation of Gisela 6 rootstock. Ann Biol Res 1: 135–141.
- Mahdavian M, Bouzari N, Abdollahi H (2011). Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Biharean Biologist 5: 86–90.
- Miletić R, Žikić M, Mitić N, Nikolić R (2008). Identification and in vitro propagation of promising ‘Oblačinska’ sour cherry selections in eastern Serbia. Acta Hort 795: 159–162.
- Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plantarum 15: 473–442.
- Nikolić D, Rakonjac V, Milutinović M, Fotirić M (2005). Genetic divergence of Oblačinska sour cherry (Prunus cerasus L.) clones. Genetika–Serbia 37: 191–198.
- Ognjanov V, Keserović Z, Mratinić E, Miletić R (2009). Fruit germplasm in Serbia. In: Proceedings of the Scientific Meeting on the Management of Genetic Resources of Plant and Animal Species in Serbia. Belgrade, Serbia, p. 14.
- Ognjanov V, Ljubojević M, Ninić–Todorović J, Bošnjaković D, Barać G, Čukanović J, Mladenović E (2012). Morphometric diversity of dwarf sour cherry germplasm in Serbia. J Hortic Sci Biotech 87: 117–122.
- Preece J (2008). Stock plant physiological factors affecting growth and morphogenesis In: George FE, Hall AM, De Klerk GJ, editors. Plant Propagation by Tissue Culture. 3rd ed. The Netherlands: Springer, p. 419.
- Pruski K, Astatkie T, Nowak J (2005). Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus tomentosa L.). Plant Cell Tiss Org 82: 207–211.
- Radičević S, Cerović R, Lukić M, Paunović S, Jevremović D, Milenković S, Mitrović M (2012). Selection of autochthonous sour cherry (Prunus cerasus L.) genotypes in Feketić region. Genetika–Serbia 44: 285–297.
- Ružić İ, Cerović R (2002). The application of fruit micropropagation in vitro for commercial purposes. Proceedings of Research Papers Institut PKB Agroekonomik 8: 213–224 (article in Serbian with an abstract in English).
- Ružić İ, Cerović R (2003). The application of in vitro methods for stone fruit species. Journal of Pomology 37: 37–49 (article in Serbian with an abstract in English).
- Ružić İ, Vujović T, Cerović R, Radičević S (2007). Low-vigorous sweet and sour cherry rootstock Damil GM 61/1—possibility for rapid propagation and introduction into production. Proceedings of Research Papers Institut PKB Agroekonomik 13: 47–55 (article in Serbian with an abstract in English).
- Saponari M, Bottalico G, Savino G (1999). In vitro propagation of Prunus mahaleb and its sanitation from Prune dwarf virus. Advances in Horticultural Science 13: 56–60.
- Scaltsoyiannes A, Tsoulpha P, Iliev I, Theriou K, Tsaktsira M, Mitras D, Karanikas C, Mahmout S, Christopoulus V, Scaltsoyiannes V et al. (2009). Vegetative propagation of ornamental genotypes of Prunus avium L. Propag Ornam Plants 9: 198–206.
- Schenk RU, Hildebrandt AC (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204.
- Sedlak J, Paprštein F, Erbenova M (2008). In vitro propagation of PHL dwarfing cherry rootstocks. Acta Hort 795: 395–400.
- Sedlak J, Paprštein F (2013). In vitro multiplication and rooting of two Czech sweet cherry cultivars. In: Proceeding of the Seventh Cherry International Symposium. Plasencia, Spain, p. 118.
- Singh SR, Sundouri AS, Sharma MK, Srivastava KK, Dar HA (2010). Proliferation and rooting efficiency studies in sour cherry (Prunus cerasus) using in vitro techniques. J Hortic Sci 5: 48– 52.
- Vujović T, Ružić İ, Cerović R (2009). The influence of imidazole fungicide on multiplication in vitro of low-vigorous sweet cherry rootstock Gisela 6. Journal of Pomology 43: 87–93 (article in Serbian with an abstract in English).
- Vujović T, Ružić İ, Cerović R (2012). In vitro multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Hortic Sci 39: 101–107.