Mine TÜRKTAŞ, Meral ASLAY, Erdal KAYA, Fahriye ERTUĞRUL

Molecular characterization of phylogeneticrelationships in Fritillaria species inferred from chloroplast trnL-trnF sequences

The genus Fritillaria embraces about 165 taxa in the family Liliaceae. In this study, the molecular phylogenetic relationships between 10 Fritillaria taxa were presented. Fritillaria spp. were collected from their natural habitats, and specimens with different morphological features were obtained via selective breeding. Specimens with the desired characters were presented as promising candidate cultivars for ornamental use. Phylogenetic analysis was based on DNA sequences of the chloroplast trnL-trnF region. The phylogeny was constructed using the neighbor joining, maximum parsimony, maximum likelihood, and Bayesian inference methods. The data showed that the examined Fritillaria spp. were evidently diverged into 2 Fritillaria subgenera. Members of the subgenus Fritillaria formed one clade while the other clade contained the subgenera Theresia and Petilium. Separation within the latter clade was strongly supported with bootstrap values, whereas resolution within the subgenus Fritillaria remained ambiguous. This analysis revealed the feasibility of the chloroplast trnL-trnF region DNA sequence for phylogeny of the Fritillaria species. Our study is the first phylogenetic analysis in Fritillaria spp. based on the trnL-trnF region. Fingerprint data of these cultivars would be a valuable source for their identification and for the generation of new cultivars in future.

Anahtar Kelimeler:

Key words: Fritillaria, trnL, trnF, intergenic spacer, chloroplast, phylogeny, ornamental plants

Molecular characterization of phylogeneticrelationships in Fritillaria species inferred from chloroplast trnL-trnF sequences

Keywords:

Key words: Fritillaria, trnL, trnF, intergenic spacer, chloroplast, phylogeny, ornamental plants,

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Özhatay Fritillaria L. In: Güner A, Özhatay N, Ekim T et N. al. eds. Flora of Turkey and the East Aegean Islands (Suppl. 2). Edinburgh University Press. 2000: pp. 243-246.
Özhatay N, Kültür Ş. Check-list of additional taxa to the supplement Flora of Turkey III. Turk J Bot 30: 281-316, 2006. 3. Özhatay N, Kültür Ş, Aslan S. Check-list of additional taxa to the supplement Flora of Turkey IV. Turk J Bot 33: 191-226, 2009.
Özler H, Pehlivan S. Comparison of pollen morphological structures of some taxa belonging to Asparagus L. and Fritillaria L. (Liliaceae) from Turkey. Bangladesh J Bot 36: 111-120, 2007. 5. Tekşen M, Aytaç Z. Th e revision of the genus Fritillaria L. (Liliaceae) in the Mediterranean region (Turkey). Turk J Bot 35: 447-478, 2011. 6. Tekşen M, Aytaç Z. New Fritillaria L. taxa from Turkey. Isr J Plant Sci 52: 347-355, 2004. 7. Zhang QJ, Zheng ZF, Yu DQ. Steroidal alkaloids from the bulbs of Fritillaria unibracteata. J Asian Nat Prod Res 13: 1098-1103, 2011.
Zhou JL, Xin GZ, Shi ZQ et al. Characterization and identifi cation of steroidal alkaloids in Fritillaria species using liquid chromatography coupled with electrospray ionization quadrupole time-of-fl ight tandem mass spectrometry. J Chromatogr A 1217: 7109-7122, 2010. 9. Linnaeus C. Species Plantarum. 303-304, 1753.
Baker JG. Revision of the genera and species of Tulipeae. J Linn Soc 14: 211-310, 1874.
Bentham G, Hooker, JD. Fritillaria. Genera Plantarum 3: 817- 818, 1883.
Boissier Fritillaria. Flora Orientalis 5: 176-190, 1882. PE.
Turrill WB, Sealy JR. Studies in the genus Fritillaria (Liliaceae). Hooker’s Icon Pl 3: 1-2, 1980.
Rix M, Frank E, Webster G. Fritillaria: A Revised Classifi cation: Together with an Updated List of Species. Th e Fritillaria Group of the Alpine Garden Society. Berkshire, United Kingdom; 2001.
Fay MF, Chase MW. Modern concepts of Liliaceae with a focus on the relationships of Fritillaria. Curtis’s Bot Mag 17: 146-149, 2000.
Rİnsted N, Law S, Th ornton H et al. Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classifi cation of Fritillaria. Mol Phlyogenet Evol 35: 509-527, 2005.
Khaniki GB. Fruit and seed morphology in Iranian species of Fritillaria subgenus Fritillaria (Liliaceae). Pakistan J Bot 35: 313-322, 2003.
Khaniki GB, Persson K. Nectary morphology in south west Asian Fritillaria (Liliaceae). Nord J Bot 17: 579-611, 1997.
Kosenko VN. Contributions to the pollen morphology and taxonomy of the Liliaceae. Grana 38: 20-30, 1999.
Tekşen M, Aytaç Z, Pınar NM. Pollen morphology of the genus Fritillaria L. (Liliaceae) in Turkey. Turk J Bot 34: 397-416, 2010.
Peruzzi L, Leitch IJ, Caparelli KF. Chromosome diversity and evolution in Liliaceae. Ann Bot-London 103: 459-475, 2009.
Fay MF, Chase MW, Rİnsted N et al. Phylogenetics of Liliales: summarized evidence from combined analyses of fi ve plastid and one mitochondrial Loci. Aliso 22: 559-565, 2006.
Guo SQ, Xiong M, Ji CF et al. Molecular phylogenetic reconstruction of Osmanthus Lour. (Oleaceae) and related genera based on three chloroplast intergenic spacers. Plant Syst Evol 294: 57-64, 2011.
Peterson A, John H, Koch E et al. A molecular phylogeny of the genus Gagea (Liliaceae) in Germany inferred from non-coding chloroplast and nuclear DNA sequences. Plant Syst Evol 245: 145-162, 2004.
Sang T, Crawford DJ, Stuessy TF. Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am J Bot 84: 1120-1136, 1997.
Drábková L, Kirschner J, Vlček Č et al. TrnL-trnF intergenic spacer and trnL intron defi ne major clades within Luzula and Juncus (Juncaceae): importance of structural mutations. J Mol Evol 59: 1-10, 2004.
Taberlet P, Gielly L, Pautou G et al. Universal primers for the amplifi cation of three non-coding regions of the chloroplast DNA. Plant Mol Biol 17: 1105-1109, 1991.
Hao DC, Huang BL, Chen SL et al. Evolution of the chloroplast trnL-trnF region in the gymnosperm lineages Taxaceae and Cephalotaxaceae. Biochem Genet 47: 351-369, 2009.
Zencirkıran M, Gürbüz İB. Turkish ornamental plants sector in the European Union screening process. J Fruit Ornam Plant Res 17: 235-250, 2009.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15, 1987.
Altschul SF, Gish W, Miller W et al. Basic local alignment search tool. J Mol Biol 215: 403-410, 1990.
Th ompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specifi c gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680, 1994.
Tamura K, Dudley J, Nei M et al. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) soft ware version 4.0. Mol Biol Evol 24: 1596-1599, 2007.
Felsentein J. Confi dence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791, 1985.
Ronquist F, Huelsenbeck JP. MRBAYES 3: bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574, 2003.
Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12: 357-358, 1996.
Zarrei M, Wilkin P, Fay MF et al. Molecular systematics of Gagea and Lloydia (Liliaceae; Liliales): implications of analyses of nuclear ribosomal and plastid DNA sequences for infrageneric classifi cation. Ann Bot-London 104: 125-142, 2009.
Kamenetsky R. Florogenesis in geophytes: classical and molecular approaches. Acta Hortic 886: 113-118, 2011.
Noy-Porat T, Flaishman MA, Eshel A et al. Florogenesis of the Mediterranean geophyte Narcissus tazetta and temperature requirements for fl ower initiation and diff erentiation. Sci Hortic-Amsterdam 120: 138-142, 2009.
Yoshida K, Ito D, Shinkai Y et al. Change of color and components in sepals of chameleon hydrangea during maturation and senescence. Phytochemistry 69: 3159-3165, 2008.
Alp Ş, Arslan N, Koyuncu M. Established forms of Fritillaria imperialis L. – A naturally growing species in Turkey. Pakistan J Bot 41: 1573-1576, 2009.
Khan IA, Azim MK. Variations in intergenic spacer rpl20-rps12 of mango (Mangifera indica) chloroplast DNA: implications for cultivar identifi cation and phylogenetic analysis. Plant Syst Evol 292: 249-255, 2011.
Khaniki GB. Chromosome number of Fritillaria subgenera Petilium and Th eresia (Liliaceae). Nucleus 45: 6-11, 2002.
İkinci N, Oberprieler C, Güner A. On the origin of European lilies: phylogenetic analysis of Lilium section Liriotypus (Liliaceae) using sequences of the nuclear ribosomal transcribed spacers. Willdenowia 36:647-656, 2006.
Sucher NJ, Tsim KW, Ip NY. Fritillaria species identifi cation. US Patent 6.569.625. Washington, DC: US Patent and Trademark Offi ce; 2003.