Genetic characterization of Turkish bread wheat landraces based on microsatellite markers and morphological characters

Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and 295 with 11 alleles, followed by Xgwm 261 and 325 with 9 alleles. The total number of alleles was 63, with an average number of 9 alleles. The dendrogram showed that the bread wheat landraces can be divided into 2 major groups. Based on matrix values, the closest genotypes of landraces were TR 63445 and TR 63886 for molecular data, and TR 37179 and TR 46797 for morphological data. On the other hand, the most genetically different genotypes were TR 37179 and TR 3608 in the SSR analysis, and TR 14851 and TR 3608 in the morphological characterization. These results showed that SSR markers and morphological characters could be successfully used in genetic characterization and genetic diversity in bread wheat landraces that may be useful for wheat breeding programs as genetic resources.

Genetic characterization of Turkish bread wheat landraces based on microsatellite markers and morphological characters

Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and 295 with 11 alleles, followed by Xgwm 261 and 325 with 9 alleles. The total number of alleles was 63, with an average number of 9 alleles. The dendrogram showed that the bread wheat landraces can be divided into 2 major groups. Based on matrix values, the closest genotypes of landraces were TR 63445 and TR 63886 for molecular data, and TR 37179 and TR 46797 for morphological data. On the other hand, the most genetically different genotypes were TR 37179 and TR 3608 in the SSR analysis, and TR 14851 and TR 3608 in the morphological characterization. These results showed that SSR markers and morphological characters could be successfully used in genetic characterization and genetic diversity in bread wheat landraces that may be useful for wheat breeding programs as genetic resources.

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  • Kahrizi D, Cheghamirza K, Kakaei M et al. Heritability and genetic gain of some morpho-physiological variables of durum wheat (Triticum turgidum var. durum). African J Biotechnol 9: 4687-4691, 2010.
  • Schut JW, Oi X, Stam P. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Th eor Appl Genet 95: 1161- 1168, 1997. 3. Marti J, Bort J, Slafer GA et al. Can wheat yield be assessed by early measurements of normalized diff erence vegetation index? Annal Appl Biol 150: 253-257, 2007.
  • Salem KFM, El-Zanaty AM, Esmail RM. Assessing wheat (Triticum aestivum L.) genetic diversity using morphological characters and microsatellite markers. World J Agri Sci 4: 538- 544, 2008.
  • Zarkti H, Ouabbou H, Hilali A et al. Detection of genetic diversity in Moroccan durum wheat accessions using agro- morphological traits and microsatellite markers. African J Agric Res 5: 1837-1844, 2010. 6. Akçura M. Th e relationships of some traits in Turkish winter bread wheat landraces. Turk J Agric For 35: 115-125, 2011. 7. Röder MS, Plaschke P, König SU et al. Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246: 327-333, 1995.
  • Hernández P, Laurie DA, Martin A et al. Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum. Th eor Appl Genet 104: 735-739, 2002. 9. Kandemir N, Yıldırım A, Gündüz R. Determining the levels of genetic variation using SSR markers in three Turkish barley materials known as Tokak. Turk J Agric For 34: 17-23, 2010.
  • Stachel M, Lelley T, Grausgruber H et al. Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic diff erentiation caused by selection for adaptation and use. Th eor Appl Genet 100: 242-248, 2000.
  • Dreisigacker S, Zhang P, Warburton ML et al. Genetic diversity among and within CIMMYT wheat landrace accessions investigated with SSRs and implications for plant genetic resources management. Crop Sci 45: 65-661, 2005.
  • Ijaz S, Khan IA. Molecular characterization of wheat germplasm using microsatellite markers. Genet Mol Res 8: 809-815, 2009.
  • Zhang P, Dreisigacker S, Buerkert A et al. Genetic diversity and relationships of wheat landraces from Oman investigated with SSR markers. Genet Res and Crop Evo 53: 1351-1360, 2006.
  • Iqbal N, Tabasum A, Sayed H et al. Evaluation of genetic diversity among bread wheat varieties and landraces of Pakistan by SSR markers. Cereal Res Commun 37: 489-498, 2009.
  • Mandoulakani BA, Bushehri AA, Tabatabaei BE et al. Genetic diversity among wheat cultivars using molecular markers. J Crop Improv 24: 299-309, 2010.
  • Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus 12: 13-15, 1990.
  • Röder MS, Korzun V, Wendehake K et al. A microsatellite map of wheat. Genetics 149: 2007-2023, 1998.
  • Rohlf FJ. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Version 1.70. Exeter Publications. New York; 1992.
  • Anderson JA, Churchill GA, Autrique JE et al. Optimizing parental selection for genetic linkage maps. Genome 36: 181- 186, 1993.
  • Eujayl I, Sorrels M, Baum M et al. Assessment of genotypic variation among cultivated durum wheat based on EST-SSRs and genomic SSRs. Euphytica 119: 39-43, 2001.
  • Huang XQ, Börner MS, Goral MW. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Th eor Appl Genet 105: 699-707, 2002.
  • Yildirim A, Ateş Sönmezoğlu Ö, Gökmen S et al. Determination of genetic diversity among Turkish durum wheat landraces by microsatellites. Afr J Biotechnol 10: 3915-3920, 2011.
  • Strelchenko P, Kenneth S, Mitrofanova O et al. Genetic diversity among hexaploid wheat landraces with diff erent geographical origins revealed by microsatellites: comparison with AFLP and RAPD. In: Proceedings of 4th International Crop Science Congress. Brisbane, Australia; 2004.
  • Wei YM, Hou YC, Yan ZH et al. Microsatellite DNA polymorphism divergence in Chinese wheat (Triticum aestivum L.) landraces highly resistant to Fusarium head blight. J Appl Genet 46: 3-9, 2005.
  • Allard RW, Bradshaw AD. Implications of genotype- environment interaction in applied plant breeding. Crop Sci 4: 503-508, 1964.
  • Zohary D. Centers of diversity and centers of origin. In: Frankel OH, Bonnet E. eds. Genetic Resources in Plants: Th eir Exploration and Conservation. Blackwell; 1970: pp. 33-42.
  • Semagn K. Genetic relationships among ten endod types as revealed by a combination of morphological, RAPD and AFLP markers. Hereditas 137: 149-156, 2002.
  • Tsombalova J, Haljak M, Ingver A et al. Genetic diversity of Estonian-grown spring wheat varieties assessed by microsatellite and morphological analyses. Soil and Plant Sci 58: 97-104, 2008.
  • McLauchlan A, Henry RJ, Isaac PG et al. Microsatellite analysis in cultivated hexaploid wheat and wild wheat relatives. Henry RJ. ed. Plant Genotyping: Th e DNA Fingerprinting of Plants, CABI Publishing; 2001: pp. 147-159.
  • Mahmood A, Baenziger PS, Budak H et al. Th e use of microsatellite markers for the detection of genetic similarity among winter bread wheat lines for chromosome 3A. Th eor Appl Genet 109: 1494-1503, 2004.
  • Zeb B, Khan IA, Ali A et al. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. African J Biotechnol 8: 4016-4019, 2009.
  • Mangini G, Taranto F, Giove SL et al. Identifi cation of durum wheat cultivars by a minimum number of microsatellite markers. Cereal Res Commun 38: 155-162, 2010.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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