Shamima Akhtar SHARMIN, Mohammad Jahangir ALAM, Md. Mominul İslam SHEIKH, Rashed ZAMAN, Muhammad KHALEKUZZAMAN, Sanjoy Chandra MONDAL, Mohammad Anwarul HAQUE, Mohammad Firoz ALAM, İftekhar ALAM

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine (BAP) or 6-(a,a-dimethylallylamino)-purine (2iP), were used either singly or in combination with naphthalene acetic acid (NAA) for shoot induction and multiplication, a single use of BAP was the most effective. As a carbon source, 3% (w/v) sucrose exhibits the greatest promotive effect on shoot initiation and proliferation compared with other carbon sources used. Among the basal media, full strength Murashige and Skoog (MS) media produced the best results, compared to other media studied. By using the most effective treatment from each category, an average of 13.2 shoots/per explant were produced after 6 weeks of culture. Moreover, 85% survival was achieved when rooted explants acclimatized ex vitro using a mixture of sterile sand, soil, and farmyard manure (1:1:1). In addition, antimicrobial activities of rhizome extracts were evaluated. Petroleum ether and chloroform extracts of field-grown rhizome showed potential antimicrobial properties against several human pathogenic bacteria including Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella sonnei, and Shigella dysenteriae, with a minimum inhibitory concentration (MIC) ranging from 0.03 to 0.5 mg/mL. Thus, the optimized micropropagation protocol may offer large-scale production of plantlets to meet industrial demand for the rhizome. Moreover, our results suggest the rhizome extract of C. aromatica is a promising antimicrobial agent.

Anahtar Kelimeler:

Key words: Antimicrobial activity, Curcuma aromatica, endangered, in vitro culture

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Keywords:

Key words: Antimicrobial activity, Curcuma aromatica, endangered, in vitro culture,

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Adelberg J (2010). Sucrose, water and nutrient use during stage II multiplication of two turmeric clones (Curcuma longa L.) in liquid medium. Sci Hortic 124: 262–267.
Alam I, Sharmin S, Naher MK, Alam MJ, Anisuzzaman M, Alam M (2013). Elimination and detection of viruses in meristemderived plantlets of sweet potato as a low-cost option toward commercialization. 3 Biotech 3: 153–164.
Arslan S, Silici S, Perçin D, Koç AN, Er Ö (2012). Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turk J Biol 36: 65–73.
Behura S, Sahoo S, Srivastava VK (2002). Major constituents in leaf essential oils of Curcuma longa L. and Curcuma aromatica Salisb. Curr Sci 83: 1311–1313.
Cenkci S, Kargioglu M, Dayan S, Konuk M (2007). Endangered status and propagation of an endemic plant species, Thermopsis turcica (Fabaceae). Asian J Plant Sci 6: 288–293.
Cheng JH, Wu WY, Liu WS, Chang G, Liu YL, Yang ZG, Li LN, Zhou H (1999). Treatment of 17 cases of patients with primary liver cancer with Curcuma aromatica oil infused via hepatic artery. Shijie Huaren Xiaohua Zazhi 7: 92.
Ditengou FA, Teale WD, Kochersperger P, Flittner KA, Kneuper I, van der Graaff E, Nziengui H, Pinosa F, Li X, Nitschke R et al. (2008). Mechanical induction of lateral root initiation in Arabidopsis thaliana. Proc Natl Acad Sci USA 105: 18818– 18823.
Dulak J (2005). Nutraceuticals as anti-angiogenic agents: hopes and reality. J Physiol Pharmacol 56: 51–69.
Erel ŞB, Reznicek G, Şenol SG, Yavaşoğulu NÜK, Konyalıoğlu S, Zeybek AU (2012). Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia. Turk J Biol 36: 75–84.
Karakaş FP, Yıldırım A, Türker A (2012) Biological screening of various medicinal plant extracts for antibacterial and antitumor activities. Turk J Biol 36: 641–652.
Kato A, Tohoyama H, Joho M, Inouhe M (2007). Different effects of galactose and mannose on cell proliferation and intracellular soluble sugar levels in Vigna angularis suspension cultures. J Plant Res 120: 713–719.
Kim JH, Shim JS, Lee SK, Kim KW, Rha SY, Chung HC, Kwon HJ (2002). Microarray-based analysis of anti angiogenic activity of demethoxycurcumin on human umbilical vein endothelial cells: crucial involvement of the down-regulation of matrix metalloproteinase. Jpn J Cancer Res 93: 1378–85.
Kumar V, Sikarwar RLS (2002). Observations on some rare and endangered plants of Chhattisgarh state, India. Phytotaxonomy 2: 135–142.
Loc NH, Doan TD, Kwon TH, Yang MS (2005). Micropropagation of zedoary (Curcuma zedoaria Roscoe)—a valuable medicinal plant. Plant Cell Tiss Org Cult 81: 119–122.
Mello MO, Carlos, Dias CTS, Amaral AFC, Melo M (2001). Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources. Sci Agric 58: 481–485.
Miachir JI, Romani VLM, Amaral AFC, Mello MO, Crocomo OJ, Melo M (2004). Micropropagation and callogenesis of Curcuma zedoaria Roscoe. Sci Agric (Piracicaba, Braz.) 61: 427–432.
Miachir JI, Romani VLM, Amaral AFC, Mello MO, Crocomo OJ, Melo M (2004). In vitro plant regeneration and genotype conservation of eight wild species of Curcuma. Biol Plant 48: 129–132.
Mohanty S, Panda MK, Subudhi E, Nayak S (2008). Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis. Biol Plantarum 52: 783–786.
Mujumdar AM, Naik DG, Dandge CN, Puntambekar HM (2000). Anti-inflammatory activity of Curcuma amada in albino rats. Indian J Pharmacol 32: 375–377.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
NCCLS (National Committee for Clinical Laboratory Standards) (1998). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Conidium-Forming Filamentous Fungi: Proposed Standard M38–P. NCCLS, Wayne, PA, USA.
Nadgauda RS, Mascarenhas AF, Hendre RR, Jagannathan V (1978). Rapid multiplication of turmeric (Curcuma longa Linn.) plants by tissue culture. Indian J Exp Biol 16: 120–122.
Nambiar MC, Pillai PKT, Sarma YN (1982). Seedling propagation in turmeric (Curcuma aromatica Salisb.). J Plantation Crops 10: 81–85.
Nayak S (2000). In vitro multiplication and microrhizome induction in Curcuma aromatica Salisb. Plant Growth Regul 32: 41–47.
Negi PS, Jayaprakasha GK, Jagan MRL, Sakariah KK (1999). Antibacterial activity of turmeric oil: a byproduct from curcumin manufacture. J Agric Food Chem 47: 4297–4300.
Orhan İE, Özçelik B, Kartal M, Kan Y (2012). Antimicrobial and antiviral effects of essential oils from selected Umbelliferae and Labiatae plants and individual essential oil components. Turk J Biol 36: 239–246.
Pitasawat B, Choochote W, Tuetun B, Tippawangkosol P, Kanjanapothi1 D, Jitpakdi A, Riyong D (2003). Repellency of aromatic turmeric Curcuma aromatica under laboratory and field conditions. J Vector Ecol 28: 234–240.
Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003). High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Rep 21: 1054– 1059.
Preethi TP, Shinija K, Rakhi KP, Sabu M, Madhusoodanan PV, Benjamin S (2010). Micropropagation and chemical profiling of Curcuma aromatica. J Trop Med Plants 11: 65–69.
Riek JD, Piqueras A, Debergh PC (1997). Sucrose uptake and metabolism in a double layer system for micropropagation of Rosa multiflora. Plant Cell Tiss Org Cult 47: 269–278.
Ringman JM, Frautschy SA, Cole GM, Masterman DL, Cummings JL (2005). A potential role of the curry spice curcumin in Alzheimer’s disease. Curr Alzheimer Res 2: 131–6.
Roy S (2004). Raychaudhuri SS. In vitro regeneration and estimation of curcumin content in four species of curcuma. Plant Biotech 21: 299–302.
Salvi N, George L, Eapen S (2000). Direct regeneration of shoots from immature inflorescence cultures of turmeric. Plant Cell Tiss Org Cult 62: 235–238.
Salvi N, George L, Eapen S (2001). Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss Org Cult 66: 113–119.
Salvi N, George L, Eapen S (2002). Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell Tiss Org Cult 68: 143–151.
Sharma S, Mukesh CS, Dharm VK (2010). Formulation and antimicrobial activity of 95% ethanolic-benzene-chloroform extract of Curcuma aromatica Salisb. Ann Biol Res 1: 153–156.
Sharma TR, Singh BM (1997). High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. Plant Cell Rep 17: 68– 72.
Shatnawi M, Anfoka G, Shibli R, Al-Mazra’awi M, Shahrour W, Arebiat A (2011). Clonal propagation and cryogenic storage of virus-free grapevine (Vitis vinifera L.) via meristem culture. Turk J Agric For 35: 173–184.
Shatnawi MA (2013). Multiplication and cryopreservation of yarrow (Achillea millefolium L., Asteraceae). J Agr Sci Tech 15: 163– 173.
Shatnawi MA, Frehat N, Makhadmeh I, Shibli R, Abu Ein A (2007). Influence of sugar source on growth and sugar uptakes of in vitro grown wild pear (Pyrus syriaca). Adv Hortic Sci 3: 133– 140.
Shatnawi MA, Krystyna AJ, Fraser RT (2004). In vitro propagation and cryostorage of Syzygium francissi (Myrtaceae) by the encapsulation-dehydration method. In Vitro Cell Dev BiolPlant 40: 403–407.
Shirgurkar MV, Naik VB, von Arnold S, Nadgauda RS, Clapham D (2006). An efficient protocol for genetic transformation and shoot regeneration of turmeric (Curcuma longa L.) via particle bombardment. Plant Cell Rep 25: 112–116.
Shirin F, Kumar S, Mishra Y (2000). In vitro plantlet production system for Kaempferia galanga, a rare Indian medicinal herb. Plant Cell Tiss Org Cult 63: 193–197.
Swedlund B, Locy RD (1993). Sorbitol as the primary carbon source for the growth of embryogenic callus of maize. Plant Physiol 103: 1339–1346.
Tefera W, Wannakrairoj S (2004). A micropropagation method for Korarima (Aframomum corrorima (Braun) Jansen. Sci Asia 30: 1–7.
Thomas TD, Shankar S (2009). Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight and Arnott, a rare medicinal plant. Plant Biotechnol Rep 3: 67–74.
Wilson B, Abrahamb G, Manjua VS, Mathew M, Vimala B, Sundaresan S, Nambisan B (2005). Antimicrobial activity of Curcuma zedoaria and Curcuma malabarica tubers. J Ethnopharmacol 99: 147–151.
Wuthi-udomlert M, Grisanapan W, Luanratana O, Caichompoo W (2000). Antifungal activity of Curcuma longa grown in Thailand. Southeast Asian J Trop Med Public Health 31: 178– 82.
Yoshioka T, Fujii E, Endo M, Wada K, Tokunaga Y, Shiba N, Hohsho H, Shibuya H, Muraki T (1998). Anti-inflammatory potency of dehydrocurdione, a zedoary-derived sesquiterpene. Inflamm Res 47: 476–481.
Zayova E, Stancheva I, Geneva M, Petrova M, Dimitrova L (2013). Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turk J Biol 37: 106–113.
Zhou XJ, Gan XS, Wang LX, Qian JM, Li CS, Meng PL (1997). Inhibition of proliferation and induction of apoptosis of elemene on Himeg cell line. Zhonghua Xueyexue Zazhi 18: 263–264.