Interaction between Glomus geosporum, Azotobacter chroococcum, and Bacillus coagulans and their Influence on Growth and Nutrition of Melia azedarach L.
A green house nursery study was conducted to assess the interaction between arbuscular mycorrhizal fungus, a nitrogen fixing bacterium, Glomus geosporum, Azotobacter chroococcum, and a mycorrhiza helper bacterium (MHB), Bacillus coagulans, in soil and their consequent effect on growth and nutrition of Melia azedarach L. seedlings. Triple inoculation of G. geosporum, A. chroococcum, and B. coagulans resulted in maximum plant biomass, N, P, Zn, and Cu uptake, and biovolume and quality index of M. azedarach seedlings. It also increased the mycorrhizal root colonization and spore numbers in the root zone soil of the inoculated plants over uninoculated control plants. The enzyme activity, namely acid phosphatase and dehydrogenase, in the root zone soil was found high in the 3-combination treatments and low in the uninoculated control. Triple inoculation with G. geosporum + A. chroococcum + B. coagulans proved to be the best microbial consortium for inoculating M. azedarach at nursery level in order to get healthy and vigorously growing seedlings.
Interaction between Glomus geosporum, Azotobacter chroococcum, and Bacillus coagulans and their Influence on Growth and Nutrition of Melia azedarach L.
A green house nursery study was conducted to assess the interaction between arbuscular mycorrhizal fungus, a nitrogen fixing bacterium, Glomus geosporum, Azotobacter chroococcum, and a mycorrhiza helper bacterium (MHB), Bacillus coagulans, in soil and their consequent effect on growth and nutrition of Melia azedarach L. seedlings. Triple inoculation of G. geosporum, A. chroococcum, and B. coagulans resulted in maximum plant biomass, N, P, Zn, and Cu uptake, and biovolume and quality index of M. azedarach seedlings. It also increased the mycorrhizal root colonization and spore numbers in the root zone soil of the inoculated plants over uninoculated control plants. The enzyme activity, namely acid phosphatase and dehydrogenase, in the root zone soil was found high in the 3-combination treatments and low in the uninoculated control. Triple inoculation with G. geosporum + A. chroococcum + B. coagulans proved to be the best microbial consortium for inoculating M. azedarach at nursery level in order to get healthy and vigorously growing seedlings.
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- Tabatabai MA. Chemical and Microbiological properties. In: Page AL, Miller RH, Kennye DR. ed. Methods of Soil Analysis. Part 2. American society of Agronomy, Madison, Wisconsin; pp 903-947. 1982.
- Gerdemann JW, Nicolson TH. Spores of mycorrhizal Endogone species extracted form soil by wet sieving and decanting. Trans Br Mycol Soc 46: 235-244, 1963.
- Phillips JH, Hayman DS. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158- 161, 1970.
- Giovannetti M, Mosse B. An evaluation of techniques to measure vesicular-arbuscular infection in roots. New Phytol 84: 489-500, 1980.
- Manjunath A, Bagyaraj DJ, Gowda HSG et al. Dual inoculation with VA mycorrhiza and Rhizobium is beneficial to Leucaena. Plant Soil 78: 445-448, 1984.
- Poi SC, Ghosh G, Kabi MC et al. Response of chickpea to combined inoculation with Rhizobium, phosphobacteria and mycorrhizal organisms. Zentralbl Microbiol 144: 249-253, 1989.
- Barea JM, Bonis AF, Olivares J et al. Interaction between Azospirillum and VA mycorrhiza and their effects on growth and nutrition of maize and ryegrass. Soil Biol Biochem 15: 706-709, 1983.
- Veeraswamy T, Padmavathy T, Venkateshwarlu K et al. Interaction effects of Glomus intraradices and Azospirillum lipoferum on sorghum. Ind J Microbio 32: 305-308, 1992.
- Barea JM, Azcon R, Azcon-Aquilar C et al. Vesicular-arbuscular fungi in nitrogen fixing systems. Methods Microbiol 24: 391-416, 1992.
- Meyer JR, Linderman RG. Response of subterranean clover to dual inoculation with vesicular arbuscular mycorrhizal fungi and a plant growth promoting bacterium, Pseudomonas putida. Soil Biol Biochem 18: 185-190, 1986.
- Umamaheshwar N. Studies on the diversity of am fungi from rhizosphere soils and the roots of some fiber yielding crops and their influence on growth and productivity, PhD, Bharathidasan University, Tichy, Tamilnadu, India; 2007.
- Bagyaraj DJ, Menge JS. Interaction between a VA mycorrhizal fungi and Azotobacter and their effects on rhizosphere microflora and plant growth. New Phytol 80: 567-573, 1978. Duponnois R, Garbaye J. Effect of dual inoculation of Douglus fir with the ectomycorrhizal fungus Laccaria laccata and mycorriza helper bacteria (MHB) in two bare root forest nurseries. Plant Soil 138: 169-176, 1991.
- Sekar I, Vanangamudi K, Suresh KK et al. Effect of Bio-fertilizers on the seedling biomass, VAM colonization enzyme activity and phosphorus uptake in the Shola tree species. My Forest 31: 21- 26, 1995
- Lakshmipathy R, Chandrika K, Balakrishna Gowda et al. Response of Saraca asoca (Roxb.) de Wilde to inoculation with Glomus mosseae, Bacillus coagulans and Trichoderma harzianum. J Soil Biol Ecol 21 (1&2): 76-80. 2001.