Milena KEREMIDARSKA MARKOVA, Kamelia HRISTOVA PANUSHEVA, Natalia KRASTEVA, Ekaterina RADEVA, Dimitar MITEV, Brett PAULL, Pavel NESTERENKO, Joseph SEPITKA, Ita JUNKAR, Ales IGLIC

Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of maturebone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particlesin different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This methodallows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties,including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effecton adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized onPPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the sampleswere negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCsin comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support thehypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymalstem cells.

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