Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells
In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature
bone tissue (0.2-2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAD-
MSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles
in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method
allows variation only in the reduced elastic modulus (Er') with increase in the particle concentration, while the other surface properties,
including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect
on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on
PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples
were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs
in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the
hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal
stem cells.
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