Expression of a bacterial <i>aroA</i> gene confers tolerance to glyphosate in tobacco plants
Glyphosate is a widely used herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-encoding
aroA
gene in the shikimate pathway. The discovery and cloning of
the
aroA
gene with high resistance is central to breeding a transgenic
glyphosate-resistant plant. A novel
aroAPantoea
gene from
Pantoea
G-1 was previously isolated and cloned. The aroAPantoea
enzyme was
defined as a new class I EPSPS with glyphosate resistance. The
aroAPantoea
gene was introduced into tobacco through
Agrobacterium-mediated transformation. The transgenic tobacco plants were confirmed by PCR, RT-PCR, and Southern blot. The analysis of glyphosate
resistance also showed that the transgenic tobacco plants could survive at 15 mM glyphosate; the glyphosate resistance level of the
transgenic plants is higher than the agricultural application level recommended by most manufacturers. Overall, this study shows that
aroA
Pantoea
can be used as a candidate gene for the development of genetically modified crops.
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