Polyphenolic contents of <i>Teucrium polium</i> L. and <i>Teucrium scordium</i> L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes
Teucrium
species have been used in traditional medicine for treatment of different diseases. The aim of this study was to
investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts
of
Teucrium polium
and
Teucrium scordium
using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood
lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caffeic,
chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated
with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts
significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of
T. scordium
, except the
lowest, and all concentrations of
T. polium
extracts in combined treatment with MMC significantly reduced the frequency of MN.
The extract of
T.
polium
did not significantly affect the nuclear division index (NDI), whereas
T. scordium
in higher concentrations,
separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in
combination with MMC have antimutagenic (
T.
polium)
and
proapoptotic effects
(
T. scordium
), which indicates their protective effects
in PBLs.
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