Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

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Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Sine ÖZMEN TOĞAY, Ayhan TEMİZ, Ayten ÇELEBİ, Leyla AÇIK, Sıddika Songül YALÇIN

Development of durable antimicrobial surfaces containing silver- and zinc-ion-exchanged zeolites

Zeynep Ustaoğlu İYİGÜNDOĞDU, Selami DEMİRCİ, Nurcan BAÇ, Fikrettin ŞAHİN

Expression and purification of porcine Akirin2 in Escherichia coli

Xiaoling CHEN, Zhiqing HUANG, Bo ZHOU, Huan WANG, Gang JIA, Jiayun QIAO

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Handan BALTA, Özge KARAKAŞ METİN, Funda ŞENTÜRK AKFIRAT, Fahriye ERTUĞRUL, Semra HASANÇEBİ

Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity

Liaqat HUSSAIN, Javaria IKRAM, Kanwal REHMAN, Muhammad TARIQ, Muhammad IBRAHIM, Muhammad Sajid Hamid AKASH

Evaluation of genotoxicity and cytotoxicity induced by different molecular weights of polyethylenimine/DNA nanoparticles

Leila GHOLAMI, Hamid Reza SADEGHNIA, Majid DARROUDI, Reza Kazemi OSKUEE

Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

Tayfun KAYA, Belma ASLIM, Ergin KARİPTAŞ

Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture

Marija PETRIC, Sladana JEVREMOVIC, Milana TRIFUNOVIC, Vojin TADIC, Snezana MILOSEVIC, Angelina SUBOTIC

A preliminary analysis of the gene all0012 of Anabaena PCC 7120

Shen TIAN, Si-Li CHEN, Feng-Jiao GONG

Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk

Zeliha YILDIRIM, Yaselin İLK, Metin YILDIRIM, Kader TOKATLI, Nilgün ÖNCÜL