Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture

We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L-1 thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in order to examine the influence of the above-mentioned pretreatments and nutrient medium composition on enzyme activity. Enzyme activity was measured 7, 14, 21, and 28 days after the start of morphogenesis induction. Superoxide dismutase and catalase showed the highest activity at the beginning of morphogenesis in vitro. Peroxidase activity was the highest in bulb segments immediately after isolation following pretreatments and on both nutrition media. We also determined the effect of pretreatment coupled with low temperature (4 °C) on growth and development of F. meleagris in vitro and chlorophyll and carotenoids content.

Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture

We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L-1 thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in order to examine the influence of the above-mentioned pretreatments and nutrient medium composition on enzyme activity. Enzyme activity was measured 7, 14, 21, and 28 days after the start of morphogenesis induction. Superoxide dismutase and catalase showed the highest activity at the beginning of morphogenesis in vitro. Peroxidase activity was the highest in bulb segments immediately after isolation following pretreatments and on both nutrition media. We also determined the effect of pretreatment coupled with low temperature (4 °C) on growth and development of F. meleagris in vitro and chlorophyll and carotenoids content.

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  • Aebi H (1984). Catalase in vitro. Method Enzymol 105: 121–126.
  • Alscher RG, Erturk N, Heath LS (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53: 1331–1341.
  • Apel K, Hirt H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 55: 373–399.
  • Auh CK, Scandalios JG (1997). Spatial and temporal responses of the maize catalases to low temperature. Physiol Plantarum 101: 149–156.
  • Bagnoli F, Capuana M, Racchi M (1998). Developmental changes of catalase and superoxide dismutase isoenzymes in zygotic and somatic embryos of horse chestnut. Aust J Plant Physiol 25: 909–913.
  • Balen B, Krsnik-Rasol M, Simeon-Rudolf M (2003). Isoenzymes of peroxidase and esterase related to morphogenesis in Mammillaria gracillis Pfeiff. tissue culture. J Plant Physiol 160: 1401–1406.
  • Bannister JV, Bannister WH, Rotilio G (1987). Aspects of the structure, function and application of superoxide dismutase. Crit Rev Biochem 22: 111–118.
  • Beyer WF, Fridowich I (1987). Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161: 559–566.
  • Bowler C, Van Montagu M, Inzé D (1992). Superoxide dismutase and stress tolerance. Ann Rev Phys Chem 43: 83–116.
  • Bowler C, Van Camp W, Van Montagu M, Inzé D (1994). Superoxide dismutases in plants. Crit Rev Plant Sci 13: 199–218.
  • Bradford MM (1976). A rapid sensitive method for the quantification of microgram quantities of proteins using the principle of protein-dye binding. Anal Biochem 72: 248–254.
  • Cassells AC, Curry RF (2001). Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org 64: 145–157.
  • Çelik Ö, Atak Ç (2012). The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turk J Biol 36: 339–356.
  • Correa-Aragunde N, Graziano M, Chevalier C, Lamattina L (2006). Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato. J Exp Bot 57: 581–588.
  • Davletova S, Meszaros T, Miskolczi P, Oberschall A, Torok K, Magyar Z, Dudits D, Daek M (2001). Auxin and heat shock activation of a novel member of calmodulin like domain protein kinase gene family alfalfa cells. J Exp Bot 52: 215–221.
  • Dudits D, Gyorgyey J, Bogre L, Bako L (1995). Molecular biology of somatic embryogenesis. In: Thorpe TA, editor. In Vitro Embryogenesis in Plants. Dordecht: Kluwer Academics Publishers, pp. 267–308.
  • Edge R, McCarvey DJ, Truscott TJ (1997). The carotenoids as antioxidants: a review. J Photoch Photobio 41: 189–200.
  • Ganesan M, Jayabalan N (2004). Evaluation of hemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR 2). Plant Cell Rep 23: 181–187.
  • Gilamore AM (1997). Mechanistic aspects of xantifille cycledependent photoprotection of higher plant chloroplasts and leaves. Physiol Plantarum 99: 197–209.
  • Grossmann K (2000). Mode of action of auxinic herbicides: a new ending to a long, drawn out story. Trends Plant Sci 5: 506–508.
  • Huyste VRB, Carnis WL (1982). Progress and prospects in the use of peroxidase to study cell development. Photochemistry 21: 1843–1847.
  • Inzé D, Van Montagu M (1995). Oxidative stress in plants. Curr Opin Biotech 6: 153–158.
  • Jia SR, Kumar PP, Kush A (1996). Oxidative stress in Agrobacteriuminduced tumors on Kalanchoe plants. Plant J 10: 545–551.
  • Joersbo M, Andersen JM, Okkels FT, Rajagopal R (1989). Isoperoxidases as markers of somatic embryogenesis in carrot suspension cultures. Physiol Plantarum 76: 10–16.
  • Kairong C, Ji L, Gengmei X, Jianlong L, Lihong W, Yafu W (2002). Effect of hydrogen peroxide on synthesis of proteins during somatic embryogenesis in Lycium barbarum. Plant Cell Tiss Org 68: 187–193.
  • Karami O, Aghavaisi B, Pour AM (2009). Molecular aspects of somatic-to-embryogenic transition in plants. J Chem Biol 2: 177–190.
  • Karami O, Saidi A (2010). The molecular basis for stress-induced acquisition of somatic embryogenesis. Mol Biol Rep 37: 2493– 250
  • Kim JH, Baek MH, Chung BY, Wi SG, Kim JS (2004). Alternations in the photosynthesis pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gammairradiated seeds. J Plant Biotech 47: 314–321.
  • Krsnik-Rasol M, Jelaska S, Šerman D (1982). Isoperoxidases—early indicators of somatic embryoid differentiation in pumpkin tissue. Acta Bot Croat 41: 33–39.
  • Kukavica B, Veljović-Jovanović S (2004). Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing. Physiol Plantarum 122: 321–327.
  • Kukulczanka K, Kromer K, Czastka B (1989). Propagation of Fritillaria meleagris L. through tissue culture. Acta Hortic 251: 147–153.
  • Kovtun Y, Chiu WL, Tena G, Sheen J (2000). Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade. P Natl Acad Sci USA 97: 2940–2945.
  • Lambe P, Mutambel HSN, Fouche JG, Deltour R, Foidart JM, Gaspar T (1997). DNA methylation of organogenesis totipotency and plant neoplastic progression? In Vitro Cell Dev Pl 33: 155–162.
  • Langens-Gerrits MM, Miller WBM, Croes AF, Klerk GJ (2003a). Effect of low temperature on dormancy breaking and growth after planting in lily bulblets regarded in vitro. Plant Growth Regul 40: 267–275.
  • Langens-Gerrits MM, Klerk GJ, Croes A (2003b). Phase change in lily bulblets regenerated in vitro. Physiol Plantarum 119: 590– 5
  • Lichtenhaler HK (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148: 350– 3
  • Miller WBM, Langhans RW (1990). Low temperatures alters carbohydrate metabolism in Easter lily bulbs. Hort Sci 25: 463–465.
  • Mizuno M, Kamei M, Tsuchida H (1998). Ascorbate peroxidase and catalase cooperate for protection against hydrogen peroxide generated in potato tubers during low-temperature storage. Biochem Mol Biol Int 44: 717–726.
  • Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–497.
  • Nikolić M, Mišić D, Maksimović V, Jevremović S, Trifunović M, Subotić A (2008). Effect of low temperature on rooting rate and carbohydrate content of Fritillaria meleagris bulbs formed in culture in vitro. Arch Biol Sci 60: 5–6.
  • Olmos E, Piqueras A, Martinez-Solano JR, Hellin E (1997). The subcellular localization of peroxidase and implication of oxidative stress in hyperhydrated leaves of regenerated carnation plants. Plant Sci 130: 97–105.
  • Paek KY, Murthy HN (2002). High frequency of bulblet regeneration from bulb scale section of Fritillaria thunbergii. Plant Cell Tiss Org 68: 247–252.
  • Paek KY, Sung NS, Park CH (1996). Several factors affecting bulblet regeneration from the culture of scale segment and node-bud in fritillary as medicinal bulbous plant. Acta Hortic 440: 499– 50
  • Passardi F, Cosio C, Penel C, Dunand C (2005). Peroxidases have more function than a Swiss army knife. Plant Cell Rep 24: 255–265.
  • Passardi F, Penel C, Dunand C (2004). Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9: 534–540.
  • Pasternak TP, Prinsen E, Ayaydin F, Miskolczi P, Potters G, Asard H, Van Onckelen HA, Dudits D, Fehér A (2002). The role of auxin, pH, and stress in the activation of embryogenic cell division in leaf protoplast-derived cells of alfalfa. Plant Physiol 129: 1807–1819.
  • Petrić M, Subotić A, Jevremović S, Trifunović M (2011). Somatic embryogenesis and bulblet regeneration in snakehead fritillary (Fritillaria meleagris L.). Afr J Biotechnol 10: 16181–16188.
  • Petrić M, Subotić A, Trifunović M, Jevremović S (2012). Morphogenesis in vitro of Fritillaria spp. In: Tuyl J, Arens P, editors. Floriculture and Ornamental Biotechnology 6, Bulbous Ornamentals (Special Issue). London, UK: Global Science Books, pp. 78–89.
  • Sangwan RS, Bourgeois Y, Brown S, Vasseur G, Sangwan-Norreel B (1992). Characterization of competent cells and early events of Agrobacterium mediated genetic transformation in Arabidopsis thaliana. Planta 188: 439–456.
  • Sharifi G, Ebrahimzadeh H (2010). Changes of antioxidant enzyme activity and isoenzyme profiles during in vitro shoot formation in saffron (Crocus sativus L.). Acta Biol Hung 61: 73–89.
  • Sharma P, Jha AB, Dubey RS, Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012: 1–
  • Subotić A, Trifunović M, Jevremović S, Petrić M (2010). Morphohistological study of direct somatic embryogenesis in endangered species Fritillaria meleagris. Biol Plantarum 54: 592–596.
  • Sun CS, Wang DY (1991). Fritillaria spp. (Fritillary): in vitro culture and the regeneration of plants. In: Bajaj YPS, editor. Biotechnology in Agriculture and Forestry: Medicinal and Aromatic Plants III, 1 ed., Vol. XV. Berlin, Germany: Springer, pp. 258–269.
  • Türktaş M, Aslay M, Kaya E, Ertuğrul F (2012). Molecular characterization of phylogenetic relationships in Fritillaria species inferred from chloroplast trnL-trnF sequences. Turk J Biol 36: 552–560.
  • Veljović-Jovanović S, Kukavica B, Stevanović B, Navari-Izzo F (2006). Senescence and drought related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. J Exp Bot 57: 1769–1778.
  • Vranová E, Van Breusegem F, Dat J, Belles-Boix E, Inzé D (2002). The role of active oxygen species in plant signal transduction. In: Scheel D, Wasternack C, editors. Plant Signal Transduction: Frontiers in Molecular Biology. Oxford, UK: Oxford University Press, pp. 45–73.
  • Walck JL, Cofer MS, Hiddayati SN (2010). Understanding the germination of bulbils from an ecological perspective: a case study on Chinese yam (Dioscorea polystachya). Ann BotLondon 106: 945–95.
  • Witomska M, Lukaszewska A (1997). Bulblet regeneration in vitro from different explants of Fritillaria imperialis. Acta Hortic 430: 331–338.
  • Yahraus T, Chandra S, Legendre L, Low PS (1995). Evidence for a mechanically induced oxidative burst. Plant Physiol 109: 1259– 12
  • Zayova E, Stancheva I, Geneva M, Petrova M, Dimitrova L (2013). Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turk J Biol 37: 106–113.
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  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
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