Dilek AKYIL, Sedat OKTAY, Recep LİMAN, Yasin EREN, Muhsin KONUK

Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia

Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia (At-ext) were investigated using both Allium cepa root meristematic cells and a short-term mutation assay in Salmonella typhimurium with TA98 and TA100 strains in the presence or absence of S9 mix, respectively. In the Allium tests, EC50 value was determined as 50 g/L, and 0.5 × EC50, EC50, and 2 × EC50 concentrations of At-ext were applied to onion tuber roots. Methyl methane sulfonate (MMS, 10 ppm) and distilled water were used as positive and negative controls, respectively. The mitotic index (MI) increased in all concentrations in comparison to control at each exposure time except 25 and 50 g/L at 12 h. While stickiness, chromosome laggards, bridges, and disturbed anaphase-telophase were observed in anaphase-telophase cells, pro-metaphase, c-metaphase, polyploidy, and binuclear cells were observed in other cells. It was determined that At-ext have no mutagenic effect on S. typhimurium TA98 and TA100 strains in the presence or absence of S9 mix. The results were statistically analyzed in SPSS using Duncan's multiple range test. These results indicate that At-ext has genotoxic activity in A. cepa root meristematic cells.

Anahtar Kelimeler:

Key words: Allium, Ames, chromosome aberration, mutagenicity

Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia

Keywords:

Key words: Allium, Ames, chromosome aberration, mutagenicity,

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Huber-Morath A. Achillea L. In: Davis PH. ed. Flora of Turkey and the East Aegean Islands. Edinburgh University Press; 1975, vol 5: pp. 224-252.
Cheers G. Botanica: the Illustrated A-Z of over 10,000 Garden Plants and How to Cultivate Th em. Könemann Publications. Hong Kong; 1999.
Duman Achillea L. In: Güner A, Özhatay N, Ekim TKH, Başer H. C. eds. Flora of Turkey and the East Aegean Islands. Edinburgh University Press; 2000 11: pp. 158-159.
Yeşilada E, Honda G, Sezik E et al. Traditional medicine in Turkey IV. Folk medicine in the Mediterranean subdivision. J Ethnopharmacol 39: 31-38, 1993.
Fujita T, Sezik E, Tabata M et al. Traditional medicine in Turkey VII. Folk medicine in middle and west Black Sea regions. Econ Bot 49: 406-422, 1995.
Honda G, Yeşilada E, Tabata M et al. Traditional medicine in Turkey VI. Folk medicine in west Anatolia: Afyon, Kütahya, Denizli, Muğla, Aydin Provinces. J Ethnopharmacol 53: 75-87, 1996.
Baytop T. Türkiye’de Bitkiler ile Tedavi, Phytoterapy in Turkey. Second ed. Nobel Tıp Kitabevleri Ltd. Şti. İstanbul; 1999. 8. Schulz V, Hänsel R, Tyler V. Rational Phytotherapy: A Physicianıs Guide to Herbal Medicine. Springer. Berlin; 2001.
Boswell-Ruys CL, Ritchie HE, Brown-Woodman PD. Preliminary screening study of reproductive outcomes aft er exposure to yarrow in the pregnant rat. Birth Defects Res B Dev Reprod Toxicol 68: 416-420, 2003.
Kargıoğlu M, Cenkci S, Serteser A et al. An ethnobotanical survey of inner-west Anatolia, Turkey. Hum Ecol 36: 763-777, 2008.
Kargıoğlu M, Cenkci S, Serteser A et al. Traditional uses of wild plants in the middle Aegean region of Turkey. Hum Ecol 38: 429- 450, 2010.
Davies MG, Kersey PJ. Contact allergy to yarrow and dandelion. Contact Dermatitis 14: 256-257, 1986.
Hausbn BM, Bheuer J, Weglewski J et al. Α-peroxyachifolid and other new sensitizing sesquiterpene lactones from yarrow (Achillea millefolium L., Compositae). Contact Dermatitis 24: 274, 1991.
Compes E, Bartolomé B, Fernández-Nieto M et al. Occupational asthma from dried fl owers of Carthamus tinctorius (saffl ower) and A. millefolium (yarrow). Allergy 61: 1239-1240, 2006.
Fiskesjö G. Th e Allium test as standard in environmental monitoring. Hereditas 102: 99-112, 1985.
Grant WF. Cytogenetics studies of agricultural chemicals in plants. In: Genetic Toxicology an Agricultural Perspective. Plenum Press. New York; 1992: pp. 335-378.
Rank J. Th e method of Allium anaphase-telophase chromosome aberration assay. Ekologija (Vilnius) 1: 38-42, 2003.
Saxena PN, Chauhan LKS, Gupta SK. Cytogenetic eff ects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: spectroscopic basis of chromosome damage. Toxicology 216: 244-252, 2005.
Konuk M, Liman R, Ciğerci İH. Determination of genotoxic eff ect of boron on Allium cepa root meristematic cells. Pakistan J Bot 39: 73-79, 2007.
Liman R, Ciğerci İH, Akyıl D et al. Determination of genotoxicity of fenaminosulf by Allium and comet tests. Pestic Biochem Phys 99: 61-64, 2011.
Fiskesjö G. Th e Allium test—an alternative in environmental studies: the relative toxicity of metal ions. Mutat Res 197: 243- 260, 1988.
Konuk M, Akyıl D, Liman R et al. Examination of the mutagenic eff ects of some pesticides. Fresen Environ Bull 17: 439-442, 2008.
Uysal A, Durak Y, Aladag MO. Investigation of mutagenic eff ects of some plant growth regulators on Salmonella/microsome test system. Fresen Environ Bull 19: 2170-2175, 2010.
Liman R, Akyıl D, Eren Y et al. Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test. Chemosphere 80: 1056-1061, 2010.
Maron DH, Ames BN. Revised methods for the Samonella typhimurium mutagenicity test. Mutat Res 113: 173-215, 1983.
Mortelmans K, Zeiger E. Th e Ames Salmonella/microsome mutagenicity assay. Mutat Res 445: 29-60, 2000.
Ames B, McCann J, Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat Res 31: 347, 1975.
Sofowora A. Th e state of medicinal plants research in Nigeria. In: Proceeding of workshop held at Ile-Ife. Nigeria; 1999: pp.1-373.
Yıldız M, Ciğerci İH, Konuk M et al. Determination of genotoxic eff ects of copper sulphate and cobalt chloride in Allium cepa root cells by chromosome aberration and comet assays. Chemosphere 75: 934-938, 2009.
Dean BJ, Brooks TM, Hodson-Walkerand G et al. Genetic toxicology testing of 41 industrial chemicals. Mutat Res 153: 57- 77, 1985.
Websterand PL, Macleod RD. Th e root apical meristem and its margin. In: Waishel Y, Eshel A, Kafk afi U. eds. Plant Roots: Th e Hidden Half (second ed.). Marcel Dekker; 1996: pp. 51-76.
Fusconi A, Repetto O, Bona E et al. Eff ect of cadmium on meristem activity and nucleus ploidy in roots of Pisum sativum L. Cv. frisson seedlings. Environ Exp Bot 58: 253-260, 2006.
Fernandes TCC, Mazzeoand DEC, Marin-Morales MA. Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifl uralin herbicide. Pestic Biochem Phys 88: 252-259, 2007.
Hoshina MM. Evaluation of a possible contamination of the waters of the Claro River-Municipality of Rio Claro, part of the Corumbataí River Basin, with mutagenicity tests using Allium cepa. State University of São Paulo, Rio Claro, Sp. (in Portuguese), 2002.
Evseeva TI, Stanislav A, Geras’kinand I et al. Genotoxicity and toxicity assay of water sampled from a radium production industry storage cell territory by means of Allium-test. J Environ Radioact 68: 235-248, 2003.
Scolnicand D, Halazonetis T. Chfr defi nes a mitotic stress checkpoint that delays entry into metaphase. Nature 406: 430- 435, 2000.
Ajayand KL, Sarbhoy RK. Cytogenetic studies on the eff ect of some chlorinated pesticides. Cytologia 53: 427-436, 1988.
Luo LZ, Werner KM, Gollinand SM et al. Cigarette smoke induces anaphase bridges and genomic imbalances in normal cells. Mutat Res 554: 375-385, 2004.
Kumari M, Mukherjee A, Chandrasekaran N. Genotoxicity of silver nanoparticles in Allium cepa. Sci Total Environ 407: 5243- 5246, 2009.
Ateeq B, Farah MA, Ali MN et al. Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. Mutat Res 514: 105-113, 2002.
Graf U, Alonso Moraga A, Castro R et al. Genotoxicity testing of diff erent types of beverages in the Drosophila wing somatic mutation and recombination test. Food Chem Toxicol 32: 423- 430, 1994.
De Sant’anna JR, Franco CC, Miyamoto CT et al. Genotoxicity of Achillea millefolium essential oil in diploid cells of Aspergillus nidulans. Phytother Res 23(2): 231-235, 2009.
Montanari T, Ernesto DE, Carvalho J et al. Antispermatogenic eff ect of Achillea millefolium L. in mice. Contraception 58: 309- 313, 1998.
Dalsenter PR, Cavalcanti AM, Androde AJM et al. Reproductive evaluation of aqueous crude extract of Achillea millefolium L. (Asteraceae) in Wistar rats. Reprod Toxicol 18: 819-823, 2004.
Takzare N, Hosseini MJ, Hamideh Mortazavi S et al. Th e eff ect of Achillea millefolium extract on spermatogenesis of male Wistar rats. Hum Exp Toxicol 1-7, 2010.
Trifunovic S, Vajs V, Juranic Z et al. Cytotoxic constituents of Achillea clavennae from Montenegro. Phytochemistry 67: 887- 893, 2006.
Ghantous A, Nasse N, Saab I et al. Saliba structure–activity relationship of seco-tanapartholides isolated from Achillea falcata for inhibition of hacat cell growth. Eur J Med Chem 44: 3794-3797, 2009.
Teixeira RO, Camparoto ML, Mantovani MS et al. Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in vitro and in vivo assays. Genet Mol Biol 26: 551-555, 2003.
Roncada T, Vicentiniand VEP, Mantovani MS. Possible modulating actions of plant extracts on the chromosome breaking activity of Mmc and Ara C in human lymphocytes in vitro. Toxicol In Vitro 18: 617-622, 2004.