Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis

Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M1V2 and M1V3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants were genetically 27.5% different from the control plants. The greatest difference encountered between the control and mutants was 47%, which was detected in mutant plants produced by 20 or 30 Gy gamma irradiation and regenerated in selection medium containing 100 mM NaCl.

Anahtar Kelimeler:

Key words: Gamma radiation, in vitro mutagenesis, mutation breeding, potato, RAPD-PCR, salt stress

Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis

Keywords:

Key words: Gamma radiation, in vitro mutagenesis, mutation breeding, potato, RAPD-PCR, salt stress,

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1. Pareek A, Singla-Pareek S, Sopory SK et al. Analysis of salt stress-related transcriptome fi ngerprints from diverse plant species. In: Varshney RK, Tuberosa R. eds. Genomics-Assisted Crop Improvement: Vol. 1: Genomics Approaches and Platforms. Springer; 2007: pp. 267-287.
2. Blumwald E, Grover A. Salt tolerance. In: Halford NG. ed. Plant Biotechnology: Current and Future Uses of Genetically Modifi ed Crops. John Wiley & Sons Ltd.; 2006: pp. 206-22.
3. Leblebici Z, Aksoy A, Duman F. Infl uence of salinity on the growth and heavy metal accumulation capacity of Spirodela polyrrhiza (Lemnaceae). Turk J Biol 35: 215-220, 2011.
4. Munns R, Richards RA. Recent advances in breeding wheat for drought and salt stresses. In: Jenks MA et al. eds. Advances in Molecular Breeding toward Drought and Salt Tolerant Crops. Springer; 2007: pp. 565-585.
5. Mba C, Afza R, Jain SM et al. Induced mutations for enhancing salinity tolerance in rice. In: Jenks MA et al. eds. Advances in Molecular Breeding toward Drought and Salt Tolerant Crops. Springer; 2007: pp. 413-454.
6. Müft üoğlu NM, Dardeniz A, Sungur A et al. Bazı sofralık üzüm çeşitlerinin tuza toleranslarının belirlenmesi. S Ü Ziraat Fakültesi Dergisi 20: 37-42, 2006.
7. Sen A, Alikamanoglu S. Eff ects of salt stress on growth parameters and antioxidant enzymes of diff erent wheat (Triticum aestivum L.) varieties on in vitro tissue culture. FEB 20-2a: 489-495, 2011.
8. Sairam RK, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci 86: 407-421, 2004.
9. Saleem MY, Mukhtar Z, Cheema AA et al. Induced mutation and in vitro techniques as a method to induce salt tolerance in Basmati rice (Oryza sativa L.). Int J Environ Sci Tech 2/2: 141-145, 2005.
10. Das A, Gosal SS, Sidhu JS et al. Induction of mutations for heat tolerance in potato by using in vitro culture and radiation. Euphytica 114: 205-209, 2000.
11. Lu G, Zhang X, Zou Y et al. Eff ect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers. Plant Cell Tiss Org 88: 319-327, 2007.
12. Szarejko I, Forster BP. Doubled haploidy and induced mutation. Euphytica 158: 359-370, 2007.
13. El-Sayed OE, Rizkalla AA, Sabri SRS. In vitro mutagenesis for genetic improvement of salinity tolerance in wheat. Res J Agr Biol Sci 4: 377-383, 2007.
14. Hewawasam WDCJ, Bandara DC, Aberathne WM. New phenotypes of Crossandra infundibuliformis var. Danica through in vitro culture and induced mutations. Tropical Agricul Res 16: 253-270, 2004.
15. Lee IS, Kim DS, Lee SJ et al. Selection and characterizations of radiation-induced salinity-tolerant lines in rice. Breeding Sci 53: 313-318.
16. Saif-ur-Rasheed M, Asad S, Zafar Y et al. Use of radiation and in vitro techniques for development of salt tolerant mutants in sugarcane and potato. In Vitro Techniques for Selection of Radiation Induced Mutations Adapted to Adverse Environmental Conditions. IAEA Vienna, IAEATECDOC-1227, ISSN 1011-4289, 2001.
17. Ahloowalia BS, Maluszynski M. Induced mutations – a new paradigm in plant breeding. Euphytica 118: 167-173, 2001. 18. Alikamanoğlu, S. Effi ciency of the gamma radiation in the induction of in vitro somatic mutations. J Cell Molec Biol 1: 19-24, 2002.
19. FAO/IAEA: Database of mutant variety and genetic stocks. Available from: URL: www.mvd.iaea.org [accessed 10 March 2009].
20. Gulsen O, Uzun A, Pala H et al. Development of seedless and Mal Secco tolerant mutant lemons through budwood irradiation. Sci Hortic 112: 184-190, 2007
21. Zhen HR. In vitro technique for selection of radiation induced mutants of sweet potato. In Vitro Techniques for Selection of Radiation Induced Mutations Adapted to Adverse Environmental Conditions. IAEA Vienna, IAEA TECDOC 1227, ISSN 1011-4289, 2001.
22. Byun M, Kwon H, Park S. Recent advances in genetic engineering of potato crops for drought and saline stress tolerance. In: Jenks MA et al. eds. Advances in Molecular Breeding toward Drought and Salt Tolerant Crops. Springer; 2007: pp. 713-737.
23. Nhut DT, Nguhen NH, Th uy DTT. A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production. Sci Hortic 110: 230-234, 2006.
24. Manrique LM. Potato Production in the Tropics. Manrique International Agrotech. Honolulu; 2000.
25. Sharabash MT. Radiation induced variation in potato for tolerance to salinity using tissue culture technique. In Vitro Techniques for Selection of Radiation Induced Mutations Adapted to Adverse Environmental Conditions. IAEA Vienna, IAEA-TECDOC-1227 ISSN 1011-4289, 2001.
26. Murashige T, Skoog F. A revised medium for rapid growth and bioassays tobacco cultures. Physiol Plant 15: 473-497, 1962.
27. Gosal SS, Das A, Gopal J et al. In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato. In Vitro Techniques for Selection of Radiation Induced Mutations Adapted to Adverse Environmental Conditions. IAEA Vienna, IAEA-TECDOC-1227 ISSN 1011-4289, 2001.
28. Zar JH. Biostatistical Analysis. Prentice-Hall, Inc. Englewood Cliff s, New Jersey; 1984.
29. Abbas SJ, Rasool G, Shah SRU et al. Analysis of genetic diversity in Pakistani potato cultivars by using randomly amplifi ed polymorphic DNA (RAPD) primers. Am Eurasian J Sustain Agric 2: 50-53, 2008.
30. Atak C, Alikamanoğlu S, Açık L et al. Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD. Mutat Res 556: 35-44, 2004.
31. Babaoğlu S, Açık L, Çelebi A et al. Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-PAGE methods. Gazi University J Sci 17: 25-33, 2004.
32. Wolf K, Rijini J. Rapid detection of genomic variability in Chrysanthemum using random primers. Heredity 71: 335-341, 1993.
33. Toker C, Yadav SS, Solanki IS. Mutation breeding. In: Yadav SS, McNeil DL, Stevenson PC. eds. Lentil: An Ancient Crop for Modern Times. Springer; 2007: pp. 209-224.
34. Predieri S, Di Virgilio N. In vitro mutagenesis and mutant multiplication. In: Jain SM, Häggman H. eds. Protocols for Micropropagation of Woody Trees and Fruits. Springer; 2007: 323-333.
35. Donini P, Sanino A. Induced mutation in plant breeding: current status and future outlook. In: Mohan Lain S, Brar DS, Ahloowalia BS. eds. Somaclonal Variation and Induced Mutations in Crop Improvement. Kluwer Academic Publishers; 1998: pp. 255-291.
36. Lüleyap H. Moleküler Genetiğin Esasları. Nobel Kitabevi. Adana; ISBN: 978-605-397-005-7, 2008.
37. Kumar G, Kumar Rai P. Genetic repairing through storage of gamma irradiated seeds in inbred maize (Zea mays L.). Turk J Biol 33: 195-204, 2009.