Belgin Göçmen TAŞKIN, Nilden VARDARELİ, Ersin DOĞAÇ, Ramazan MAMMADOV, Vatan TAŞKIN

Genetic diversity of natural Cyclamen alpinum populations

The focus of this study was Cyclamen alpinum (formerly C. trochopteranthum). Habitat fragmentation, environmental degradation, and overharvesting of tubers have exerted pressure on native populations of this valuable ornamental species. Although the entire Cyclamen genus is in the Convention on International Trade in Endangered Species of Wild Fauna and Flora-Appendix II (CITES II), no species has yet been red-listed. Estimating the level and distribution of genetic variation in populations of rare and endemic species is important for conserving genetic diversity within a species in the context of well-developed conservation strategies. Currently, DNA markers are the most effective means used to infer genetic variation at the molecular level in conservation genetics. In this study, random amplified polymorphic DNA (RAPD) analysis was employed to assess the genetic diversity within and among 6 natural C. alpinum populations in the south and southwest of Turkey. A total of 190 loci were determined by using 15 polymorphic primers. Total genetic variation (HT) was 0.27 ± 0.02. A high proportion of this variation, 0.16 ± 0.01 (59.26%), was due to within-population genetic variation (HS). The genetic differentiation coefficient (GST) was 0.41, and the level of gene flow (Nm) within a generation among the 6 populations studied was 0.73. As a result of these findings, we propose in situ combined with ex situ conservation of all C. alpinum populations. In addition, our results support prior recommendations to add C. alpinum to the International Union for Conservation of Nature and Natural Resources (IUCN) Red List under the critically endangered (CR) category.

Anahtar Kelimeler:

Key words: Cyclamen alpinum, Cyclamen trochopteranthum, genetic diversity, RAPD

Genetic diversity of natural Cyclamen alpinum populations

Keywords:

Key words: Cyclamen alpinum, Cyclamen trochopteranthum, genetic diversity, RAPD,

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Mathew B, Özhatay N. Türkiye’nin Siklamenleri, Türkiye’de Doğal Olarak Yetişen Siklamen Türlerinin Tanıtım Rehberi. Doğal Hayatı Koruma Derneği Yayınları. İstanbul; 2001 (in Turkish).
Weising K, Nybom H, Wolff K et al. DNA Fingerprinting in Plants: Principle, Methods, and Applications. CRC Press. Boca Raton, FL, USA; 2005.
Winter P, Kahl G. Molecular marker technologies for plant improvement. World J Microb Biotechnol 11: 438-448, 1995.
Khanuja SPS, Shasany AK, Daroker MP et al. DNA fi ngerprinting of plant genetic resources: the need of time. J Med Arom Pl Sci 20: 348-351, 1998.
Fritsch P, Rieseberg LH. Th e use of random amplifi ed polymorphic DNA (RAPD) in conservation genetics. In: Smith B, Wayne KR. eds. Molecular Genetic Approaches in Conservation. Oxford University Press; 1996: pp. 54-73.
Şahin O, Bürün B. Cyclamen alpinum (hort. Dammann ex Sprenger, 1892)’un morfolojisi, ekolojisi ve sitolojisi. SDÜ Fen Dergisi 5: 5-15, 2010.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15, 1987.
Cullings KW. Design and testing of a plant-specifi c PCR primer for ecological and evolutionary studies. Mol Ecol 1: 233-240, 1992.
Sharma R, Mahla HR, Mohapatra T et al. Isolating plant genomic DNA without liquid nitrogen. Plant Mol Biol Rep 21: 43-50, 2003.
Williams JGK, Kubelic AR, Livak KJ et al. DNA polymorphisms amplifi ed by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535, 1990.
Kimura M, Crow JF. Th e number of alleles that can be maintained in a fi nite population. Genetics 49: 725-38, 1964.
Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70: 3321-3323, 1973.
Lewontin RC. Th e apportionment of human diversity. Evol Biol 6: 381-398, 1972.
Yeh FC, Boyle T, Rongcai Y et al. POPGENE Version 1.32: Microsoft Windows-Based Freeware for Population Genetic Analysis. University of Alberta. Edmonton, Alberta, Canada; 1999.
Nei M. Genetic distance between populations. Am Nat 106: 283-292, 1972.
Saitou N, Nei M. Th e neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406-425, 1987.
Lewis PO, Zaykin D. Genetic Data Analysis: Computer Program for the Analysis of Allelic Data (version 1.1). Free program distributed by the authors over the Internet; 2001.
Reid WV, Money HA, Cropper A et al. Ecosystems and Human Well-Being: Synthesis/Millennium Ecosystem Assessment. Island Press. Washington, DC; 2005.
Ciotir C, Yesson C, Culham A. Future predictions of Cyclamen distribution in the Mediterranean region. Nature Precedings, doi:10.1038/npre.2009.3066.1, 2009.
Yesson C, Culham A. A phyloclimatic study of Cyclamen. BMC Evol Biol 6: 72, 2006.
Ellstrand NC, Elam DR. Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol Evol 24: 217-242, 1993.
Godt MJW, Johnson BR, Hamrick JL. Genetic diversity and population size in four rare southern Appalachian plant species. Conserv Biol 10: 796-805, 1996.
Godt MJW, Hamrick JL. Genetic diversity in rare southeastern plants. Nat Areas J 21: 61-70, 2001.
Newman D, Pilson D. Increased probability of extinction due to decreased genetic eff ective population size: experimental populations of Clarkia pulchella. Evolution 51: 354-362, 1997.
Fischer M, Matteis D. Eff ects of population size on performance in the rare plant Gentianella germanica. J Ecol 86: 195-204, 1998.
Hamrick JL, Godt MJ. Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL et al. eds. Plant Population Genetics, Breeding, and Genetic Resources. Sinauer; 1989: pp. 43-63.
Falk DA, Holsinger KE. Genetics and Conservation of Rare Plants. Oxford University Press. New York; 1991.
Aff re L, Th ompson JD, Debussche M. Genetic structure of continental and island populations of the Mediterranean endemic Cyclamen balearicum (Primulaceae). Amer J Bot 84: 437-451, 1997.
Hamrick JL, Godt MJW. Eff ects of life history traits on genetic diversity in plant species. Philos Trans R Soc Lond B Biol Sci 351: 1291-1298, 1996.
Cruse-Sanders JM, Hamrick JL. Genetic diversity in harvested and protected populations of wild American ginseng, Panax quinquoefolius L. (Araliaceae). Am J Bot 91: 540-548, 2004.
Jordano P, Godoy JA. RAPD variation and population genetic structure in Prunus mahaleb (Rosaceae), an animal-dispersed tree. Mol Ecol 9: 1293-1305, 2000.
Goto S, Tsuda Y, Koike Y et al. Eff ects of landscape and demographic history on genetic variation in Picea glehnii at the regional scale. Ecol Res 24: 1267-1277, 2009.
Th iel-Egenter C, Gugerli F, Alvarez N et al. Eff ects of species traits on the genetic diversity of high-mountain plants: a multi- species study across the Alps and the Carpathians. Global Ecol Biogeogr 18: 78-87, 2009.
Chen SY, Ma X, Zhang XQ et al. Genetic variation and geographical divergence in Elymus nutans Griseb. (Poaceae: Triticeae) from West China. Biochem Syst Ecol 37: 716-722, 2009.
Zhang JH, McDonald MB, Sweeney PM. Testing for genetic purity in petunia and cyclamen seed using random amplifi ed polymorphic DNA markers. HortScience 32: 246-247, 1997.
Laura M, De Benedetti L, Bruna S et al. Cyclamen persicum Mill.: Somatic embryogenesis and RAPD analysis of embryogenic callus. Acta Hort 625: 137-144, 2003.
Naderi R, Alaey M, Khalighi A et al. Inter- and intra-specifi c genetic diversity among cyclamen accessions investigated by RAPD markers. Sci Hortic 122: 658-661, 2009.