Li ZHANG, Hong-xia ZHAO, Xing FAN, Meng WANG, Chun-bang DING, Rui-wu YANG

Genetic diversity among Salvia miltiorrhiza Bunge and related species inferred from nrDNA ITS sequences

To investigate the genetic diversity and phylogenetic relationships of Salvia miltiorrhiza and related species, we analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) region for 7 accessions of Salvia miltiorrhiza and another 23 samples from other taxa within the genus Salvia by maximum parsimony and Bayesian inference analyses. There were 257 variation sites amounting to 40.8% of the total base pairs. All of the data revealed abundant genetic diversity in the genus Salvia. The results showed that the tested materials could be grouped into 3 clusters, and the 3 different occasions in Salvia were clustered into 1 clade, which suggests that the species from different occasions form independent lineages. We can easily distinguish S. miltiorrhiza from a variety of Salvia species in this manner. Moreover, according to cluster analysis, S. bowleyana, S. yunnanensis, and S. cavaleriei var. simplelefolia are good or potential germplasm resources for S. miltiorrhiza.

Anahtar Kelimeler:

Key words: ITS, genetic diversity, Salvia miltiorrhiza

Genetic diversity among Salvia miltiorrhiza Bunge and related species inferred from nrDNA ITS sequences

Keywords:

Key words: ITS, genetic diversity, Salvia miltiorrhiza,

PDF

___

Standley P, Williams L. Labiatae. Fieldiana Bot 24: 237-317, 1973. 2. Wu CY, Li XW. Flora of China. Science Press. Beijing; 1990.
Aşkun T, Başer KHC, Tümen G et al. Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turk J Biol 34: 89-95, 2010. 4. Pharmacopoeia Commission of the People’s Republic of China. Pharmacopoeia of the People’s Republic of China. Chemical Industry Press. Beijing; 2002.
Soltis DE, Soltis PS, Tate JA. Advances in the study of polyploidy since plant speciation. New Phytol 161: 173-191, 2003.
Baldwin BG, Sanderson MJ, Porter JM et al. Th e ITS region of nuclear ribosomal DNA: a valuable source of evidence in angiosperm phylogeny. Ann Missouri Bot Gard 82: 247-277, 1995.
Hsiao C, Chatterton NJ, Asay KH et al. Phylogenetic relationships of the monogenomic species of the wheat tribe, Triticeae (Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences. Genome 38: 211-223, 1995.
Zhang W, Qu LJ, Gu H et al. Studies on the origin and evolution of tetraploid wheat based on the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Th eor Appl Genet 104: 1099-1106, 2002.
Steane DA, Scotland RW, Mabberley DJ et al. Molecular systematics of Clerodendrum (Lamiaceae): ITS sequences and total evidence. Am J Bot 86: 98-107, 1999.
Prather LA, Monfi ls AK, Posto AL et al. Monophyly and phylogeny of Monarda (Lamiaceae): evidence from the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. Syst Bot 27: 127-137, 2002.
Bräuchler CB, Meimberg H, Heubl G. Molecular phylogeny of Menthinae (Lamiaceae, Nepetoideae, Mentheae) - taxonomy, biogeography, and confl icts. Mol Phylogenet Evol 55: 501-523, 2010.
Dirmenci T, Dündar E, Deniz G et al. Morphological, karyological, and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle. Turk J Bot 34: 159-170, 2010.
Howard C, Bremner PD, Fowler MR et al. Molecular identifi cation of Hypericum perforatum by PCR amplifi cation of the ITS and 5.8S rDNA region. Planta Med 75: 864-869, 2009.
Walker JB, Sytsma KJ, Treutlein J et al. Salvia (Lamiaceae) is not monophyletic: implications for the systematic, radiation, and ecological specializations of Salvia and tribe Mentheae. Am J Bot 91: 1115-1125, 2004.
Walker JB, Sytsma KJ. Staminal evolution in the genus Salvia (Lamiaceae): molecular phylogenetic evidence for multiple origins of the staminal lever. Ann Bot 100: 375-391, 2007.
Wang H, Wang Q. Analysis of rDNA ITS sequences of Radix et Rhizoma Salviae Miltiorrhizae and plants of Salvia L. Chin Trad Herbal Drugs 36: 1381-1385, 2005.
Wang Y, Li DH, Zhang YT. Analysis of ITS sequences of some medicinal plants and their related species in Salvia L. Acta Pharmacol Sin 42: 1301-1313, 2007.
Xu H, Wang ZT, Cheng KT et al. Comparison of rDNA ITS sequences and tanshinones between Salvia miltiorrhiza populations and Salvia species. Bot Stud 50: 127-135, 2009.
Doyle JT, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15, 1987.
Liu QL, Ge S, Tang HB et al. Phylogenetic relationships in Elymus (Poaceae: Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. New Phytol 170: 411-420, 2006.
Th ompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting positions-specifi c gap penalties and weight matrix choice. Nucl Acids Res 22: 4673- 4680, 1994.
Tamura K, Dudley J, Nei M et al. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) soft ware version 4.0. Mol Biol Evol 24: 1596-1599, 2007.
Swoff ord DL. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4.0b10. Sinauer Associates. Sunderland, Massachusetts; 2003.
Huelsenbeck JP, Ronquist FR. MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754-755, 2001.
Posada D, Crandall KA. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818, 1998.
Alziar G. Catalogue synonymique des Salvia L. du monde (Lamiaceae) I-VI. Biocosme Mesogéen 5(3-4): 87-136; 6(1-2, 4): 79-115, 163-204; 7(1-2): 59-109; 9(2-3): 413-497; 10(3-4): 33-117, 1988-1993.
Epling C. A revision of Salvia, subgenus Calosphace. Repert Spec Nov Regni Veg Beih 110: 1-383, 1939.
Steward AN. Manual of Vascular Plants of the Lower Yangtze Valley, China. Oregon State College. Corvallis, Oregon; 1958.
Chen CC, Chen HT, Chen YP et al. Taiwan Yao Hsueh Tsa Chih 38: 226-230, 1986.
Li B, Niu FD, Lin ZW et al. Diterpenoids from the roots of Salvia przewalskii. Phytochemistry 30: 3815-3817, 1991.
Xue M, Shi YB, Cui Y. Chemical constituents from Salvia przewalskii Maxim. Nat Prod Res Dev 12: 27-32, 2000.
Chen WS, Jia XM, Zhang WD. Chemical constituents in the roots of Salvia przewalskii Maxim. Acta Pharmacol Sin 38: 354- 357, 2003.
Zhao JB. Research and evaluation of utilization on S. przewalskii. J Chin Med 26: 528-530, 2003.