İlham POYRAZ ERÖZ, Emel SÖZEN, Ebru ATAŞLAR, İsmail POYRAZ

Determination of genetic relationships among Velezia L. (Caryophyllaceae) species using RAPD markers

Bu çalışmada, RAPD (Random Amplified Polymorphic DNA) belirteçleri Türkiye’de yetişen Velezia L. türlerinin genetik akrabalık seviyelerini belirlemede kullanılmıştır. 14 RAPD primerinden, toplam 432 bant elde edilmiştir. RAPD belirteçlerindeki polimorfizm oranı yüksek (% 98,6) ve her türü ayırt etmek için yeterlidir. Paylaşılan ortak bant seviyesi türler arasındaki genetik benzerliği değerlendirmek ve UPGMA (unweighted pair group method with arithmetic mean) tabanlı bir dendrogram oluşturmak için kullanılmıştır. Türler arasındaki genetik akrabalıkların, morfolojik karakterlerin kullanımıyla elde edilen verilerle tamamen uyumlu olduğu bulunmuştur. Elde edilen sonuçlar RAPD tekniğinin Velezia türleri arasındaki genetik akrabalıkların belirlenmesinde olduğu kadar türlerin tanımlanmasında da başarıyla kullanılabileceğini göstermiştir. Ayrıca bu teknik gelecekteki çalışmalarda, özellikle tehlike altındaki Velezia türlerinin korunması için ön verileri sağlamada etkin olarak kullanılabilir.

RAPD markörleri kullanılarak Velezia L. (Caryophyllaceae) türlerinin genetik akrabalıklarının belirlenmesi

Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high (98.61%) and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identification. Furthermore, it can be efficiently employed in future studies to provide preliminary data for conservation of endangered Velezia species.

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