Bioactive compounds from discarded mushroom beds

Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 µg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were grown on direct mushroom bed extract, 0.2% glucose-containing mushroom bed extract, and common production medium for determination of growth and lignolytic activities. Since lignolytic activities were high with the direct mushroom bed extract, this medium was further diluted to 1:1 and 1:2 ratios and again tested for growth and lignolytic activities with F. feei, T. gibbosa, and T. elegans. It was determined that the 1:1 ratio gave good results for all these organisms. The button mushroom bed extract was concentrated using a rotary evaporator and compared for growth and lignolytic enzymes with dried powder extract using F. feei, T. gibbosa, and T. elegans. Growth was high with these 3 organisms in the dried powder extract, but lignin peroxidase activity was the highest with the rotary evaporator extract using T. gibbosa. The results indicate that these waste mushroom bed extracts can be used as cost-effective media for the growth of microorganisms and for the production of bioactive compounds and industrial enzymes.

Bioactive compounds from discarded mushroom beds

Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 µg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were grown on direct mushroom bed extract, 0.2% glucose-containing mushroom bed extract, and common production medium for determination of growth and lignolytic activities. Since lignolytic activities were high with the direct mushroom bed extract, this medium was further diluted to 1:1 and 1:2 ratios and again tested for growth and lignolytic activities with F. feei, T. gibbosa, and T. elegans. It was determined that the 1:1 ratio gave good results for all these organisms. The button mushroom bed extract was concentrated using a rotary evaporator and compared for growth and lignolytic enzymes with dried powder extract using F. feei, T. gibbosa, and T. elegans. Growth was high with these 3 organisms in the dried powder extract, but lignin peroxidase activity was the highest with the rotary evaporator extract using T. gibbosa. The results indicate that these waste mushroom bed extracts can be used as cost-effective media for the growth of microorganisms and for the production of bioactive compounds and industrial enzymes.

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  • Mizuno TS. Bioactive biomolecules of mushrooms: food function and medicinal eff ect of mushroom fungi. Food Rev Int 11: 151-166, 1995. 2. Beelman RB, Royse DJ, Chikthimmah N. Bioactive components in button mushroom Agaricus bisporus (J.Lge) Imbach (Agaricomycetideae) of nutritional, medicinal and biological importance (review). Int J Med Mush 5: 321-337, 2003.
  • Enman J, Rova U, Berglund KA. Quantifi cation of the bioactive compound Eritadenine in selected strains of shiitake mushroom (Lentinus edodes). J Agric Food Chem 55: 1177- 1180, 2007.
  • Klaus AS, Kozarski MS, Niksia MP. Infl uence of bioactive compounds extracted from mushroom Ganoderma lucidum on b and t cells. Proc Nat Sci Matica Srpska Novi Sad 116: 217- 223, 2009.
  • Akamatsu Y. Reutilization of culture wastes of Pleurotus ostreatus and Pholiota nameko for cultivation of Lyophyllum decastes. J Wood Sci 44: 417-420, 1998.
  • Siddhant, Singh CS. Recycling of spent oyster mushroom substrate to recover additional value. Kathmandu Univ J Sci Eng Technol 5: 66-71, 2009.
  • Dubois MK, Gilles JK, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356, 1956. 8. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428, 1959.
  • Sadasivam S, Manikam A. Biochemical Methods for Agricultural Sciences. Wiley Eastern Ltd. New Delhi; 1992.
  • Lowry OH, Rosebrough NJ, Farr AL et al. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275, 1951.
  • Coll MP, Fernandez-Abalos JM, Villanueva JR et al. Purifi cation and characterization of a phenoloxidase (laccase) from the lignin-degrading basidiomycete PM1 (CECT 2971). Appl Environ Microbiol 59: 2607-2613, 1993.
  • Sarakanen S, Razal RA, Piccariello T et al. Lignin peroxidase: toward a classifi cation of its role in vivo. J Biol Chem 266: 3636- 3643, 1999.
  • Parzeznski A, Hynn VB, Crawford RL. Comparison of lignin I and peroxidase M2 from the white rot fungus Phanerochete chrysosporium. Arch Biochem Biophys 244: 750-765, 1986.
  • Cardenas F, Alvarez E, de Castro-Alvarez MS et al. Screening and catalytic activity in organic synthesis of novel fungal and yeast lipase. J Mol Cataly B Enz 14: 111-123, 2001.
  • Shukla P, Gupta K. Ecological screening for lipolytic molds and process optimization for lipase production from Rhizopus oryzae KG-5. J App Sci Environ Sanit 2: 35-42, 2007.
  • Mandels M, Andreotti R, Roche C. Measurement of saccharifying cellulase. Biotechnol Bioeng Symp 6: 1-23, 1976.
  • Youssef GA, Botros WA, Daba AS. Screening for enzymatic and biological activity of Pleurotus ostreatus - a popular edible mushroom in Egypt. Mushroom News 2008. Available at http://www.highbeam.com/doc/1G1-176049780.html.
  • Keay L, Wildi BS. Proteases of the genus Bacillus. I. Neutral proteases. Biotechnol Bioeng 12: 179-212, 1970.
  • Paranthaman R, Alagusundaram K, Indhumathi J. Production of protease from rice mill wastes by Aspergillus niger in solid state fermentation. World J Agri Sci 5: 308-312, 2009.
  • Beri V, Gupta R. Acetylcholinesterase inhibitors neostigmine and physostigmine inhibit induction of alpha-amylase activity during seed germination in barley, Hordeum vulgare var. Jyoti. Life Sci 80: 2386-2388, 2007.
  • Jones RL, Varner JE. Th e bioassay of gibberellins. Planta 72: 155-161, 1967.
  • Sinegani AS, Emtiazi G. Th e relative eff ects of some elements on the DNS method in cellulase assay. J Appl Sci Environ Mgt 10: 93-96, 2006.
  • Biely P. Microbial xylanolytic systems. Trends Biotech 3: 286- 290, 1985.
  • Aebi H. Catalase in vitro. Methods Enzymol 105: 121-126, 1984.
  • Zadrazil F. Th e conversion of straw into feed by basidiomycetes. Eur J Appl Microbiol 4: 273-281, 1977.
  • Vaughan D, Malcolm RE, Ord BG. Infl uence of humic substances on biochemical processes in plants. In: Vaughan D, Malcolm RE. eds. Soil Organic Matter and Biological Activity. Kluwer Academic Publishers; 1985: pp. 77-100.
  • Shukry WM, El-Fallal AA, El-Bassiouny HMS. Eff ect of spent wheat straw on growth, growth hormones, metabolism and rhizosphere of Cucumis sativa. Egypt J Physiol Sci 23: 39-69, 1999.
  • Ranyanathan DS, Selvaseelan DA. Residual eff ect of mushroom spent rice straw compost on yield and nutrient uptake in green gram. Madras Agric J 81: 478, 1994.
  • López Castro RI, Delmastro S, Curvetto NR. Spent oyster mushroom substrate in a mix with organic soil for plant pot cultivation. Mycol Appl Int 20: 17-26, 2008.
  • Özgüven AI. Th e opportunities of using mushroom compost waste in strawberry growing. Turk J Agri For 22: 601-607, 1998.
  • Abak K, Yanmaz R, Koçyiğit AE. Mantar kompost atığının sebze yetiştiriciliğinde değerlendirilmesi. Tarım ve Köyişleri Bak Dergisi 25: 16-18, 1986.
  • Çelikel G. Organik ve Inorganik Kökenli Bazı Ortamların Serada Topraksız Yetiştiricilikte Kullanılabilirliği ile Domates, Biber ve Patlıcanda Bitki Gelişmesi, Verim, Erkencilik ve Kalite Üzerine Etkileri, PhD, Çukurova University Institute of Science and Technology, 1994.
  • Paksoy M. Domateste Topraksız Yetiştiricilikte Değişik Substrat Karışımlarının ve Bitki Kök Bölgesi Isıtmasının Bitki Gelişimi, Verim, Erkencilik ve Ürün Kalitesine Etkileri, PhD, Çukurova University Institute of Science and Technology, 1995.
  • Sharma AD, Jandaik CL. Studies on recycling of Pleurotus waste. Mushroom Newsletter for the Tropics 6: 13-15, 1985.
  • Nakaya M. Recycling of waste mushroom substrate for mushroom cultivation. I. Cultivation of Pleurotus cornucopiae using the sawdust from waste shiitake bed log. Mushroom Sci Biol 4: 9-13, 1997.
  • Shimoda T, Nishibori K,  Shirouchi T et al. Pretreatment of waste mushroom bed and method of converting the same to yield sugars and ethanol. US Patent No. 2008/0213850.
  • Lee JW, Koo BW, Choi JW et al. Evaluation of waste mushroom logs as a potential biomass resource for the production of bioethanol. Bioresource Technol 99: 2736-2741, 2008.
  • Tajbakhsh J, Abdoli MA, Goltapeh EM et al. Trend of physico- chemical properties change in recycling spent mushroom compost through vermicomposting by epigeic earthworms Eisenia foetida and E. andrei. J Agric Tech 4: 185-198, 2008.
  • Finney KN, Ryu C, Sharifi VN et al. Th e reuse of spen t mushroom compost and coal tailings for energy recovery: Comparison of thermal treatment technologies. Bioresource Technol 100: 310-315, 2009.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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