Cytotoxic action of lead nitrate on cytomorphology of Trigonella foenum-graecum L.

The present experiment was undertaken to determine the mutagenic potential of lead on the cytomorphology of Trigonella foenum-graecum L. and to determine the maximum concentration of lead nitrate that induces maximum genetic variability. The seeds were treated with 6 different concentrations (10, 25, 50, 100, 200, and 400 ppm) of lead nitrate. The higher concentrations of lead nitrate significantly reduced plant height, number of branches per plant, pollen fertility, and yield; higher concentrations also caused variation in the plants at the seedling and mature stages. In addition, concentrations affected the pairing of homologous chromosomes and spindle formation. This resulted in laggards, bridges, fragments, multipolar orientation of chromosomes, and reduced chiasmata frequency. On the basis of these results, it was concluded that lower concentrations of metal did not significantly affect the cytomorphology of Trigonella, while higher concentrations of lead nitrate were found to be more mutagenic and cytotoxic.

Cytotoxic action of lead nitrate on cytomorphology of Trigonella foenum-graecum L.

The present experiment was undertaken to determine the mutagenic potential of lead on the cytomorphology of Trigonella foenum-graecum L. and to determine the maximum concentration of lead nitrate that induces maximum genetic variability. The seeds were treated with 6 different concentrations (10, 25, 50, 100, 200, and 400 ppm) of lead nitrate. The higher concentrations of lead nitrate significantly reduced plant height, number of branches per plant, pollen fertility, and yield; higher concentrations also caused variation in the plants at the seedling and mature stages. In addition, concentrations affected the pairing of homologous chromosomes and spindle formation. This resulted in laggards, bridges, fragments, multipolar orientation of chromosomes, and reduced chiasmata frequency. On the basis of these results, it was concluded that lower concentrations of metal did not significantly affect the cytomorphology of Trigonella, while higher concentrations of lead nitrate were found to be more mutagenic and cytotoxic.

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  • Lewis TE, McIntosh AW. Covariation of selected trace elements with binding substrates in ores from two contaminated sediments. Bull Environ Contam Toxicol 43: 518-528, 1989. 2.. Brüning T, Chronz C, Th ier R et al. Occurrence of urinary tract tumors in miners highly exposed to dinitrotoluene. J Occup Environ Med 41: 144-149, 1999. 3.. Nishioka H. Mutagenic activity of metal compound in bacteria. Mutat Res 31: 185-189, 1975.
  • Singh OP, Sharma A. Eff ect of certain metallic pollution on plant genetic system. Nucleus 23: 15-29, 1980. 5. Singh OP, Sharma A. Eff ects of Mercury, Cadmium, and Lead on Plant Chromosomes. Prof. S. M. Sircar Memorial Volume. Today and Tomorrow Printers. New Delhi; 1982: pp. 305-312.
  • Sato M, Gaul H. Eff ect of ethyl methanesulfonate on the fertility of barley. Radiat Bot 7: 7-10, 1967.
  • Natarajan AT, Shivasankar G. Studies on modifi cation of mutations response of barley seeds to ethyl methanesulfonate. Mol Gen Genet 96: 13-21, 1965.
  • Krishna G, Shivashankar G, Nath J. Mutagenic response of rhodes grass (Chloris gayana Kunth.) to gamma rays. Environ Exp Bot 24: 197-205, 1984.
  • Anis M, Wani AA. Caff eine induced morphological variability in fenugreek (Trigonella foenum-graecum L.). Cytologia 62: 243-246, 1997.
  • Reddi TVVS. Cytogenetics of Mutants Induced by Physical and Chemical Mutagens in Th ree Varieties of Rice (Oryza sativa L.), PhD, Andhra University, Waltair, India, 1977.
  • Bhat TA, Khan AH, Parveen S et al. Studies on the eff ect of EMS on meiosis and pollen fertility in Vicia faba L. Adv Plant Sci 19: 243-247, 2006.
  • Dimitrova I. Studies of Grass Plant Under Conditions of Industrial Pollution with Heavy Metal and Sulphur Dioxide, author’s summary of PhD, Plovdiv University “P. Hilendarski”, 1993.
  • Dimitrova I, Ivanova E. Eff ect of heavy metal-soil pollution on some morphological and cytogenetical characteristics of fl ax (Linum usitatissum L.). J Balkan Ecol 6: 212-218, 2003.
  • Laxmi V, Gupta MN, Dutta SK. Investigation on an induced green seed coat mutant of Trigonella foenum-graecum L. Cytologia 40: 373-378, 1983.
  • Tickoo JK, Chandra N. Mutagen induced polygenic variability in mungbean (Vigna radiata Welezek L.). Indian J Genet 59: 193-201, 1999.
  • Singh NP, Singh M, Lal JP. Gamma rays and EMS induced genetic variability of quantitative traits in urd bean (Vigna mungo (L.) Hepper). Indian J Genet 60; 89-96, 2000.
  • Bhat TA, Khan AH, Parveen S. Eff ect of gamma rays on certain cytomorphological parameters in two varieties of Vicia faba L. Adv Plant Sci 19: 227-232, 2006.
  • El-Ghamery AA, El-Kholy MA, Abou El-Yousser MA. Evaluation of cytological eff ects of Zn2+ in relation to germination and root growth of Nigella sativa L. and Triticum aestivum L. Mutat Res 537: 29-41, 2003.
  • Grant WF, Owens ET. Chromosome aberration assays in Pisum for the study of environmental mutagens. Mutat Res 488: 93- 118, 2001.
  • Ahmad S. Meiotic studies in two cultivars of Cicer arietinum L. aft er gamma irradiation. Cytologia 58: 61-65, 1993.
  • Bhat TA, Khan AH, Parveen S. Comparative analysis of meiotic abnormalities induced by gamma rays, EMS and MMS in Vicia faba L. J Ind Bot Soc 84: 45-48, 2005.
  • Kumar G, Tripathi R. Anomalous nucleolar and chromosomal organization in induced phenodeviants of grass pea. Cytologia 79: 345-350, 2007.
  • Liu DH, Liu WS, Jiang W et al. Evaluation of metal ion toxicity on root tip cell by Allium test. Israel J Plant Sci 43: 125-133, 1995.
  • Ishido M, Kunimoto M. Regulation of cell fate by cadmium and zinc. J Health Sci 47: 9-13, 2000.
  • Evans HJ. Chromosome aberrations induced by ionising radiations. Int Rev Cytol 13: 221-231, 1962.
  • Jayabalan N, Rao GR. Gamma radiation induced cytological abnormalities in Lycopersicon esculentum Mull. var. Pusa Ruby. Cytologia 52: 1-4, 1987.
  • Kumar G, Singh V. Comparative analysis of meiotic abnormalities induced by gamma rays and EMS in barley. J Ind Bot Soc 82: 19-22, 2003.
  • Zeerak NA. Cytogenetical eff ect of gamma rays and ethylmethane sulphonate in brinjal (Solanum melongena L.). Cytologia 56: 639-643, 1992.
  • Gaulden ME. Hypothesis: some mutagens directly alter specifi c chromosomal proteins thus produce chromosome stickiness. Mutagenesis 2: 357-365, 1987.
  • Saylor LG, Smith BN. Meiotic irregularities in species of interspecifi c hybrids in Pisum. Am J Bot 53: 453-468, 1966.
  • Sinha SSN, Godward MBE. Radiation studies in Lens culinaris. Ind J Genet 32: 331-339, 1972.
  • Kumar G, Tripathi A. Mutagenic response of caff eine in Capsicum annum L. J Indian Bot Soc 83: 136-140, 2004.
  • Maria De Souza A, Pagliarini MS. Cytomixis in Brassica napus var. Oleifera and Brassica compestris var. Oleifera (Brassicaceae). Cytologia 62: 25-29, 1997.
  • Kaul KK. Cytomixis in pollen mother cells of Alopecuris arundinaceus Pois. Cytologia 55: 169-173, 1990.
  • Bhat TA, Parveen S, Khan AH. MMS-induced cytomixis in pollen mother cell of broad bean (Vicia faba L.). Turk J Bot 30: 273-279, 2006.
Turkish Journal of Biology-Cover
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  • Yayın Aralığı: Yılda 6 Sayı
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