Dilnawaz Syed AHMAD, Mubashar Syed SABİR, Halimi MOHAMMED, Yousaf SALİHUDDİN

Evolutionary relationship and divergence based on SDS-PAGE of Elaegnus umbellata (Thunb.) populations, a multipurpose plant from the Himalayas

Elaeagnus umbellata (Thunb.) Himalaya (Pakistan, Çin, Hindistan) bölgesinde ayrıca Kore ve Japonya da bulunan Elaeagnaceae familyasına ait sonbahar zeytini olarak isimlendirilen bir bitkidir. Azad Jammu ve Kashmir Pakistan bölgesinden sekiz tohum Tarım Teknoloji Labaratuvarlarına getirilen örneklerin toplam protein profilleri SDS-PAGE yöntemi ile mukayese edilmiştir. Sonuçlara gore sonbahar zeytini populasyonu kendine özgü protein band profile ile diğerlerinden ayrılmıştır. Jelde moleküler ağırlık markerları kullanılmıştır. Ortalama band sayısı 8-9, fakat jeldeki büyüklük ve yerleri değişkendir. Dendrograma gore aynı menşeye sahip P6 ve P8 aynı grupta yer alırken P1, P2, P3, P4, P5, ve P7 ikinci grupta yer almıştır. Populasyon 6 ve 8 farklı alanlardan alınmış ve aralarında benzerliğin çok fazla olduğu gözlenmiştir. Aynı şekilde populasyon P1 ve P4 fiziksel olarak birbirine yakın olmamasına rağmen çok az varyasyon göstermiştir. Belkide aynı çevreden ekotiplerin dağılımı kuşlar aracılığı ile olmuştur. SDS-PAGE analiz tekniği populasyonların tanımlanmasında oldukça faydalıdır ve populasyon ve varyete ayrımlarında kullanılabileceği gibi tohum kalite denemelerinde de kullanılabilir.

Himalayalarda bulunan çok amaçlı kullanılan Elaeagnus umbellata (Thunb.) populasyonunun SDS-PAGE ile evrimsel açıdan incelenmesi

Elaeagnus umbellata (Thunb.), commonly known as autumn olive, belongs to the family Elaeagnaceae and is native to the Himalayan regions of Pakistan, China, and India and is also found in Korea and Japan. The seeds of 8 ecotypes from Azad Jammu and Kashmir, Pakistan, were analyzed at UPM Malaysia for comparisons of their relationship and evolution based on SDS-PAGE of total seed proteins. The results indicated that each autumn olive population can be distinguished by their own specific protein bands with reference to a molecular weight marker included in the gel. The dendrogram based on computer package analysis indicated that populations having the same base of origin fall under 2 simultaneous groups, i.e. P6 and P8 in one group and P1, P2, P3, P4, P5, and P7 in the second group. The distribution of the populations was variable irrespective of their physical location as the seeds of the plant may have been dispersed by birds from distant places. SDS-PAGE thus provided valuable information for the identification of populations and could be utilized for population and varietals discrimination as well as seed quality test in true to type seed producing plants.

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