Evaluation of antiinflammatory and antinociceptive effects of some Juniperus species growing in Turkey

Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a significant antiinflammatory effect (26.9%) at a dose of 100 mg/kg. Moreover, the n-butanol subextract of Joso berry ethanol extract exhibited a promising antiinflammatory effect at 100 mg/kg. The same subextract displayed significant antinociceptive activity without inducing any gastric damage or apparent acute toxicity. Results demonstrated that the n-butanol subextract of Joso berries and the ethanol extract of J. foetidissima berries displayed remarkable antiinflammatory activity, and the n-butanol subextract of Joso also showed promising antinociceptive activity. Further studies are warranted to define and isolate the active compounds from these species to be used in the treatment of various inflammatory disorders.

Anahtar Kelimeler:

Key words: Antiinflammatory, antinociceptive, carrageenan, Juniperus excelsa, Juniperus foetidissima, Juniperus oxycedrus subsp. oxycedrus, Juniperus sabina, p-benzoquinone

Evaluation of antiinflammatory and antinociceptive effects of some Juniperus species growing in Turkey

Keywords:

Key words: Antiinflammatory, antinociceptive, carrageenan, Juniperus excelsa, Juniperus foetidissima, Juniperus oxycedrus subsp. oxycedrus, Juniperus sabina, p-benzoquinone,

PDF

___

Anilkumar M. Ethnomedicinal plants as antiinflammatory and analgesic agents. In: Chattopadhyay D. ed. Ethnomedicine: A Source of Complementary Therapeutics. Research Signpost; 2010: pp. 267–293.
Adams RP. Junipers of the World: The Genus Juniperus. Trafford Publications. Bloomington, Indiana; 2011.
Seca AML, Silva AMS. The chemical composition of the Juniperus genus (1970-2004). In: Govil JN, Singh VK, Bhardwaj R. eds. Recent Progress in Medicinal Plants, Vol. 16 - Phytomedicines. Studium Press LLC; 2006: pp. 401–522.
PDR for Herbal Medicines. Thomson Medical Economics. Montvale, New Jersey; 2000.
Barnes J, Anderson LA, Phillipson JD. Herbal Medicines. Pharmaceutical Press. London; 2007.
Karaman İ, Şahin F, Güllüce M et al. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 85: 231–235, 2003.
Fujita T, Sezik E, Tabata M et al. Traditional medicine in Turkey VII. Folk medicine in middle and west Black Sea regions. Econ Bot 49: 406–422, 1995. 8. Honda G, Yeşilada E, Tabata M et al. Traditional medicine in Turkey IV. Folk medicine in West Anatolia: Afyon, Kütahya, Denizli, Muğla, Aydın provinces. J Ethnopharmacol 53: 75–87, 1996.
Tuzlacı E, Erol MK. Turkish folk medicinal plants, part II: Eğirdir (Isparta). Fitoterapia 70: 593–610, 1993.
Yeşilada E, Honda G, Sezik E et al. Traditional medicine in Turkey V. Folk medicine in the inner Taurus Mountains. J Ethnopharmacol 46: 133–152, 1995.
Yeşilada E, Sezik E, Honda G et al. Traditional medicine in Turkey IX: Folk medicine in north-west Anatolia. J Ethnopharmacol 64: 195–210, 1999.
Okun R, Liddon SC, Lasagnal L. The effect of aggregation, electric shock and adrenergic blocking drugs on inhibition of the “writhing syndrome”. J Pharmacol Exp Ther 139: 107–109, 1963.
Yeşilada E, Küpeli E. Berberis crataegina DC. root exhibits potent anti-inflammatory, analgesic and febrifuge effects in mice and rats. J Ethnopharmacol 79: 237–248, 2002.
Akkol EK, Güvenç A, Yesilada E. A comparative study on the antinociceptive and anti-inflammatory activities of five Juniperus taxa. J Ethnopharmacol 125: 330–336, 2009.
Schepetkin IA, Faulkner CL, Nelson-Overton LK et al. Macrophage immunomodulatory activity of polysaccharides isolated from Juniperus scopolorum. Int Immunopharmacol 5: 1783–1799, 2005.
El-Sayed AM. Diterpene constituents of Juniperus polycarpos and their antimicrobial and anti-inflammatory activities. Zagazig J Pharm Sci 7: 80–86, 1998.
Orhan N, Aslan M, Pektaş M et al. Identification of hypoglycaemic compounds from berries of Juniperus oxycedrus subsp. oxycedrus through bioactivity guided isolation. J Ethnopharmacol 139: 110– 118, 2012.
Ozaki Y. Antiinflammatory effect of tetramethylpyrazine and ferulic acid. Chem Pharm Bull 40: 954–956, 1992.
Chen X, Gu L, Shi T et al. Experimental study on the anti- inflammation effect of 3,4-oxo-isopropylidene-shikimic acid. Chinese J Inflam Trad Med 3: 16, 2008.
Erazo S, Delporte C, Negrete R et al. Constituents and biological activities of Schinus polygamus. J Ethnopharmacol 107: 395–400, 2006.
Kwon JH, Kim JH, Choi SE et al. Inhibitory effects of phenolic compounds from needles of Pinus densiflora on nitric oxide and PGE2 production. Arch Pharm Res 33: 2011–2016, 2010.
El-Seedi HR, Ringbom T, Torssell K et al. Constituents of Hypericum laricifolium and their cyclooxygenase (COX) enzyme activities. Chem Pharm Bull 51: 1439–1440, 2003.
Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenan edema in rats. J Pharmacol Exp Ther 166: 96–103, 1969.
Olajide OA, Makindo JM, Awe SO. Effects of the aqueous extract of Bridelia ferruginea stem bark on carrageenan-induced edema and granuloma tissue formation in rats and mice. J Ethnopharmacol 66: 113–117, 1999.
Baltacı S, Kolatan HE, Yılmaz O et al. Anti-inflammatory activity of Anthemis aciphylla var. aciphylla Boiss. Turk J Biol 35: 757-762, 2011.
Lekshmi UMD, Reddy PN. Preliminary studies on antiinflammatory, antipyretic, and antidiarrhoeal properties of Evolvulus alsinoides. Turk J Biol 35: 611–618, 2011.