Effects of a-lipoic acid on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in sub-chronic thinner-addicted rats

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance, the relation between toxicity and oxidative stress, and a possible protective effect of a-lipoic acid against thinner toxication in rats. Sprague-Dawley rats were divided into 5 groups as follows: control (K), olive oil (Z), a-lipoic acid (L), thinner (T), and a-lipoic acid + thinner (LAT). Each group was composed of 15 rats, and the study lasted 8 weeks. After completing the animal studies malondialdehyde (MDA), reduced glutathione (GSH), methemoglobin (MetHb), toluol, and mononuclear leucocyte damage levels; protein oxidation, nitric oxide (NOx) metabolites, total antioxidant capacity (TAC), glucose, total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined from blood specimens of the rats. The data obtained from the study were statistically analyzed using SPSS, and both ANOVA and Duncan tests were performed. P < 0.05 was accepted as statistically significant. The results indicated that a-lipoic acid has a protective and balancing effect against complications derived from thinner inhalation in rats.

Effects of α-lipoic acid on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in sub-chronic thinner-addicted rats

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance, the relation between toxicity and oxidative stress, and a possible protective effect of a-lipoic acid against thinner toxication in rats. Sprague-Dawley rats were divided into 5 groups as follows: control (K), olive oil (Z), a-lipoic acid (L), thinner (T), and a-lipoic acid + thinner (LAT). Each group was composed of 15 rats, and the study lasted 8 weeks. After completing the animal studies malondialdehyde (MDA), reduced glutathione (GSH), methemoglobin (MetHb), toluol, and mononuclear leucocyte damage levels; protein oxidation, nitric oxide (NOx) metabolites, total antioxidant capacity (TAC), glucose, total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined from blood specimens of the rats. The data obtained from the study were statistically analyzed using SPSS, and both ANOVA and Duncan tests were performed. P < 0.05 was accepted as statistically significant. The results indicated that a-lipoic acid has a protective and balancing effect against complications derived from thinner inhalation in rats.

___

  • Cobanoglu F, Ozercan IH, Ozercan MR et al. The effect of inhaling thinner and/or cigarette smoke on rat kidneys. Inhal Toxicol 19: 303−8, 2007. 2. Ulakoğlu EZ, Saygı A, Gümüstas MK et al. The effect of chronic thinner inhalation on lipid peroxidation in rat lung and liver. Cerrahpasa J Med 29: 75−8, 1998.
  • Ulakoğlu EZ, Saygı A, Gümüştaş MK et al. Alterations in superoxide dismutase activities, lipid peroxidation and glutathione levels in thinner inhaled rat lung: relationship between histopathological properties. Pharmacol Res 38(3): 209−214, 1998.
  • Yılmaz B, Kutlu S, Canpolat S et al. Effects of paint thinner exposure on serum LH, FSH and testosterone levels and hypothalamic catecholamine contents in the male rat. Biol Pharm Bull 24(2): 163−166, 2001. 5 Mattia CJ, LeBel CP, Bondy SC. Effect of toluene and its metabolites on cerebral reactive oxygen species generation. Biochem Pharmacol 42: 879-82, 1991.
  • Al-Alousi LM. Pathology of volatile substance abuse: a case report and a literature review. Med Sci Law 29: 189-20, 1989.
  • Marjot R, McLeod AA. Chronic non-neurological toxicity from volatile substance abuse. Hum Toxicol 8: 301−306, 1989.
  • Lebel CP, Schatz RA. Altered synaptosomal phospholipid metabolism after toluene. Possible relationship with membrane fluidity, Na+, K+ - adenosine triphosphate and phospholipid methylation. J Pharmacol Exp Ther 253: 1189−97, 1990. 9. Mattia CJ, Adams JD, Bondy SC. Free radical induction in the brain and liver by products of toluene catabolism. Biochem Pharmacol 46: 103−10, 1993.
  • Mattia CJ, Ali SF, Bondy SC. Toluene-induced oxidative stress in several brain regions and other organs. Mol Chem Neuropathol 3: 313−28, 1993.
  • Dizdaroglu M. Chemical determination of free radical-induced damage to DNA. Free Radical Biol Med 10: 225−242, 1991.
  • Shigenaga MK, Ames BN. Assays for 8-hydroxy-2’- deoxyguanosine: a biomarker of in vivo oxidative DNA damage. Free Radical Biol Med 10: 211−216, 1991.
  • Kuchino Y, Mori F, Kasai H et al. Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues. Nature 327: 77−79, 1987.
  • Cheng KC, Cahil DS, Nishimura S et al. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G to T and A to C substitutions. J Biol Chem 267: 166−172, 1992.
  • Perham N. Domains, motifs and linkers in 2-oxo-acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein. Biochemistry 30: 8501−8512, 1991.
  • Biewenga G, Haenen GR, Bast A. The pharmacology of the antioxidant lipoic acid. Gen Pharmacol 29: 315−331, 1997.
  • Kocyigit A, Keles H, Selek S et al. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Res 585 (1−2): 71−78, 2005.
  • Ostling O, Johanson KJ. Microelectrophoretic study of radiation induced DNA damage in individual mammalian cells. Biochem Bioph Res Co 123: 291–98, 1984.
  • Singh NP, McCoy MT, Tice RR et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184−191, 1988.
  • Collins AR. The comet assay for DNA damage and repair principles, applications, and limitations. Mol Biotechnol 26: 249−261, 2004.
  • Kobayashi H, Sugiyama C, Morikava Y et al. A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis. MMS Commun 3(2): 103−115, 1995.
  • Horoz M, Bolukbas C, Bolukbas F et al. Assessment of peripheral DNA damage by alkaline comet assay in maintenance hemodialysis subjects with hepatitis C infection. Mutat Res 596(1−2): 137−142, 2006.
  • Yurtcu E, Kasapoğlu E, Şahin Fİ. Protective effects of β-carotene and silymarin on human lymphocytes. Turk J Biol 36: 47−52, 2012.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Method Enzymol 186: 421−30, 1990.
  • Harrison JH, Jollow DJ. Role of aniline metabolites in aniline induced hemolytic anemia. J Pharmacol Exp Ther 238: 1045−1054, 1986.
  • Levin RL, Garland D, Oliver CN et al. Determination of carbonyl content in oxidatively modified proteins. Method Enzymol 186: 464−478, 1990.
  • Ceylan E, Kocyigit A, Gencer M et al. Increased DNA damage in patients with chronic obstructive pulmonary disease who had once smoked or been exposed to biomass. Resp Med 100: 1270−1276, 2006.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882−888, 1963.
  • Miranda KM, Espey G, Wink AD. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. NATO Sci S A Lif Sci 5(1): 62−71, 2001.
  • Bülbül A. Mastitisli İnek Sütlerinde Nitrik Oksit Düzeyi İle Somatik Hücre Sayısı Arasındaki İlişki. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara, 2003.
  • Özdamar K. SPSS ile Biyoistatistik. 5. Baskı. Kaan Kitap Evi. Eskişehir; 2003.
  • Yamada K. Influence of lacquer thinner and some organic solvent on reproductive and accessory reproductive organs in male rat. Biol Pharm Bull 16(4): 425−7, 1993.
  • Rudich A, Tirosh A, Potashnik R et al. Lipoic acid protects against oxidative stress induced impairment in insulin stimulation of protein kinase B and glucose transport in 3T3- L1 adipocytes. Diabetologia 42: 949–957, 1999.
  • Thirunavukkarasuru V, Anitha NAT, Anuradha CV. Lipoic acid improves glucose utilization and prevents protein glycation and AGE formation. Pharmazie 60(10): 772−5, 2005.
  • Guzelian P, Mills S, Fallon HJ. Liver structure and function in print workers exposed to toluene. J Occup Med 30(10): 791−796, 1988.
  • Bozic T, Stevanovic J, Kovacevic J et al. Toluene mediated oxidative stress and granulo - monocytopoiesis. Acta Vet- Beograd 534: 201−10, 2003.
  • Dündaroz MR, Türkbay T, Akay C et al. Antioxidant enzymes and lipid peroxidation in adolescents with inhalant abuse. Turk J Pediatr 45: 43−5, 2003.
  • Çelik S, Yılmaz Ö. The effects of dietary vitamin E on sera lipids and lipid peroxida peroxidation in diabetic rats. Turk J Biol 23(1): 39−46, 1999.
  • Halifeoğlu I, Karatas F, Üstündag B et al. Tiner ile çalısan kişilerde tiner solumanın antioksidan vitaminler üzerine etkisi. Turk J Biochem 24: 29−33, 1999.
  • Çelik H. Malarya (Sıtma) Hastalarında Oksidatif stres ve Mononüklear Lenfosit DNA Hasarının Araştırılması. Yüksek Lisans Tezi, Harran Üniversitesi Sağlık Bilimleri Enstitüsü, Şanlıurfa, 2005.
  • Alfaro MM, Palma TL, Sandoval ZF et al. Correlation between formamidopyrimidine DNA glycosylase (Fpg)-sensitive sites determined by a comet assay, increased MDA, and decreased glutathione during long exposure to thinner inhalation. Toxicol Lett 163(3): 198−205, 2006.
  • Ripine JE, Bast A, Lankhorst I et al. Oxidative stress in chronic obstructive pulmonary disease. J Respir Crit Care Med 156(26): 341–347, 1997.
  • Aksoy Y. Antioksidan mekanizmada glutatyonun rolü (The role of glutathione in antioxidant mechanism). Turk Klin Tıp Bilim 22: 442−448, 2002.
  • Amudha G, Josephine A, Varalakshmi P. Role of lipoic acid in reducing the oxidative stress induced by cyclosporine A. Clin Chim Acta 372: 1−2, 134−139, 2006.
  • Elangovan S, Chidambaram P, Periyasamy TS et al. Chemoprotective effect of lipoic acid against cyclophosphamide- induced changes in the rat sperm. Toxicology 217: 1, 71−78, 2006.
  • Maniscalco M, Grieco L, Galdi A et al. Increase in exhaled nitric oxide in shoe and leather workers at the end of the work- shift. Occup Med (London) 54(6): 404−407, 2004.
  • Matthew BG, Jourd’Heuil D, Wink DA. Nitric oxide I. Physiological chemistry of nitric oxide and its metabolites: implications in inflammation. Am J Physiol 276: 315−321, 1999.
  • Alfaro MM, Contreras YA, Trejo AC et al. Oxidative stress effects of thinner inhalation. Indian J Occup Environ Med 15(3): 87−92, 2011.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Biological screening of various medicinal plant extracts for antibacterial and antitumor activities

Fatma PEHLİVAN KARAKAŞ, Arzu YILDIRIM, Arzu TÜRKER

Utilization of gold nanostructures in biomedical applications

Gamze TAN, Mehmet Ali ONUR, Necdet SAĞLAM

Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique

Mehmet NİYAZ, Özer Aylin GÜRPINAR, Serdar GÜNAYDIN, Mehmet Ali ONUR

Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp. hybrid cv. HSF-240 under salt stress

Batool EJAZ, Zahoor Ahmad SAJID, Faheem AFTAB

Purification and properties of an endoglucanase from Aspergillus niger VTCC-F021

Thi Hoa PHAM, Dinh Thi QUYEN, Ngoc Minh NGHIEM

Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Allium cepa L.

Sonia SHARMA, Avinash NAGPAL, Adarsh Pal VIG

A comparison of major taste- and health-related compounds of Vaccinium berries

Jasminka MILIVOJEVIC, Vuk MAKSIMOVIC, Jelena DRAGISIC MAKSIMOVIC

Dipeptidyl peptidase IV production by solid state fermentation using alternative fungal sources

Gaye ÖNGEN, Sayıt SARGIN, Özlem ÜSTÜN, Ceren KUTLU, Mesut YÜCEL

Evaluation of nested PCR method for diagnosis of meningitis due to Neisseria meningitidis and Haemophilus influenzae

Zahra HOJJATI BONAB, Safar FARAJNIA, Reza GHOTASLOU, Elhameh NIKKHAH

Evaluation of antiinflammatory and antinociceptive effects of some Juniperus species growing in Turkey

Nilüfer ORHAN, Esra AKKOL, Fatma ERGUN