Azizuddin SHAIKH, Muhammad Iqbal CHOUDHARY

Bioassay studies of 2ʹ-O-ethylmurrangatin isolated from a medicinal plant, Murraya paniculata

A secondary metabolite, 2ʹ-O-ethylmurrangatin (1), was isolated from the leaves of a medicinal plant, Murraya paniculata. The structure of compound 1 was identified with the help of spectroscopic techniques. Compound 1 was screened for its antioxidant, lipoxygenase, prolyl endopeptidase, and respiratory burst inhibitory activities. 2ʹ-O-Ethylmurrangatin (1) was found to have significant activity against lipoxygenase enzyme and moderate respiratory burst inhibitory activity, but no activity against either DPPH radical scavenging assay or prolyl endopeptidase enzyme.

PDF

___

1. Sastri BN. The Wealth of India, Council of Scientific and Industrial Research, New Delhi, Vol. 6: 446-448, 1962.
2. Perry LM, Metzger J. Medicinal Plants of East and Southeast Asia. The Massachusetts Institute of Technology Press. Cambridge; 1980. p. 367,
3. Choudhary MI, Azizuddin, Khalid A et al. A new coumarin from Murraya paniculata. Planta Med 68: 81-83, 2002.
4. Richard AL. The antioxidants of higher plants. Phytochemistry 27: 969-979, 1988.
5. Floyd RA. DNA-Ferrous iron catalyzed hydroxyl free radical formation from hydrogen peroxide. Biochem Biophys Res Commun 99: 1209-1215, 1981.
6. Shi H, Hiramatsu M, Kamatsu M et al. Antioxidant property of Fructus momordicae extract. Biochem Mol Biol Intl 40: 1111- 1121, 1996.
7. Gross MD, Snowdon D, Snowdon A. Plasma antioxidant concentrations in a population of elderly women: findings from the nun study. Nutrition Research 16: 1881-1890, 1996.
8. Kim YD, Chen B, Beauregard J et al. 17 beta-Estradiol prevents dysfunction of canine coronary endothelium and myocardium and reperfusion arrhythmias after brief ischemia/reperfusion. Circulation 94: 2901-2908, 1996.
9. Land WEM. Mechanisms of action of anti-inflammatory drugs. Advance Drug Research 14: 147-167, 1985.
10. Steinhilber D. 5-Lipoxygenase: A target for anti-inflammatory drugs revisited. Curr Med Chem 6: 71-85, 1999.
11. Nie D, Honn KV. Cyclooxygenase, lipoxygenase and tumor angiogenesis. Cellular and Molecular Life Sciences 59: 799- 807, 2002.
12. Fan W, Komatsu K, Tezuka Y et al. Prolyl endopeptidase inhibitors from the underground part of Rhodiola sacra S. H. Fu. Biol Pharm Bull 22: 157-161, 1999.
13. Szeltner Z, Renner V, Polgar L. Substrate- and pH-dependent contribution of oxyanion binding site to the catalysis of prolyl oligopeptidase, a paradigm of the serine oligopeptidase family. Protein Science 9: 353-360, 2000.
14. Fujita Y, Uehara F, Morimoto Y et al. Studies on inhibition mechanism of autoxidation by tannins and flavonoids. Yakugaku Zasshi 108: 129-135, 1998.
15. Smith RC, Reeves JC, Dage RC et al. Antioxidant properties of 2-imidazolones and 2-imidazolthiones. Biochem Pharmacol 36: 1457-1460, 1987.
16. Tappel AL. Methods in Enzymology, Academic Press, New York, Vol. 05: pp. 539, 1962.
17. Yoshimoto T, Walter R, Suru T. Proline-specific endopeptidase from Flavobacterium. Purification and properties. J Biol Chem 255: 4786-4792, 1980.
18. Tan AS, Berridge VM. Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: a simple colorimetric assay for measuring respiratory burst activation and for screening antiinfl ammatory agents. J Immunol Methods 238, 50-68, 2000.
19. Siddiqui RA, English D, Cui Y et al. Phorbal ester-induced priming of superoxide generation by phosphotidic acidstimulated neutrophils and granule-free neutrophil cytoplasts. J Leuko Biol 58: 189-195, 1995.
20. Choudhary MI, Azizuddin, Jalil S et al. Inhibition of respiratory burst in human neutrophils and lipoxygenase enzyme by compounds from Haloxylon griffithii. Phytotherapy Research 20: 840-843, 2006.