A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential oil of O. onites decreased cancer cell viability at higher concentrations. The essential oil was found to be less cytototoxic (IC50: 149.12 µg/mL) than carvacrol and thymol (IC50: 53.09 and 60.01 µg/mL, respectively) for Hep G2 cells. On the other hand, with the O. onites essential oil, carvacrol and thymol protected the cells against H2O2-induced cytotoxicity when the cells were preincubated with the oil and its components at a lower concentration (

Anahtar Kelimeler:

Key words: O. onites, essential oil, antioxidant, anticancer

A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

Keywords:

Key words: O. onites, essential oil, antioxidant, anticancer,

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Duthie GG, Brown KM. Reducing the risk of cardiovascular disease. In: Goldberg I. ed. Functional Foods: Designer Foods, Pharmafoods, Nutraceuticals. Chapman & Hall; 1998: pp. 19- 38.
Milner JA. Reducing the risk of cancer. In: In: Goldberg I. ed. Functional Fo ods: Designer Foods, Pharmafoods, Nutraceuticals. Chapman & Hall; 1998: pp. 39-70. 3. Gülçin İ, Kireçci E, Akkemik E et al. Antioxidant, antibacterial, and anticandidal activities of an aquatic plant: duckweed (Lemna minor L. Lemnaceae). Turk J Biol 34: 175-188, 2010.
Kutlu T, Durmaz G, Ateş B et al. Antioxidant properties of diff erent extracts of black mulberry (Morus nigra L.). Turk J Biol 35: 103-110, 2011.
Seyoum A, Asres K, El-Fiky FK. Structure-radical scavenging activity relationship of fl avonoids. Phytochem 67: 2058-2070, 2006.
Ozkan A, Erdogan A, Sokmen M et al. Antitumoral and antioxidant eff ect of essential oils and in vitro antioxidant properties of essential oils and aqueous extracts from Salvia pisidica. Biologia 65: 990-996, 2010.
Lagouri V, Blekas G, Tsimidou M et al. Composition and antioxidant activity of essential oils from Oregano plants grown wild in Greece. Z Lebensm Unter Forsch 197: 20-23, 1993.
Toncer O, Karaman S, Kizil S et al. Changes in essential oil composition of Oregano (Origanum onites L.) due to diurnal variations at diff erent development stages. Not Bot Hort Agrobot Cluj 37: 177-181, 2009.
Özkum D, Kürkçüoğlu M, Başer KHC et al. Essential oils from wild and micropropagated plants of Origanum minutifl orum O. Schwarz et Davis. J Essent Oil Res22: 135-137, 2010.
Baser KHC. Th e Turkish Origanum species. In: Kintzios SE. ed. Oregano, the Genera Origanum and Lippia. Taylor & Francis; 2002: pp. 109-126.
Ka H, Park HJ, Jung HJ et al. Cinnamaldehyde induces apoptosis by ROS-mediated mitochondrial permeability transition in human promeolcytic leukaemia HL-60 cell. Cancer Lett 196: 143-152, 2003.
Griffi ths DE. Psi-screen, an in vitro toxicity test system: applications in the bioassay of perfumes and fragrance chemicals. Altern Lab Anim 33: 471-486, 2005.
Duchen MR. Mitochondria in health and disease: perspectives on a new mitochondrial biology. Mol Aspects Med 25: 365- 451, 2004.
Özbek T, Güllüce M, Şahin F et al. Investigation of the antimutagenic potentials of the methanol extract of Origanum vulgare L. subsp. vulgare in the Eastern Anatolia Region of Turkey. Turk J Biol 32: 271-276, 2008.
Azcan N, Kara M, Asilbekova DT et al. Lipids and essential oil of Origanum onites. Chem Natural Comp 36: 132-136, 2000.
Ultee A, Kets E, Smid EJ. Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 65: 4606-4610, 1997.
Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 94: 223-253, 2004.
Lambert RJV, Skandamis PN, Coote PJ et al. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J App Microbiol 91: 453-462, 2001.
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 14: 323-328, 2000.
Cuendet M, Hostettmann K, Potterat O et al. Iridoid glycosides with free radical scavenging properties from Fagraea blumei. HCA 80: 1144-1152, 1997.
Dapkevicius A, Venskutonis R, van Beek TA et al. Antioxidant activity of extracts obtained by diff erent isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 77: 140-146, 1998.
Gloeckner H, Jonuleit T, Lemke HD. Monitoring of cell viability and cell growth in a hollow-fi ber bioreactor by use of the dye alamarBlue. J Immunol Methods 252: 131-138, 2001.
Wasowicz W, Neve J, Peretz A. Optimized steps in fl uorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and infl uence of sample preservation and storage. Clin Chem 39: 2522-2526, 1993.
Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248-254, 1976.
Kirkman TW 1996. Statistics to Use (online). Available at http://www.physics.csbsju.edu/stats/1996 (accessed 17 August 2008).
Dorman HJD, Surai P, Deans SG. In vitro antioxidant activity of a number of plant essential oils and phytoconstituents. J Essent Oil Res 12: 241-248, 2000.
Ruberto G, Barata MT. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem 69: 167-174, 2000.
Kaya GI, Ünver Somer N, Konyalıoğlu S et al. Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turk J Biol 34: 139-146, 2010.
Isfahlan AJ, Mahmoodzadeh A, Hassanzadeh A et al. Antioxidant and antiradical activities of phenolic extracts from Iranian almond (Prunus amygdalus L.) hulls and shells. Turk J Biol 34: 165-173, 2010.
Demirci F, Demirci B, Gürbüz İ et al. Characterization and biological activity of Achillea teretifolia Willd. and A. nobilis L. subsp neilreichii (Kerner) Formanek essential oils. Turk J Biol 33: 129-136, 2009.
Öğütçü H, Sökmen A, Sökmen M et al. Bioactivities of the various extracts and essential oils of Salvia limbata C.A.Mey. and Salvia sclarea L. Turk J Biol 32: 181-192, 2008.
Aydin S, Basaran AA, Basaran N. Modulating eff ects of thyme and its major ingredients on oxidative DNA damage in human lymphocytes. J Agric Food Chem 53: 1299-1305, 2005.
Slamenova D, Horvathova E, Sramkova M et al. DNA- protective eff ects of two components of essential plant oils carvacrol and thymol on mammalian cells cultured in vitro. Neoplasma 54: 108-112, 2007.
Reddy BS, Wang CX, Samaha H et al. Chemoprevention of colon carcinogenesis by dietary perillyl alcohol. Cancer Res 57: 420-425, 1997.
Koparal AT, Zeytinoglu M. Eff ects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549. Animal Cell Technology: Basic & Applied Aspects 13: 207-211, 2003.
Mastelic J, Jerkovic I, Blazevic I et al. Comparative study on the zantioxidant and biological activities of carvacrol, thymol, and eugenol derivatives. J Agric Food Chem 56: 3989-3996, 2008.