Alam KHAN, Moizur RAHMAN, Shariful ISLAM

Antibacterial, Antifungal and Cytotoxic Activities of Tuberous Roots of Amorphophallus campanulatus

Antibacterial, antifungal and cytotoxic activities of ethanol extract of tuberous roots of Amorphophallus campanulatus were studied. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The extract showed significant antibacterial activities against four gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Streptococcus b-haemolyticus) and six gram-negative bacteria (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhi). The MIC values against these bacteria ranged from 16 to 128 µg/ml. The antifungal activity was found weak against the tested fungi. In cytotoxicity determination, LC50 of the extract against brine shrimp nauplii was 7.66 µg/ml.

Anahtar Kelimeler:

Amorphophallus campanulatus, ethanol extract, gram-positive, gram-negative, MIC, cytotoxicity, antifungal activity

Antibacterial, Antifungal and Cytotoxic Activities of Tuberous Roots of Amorphophallus campanulatus

Keywords:

Amorphophallus campanulatus, ethanol extract, gram-positive, gram-negative, MIC, cytotoxicity, antifungal activity,

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1. Al-Bari MAA, Sayeed MA, Rahman MS et al. Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis, a novel species collected in Bangladesh. Res J Medicine & Med Sci 1: 77-81, 2006.
2. Rahman MM, Wahed MII, Biswas MHU et al. In vitro antibacterial activity of the compounds of Trapa bispinosa Roxb. The Science 1: 214-216, 2001.
3. Bhattacharya S. Chrinjib banoushadi. Vol. 2, 1st ed. Anand Publishing Ltd. Calcutta, India; 1990: pp. 63-69.
4. Ghani A. Medicinal Plants of Bangladesh. Asiatic Society of Bangladesh. Dhaka, Bangladesh; 1998: pp. 77-78.
5. Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. 4, 2nd ed. Dehra Dun Publisher Ltd. India; 1994: pp. 2609-2610.
6. Trease EG, Evans WC. Textbook of Pharmacognosy. 14th ed. W.B. Saunders Company. UK; 1997: p. 119.
7. Jeffery GH, Bassett J, Mendham J et al. Vogel’s Textbook of Quantitative Chemical Analysis. 5th ed. Longman Group UK Ltd. England; 2000: p. 161.
8. Dongmo AB, Ndom JC, Massoma LD et al. Vasodilating effect of the root of bark extract of Ficus saussureana on guinea pig aorta. Pharm Biol 41: 371-374, 2003.
9. Kivack B, Mert T, Tansel H. Antimicrobial and cytotoxic activities of Ceratonia siliqua L. extracts. Turk J Biol 26: 197-200, 2001.
10. Carson CF, Hammer KA, Riley TV. Broth microdilution method for determination of susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca altenifolia (tea tree oil). Microbios 82: 181-185, 1995.
11. Dash S, Nath LK, Bhise S et al. Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. Trop J Pharm Res 4: 341-347, 2005.
12. Rahman MM, Mosaddik MA, Wahed MII et al. Antimicrobial activity and cytotoxicity of Trapa bispinosa. Fitoterapia 71: 704- 706, 2000.
13. Mosaddik MA, Haque ME. Cytotoxicity and antimicrobial activity of goniothalamin isolated from Bryonopsis laciniosa. Phytother Res 17: 1155-1157, 2003.
14. Hossain MS, Hossain MA, Islam R et al. Antimicrobial and cytotoxic activities of 2-aminobenzoic acid and 2-aminophenol and their coordination complexes with magnesium (Mg-II). Pak J Biol Sci 71: 25-27, 2004.
15. Islam MA, Sayeed MA, Islam MA et al. Terpenes from bark of Zanthoxylum budrunga and their cytotoxic activities. Rev Latinoamer Quím 30: 24-28, 2002.
16. Finney DJ. Probit Analysis. 3rd ed. University Press. Cambridge, UK; 1971: pp. 18, 37-77.
17. Al-Bari MAA, Sayeed MA, Khan A et al. In vitro antimicrobial activities and cytotoxicity of ethyl acetate extract from Streptomyces maritimus. Biotechnology 6: 81-85, 2007.
18. Gurkan E, Tuzun OT, Hirlak F. Cytotoxicity assay of some papaver alkaloids using Artemia salina (Brine shrimp). Fitoterapia LXVI: 544-545, 1995.
19. Martin-Cordero G, Saenz MT, Ayuso MJ. Cytotoxic activity of Retama spaerocarpa. Fitoterapia XVI: 495-498, 1995. 20. Mongelli E, Desmarchelier C, Giulietti A. Bioactivity of certain medicinal latexes used by the Eséejas. J Ethnopharmacol 47: 159- 163, 1995.
21. Desmarchelier C, Mongelli E, Coussio J et al. Studies on the cytotoxicity, antimicrobials and NA-binding activities of plants used by the Eséejas. J Ethnopharmacol 50: 91-96, 1996.
22. Mutalik S, Paridhavi K, Rao CM et al. Antipyretic and analgesic effect of leaves of Solanum Melongena Linn. in rodents. Indian J Pharmacol 35: 312-315, 2003.